一所高等院校污水系统中 SARS-CoV-2 的流行病学评估。

IF 2.6 4区 医学 Q2 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Annals of Global Health Pub Date : 2024-08-07 eCollection Date: 2024-01-01 DOI:10.5334/aogh.4413
Carmem Cícera Maria da Silva, Carolina Rangel de Lima Santos, Eliomar Pivante Céleri, David Salles, Julia Miranda Fardin, Kamily Fagundes Pussi, Daniel Claudio de Oliveira Gomes, Vinicius de Oliveira Ribeiro, Leila Cristina Konrad-Moraes, Herintha Coeto Neitzke-Abreu, Valdemar Lacerda Júnior
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引用次数: 0

摘要

背景:世界卫生组织在采取全球紧急措施三年后,于 2023 年 5 月宣布 COVID-19 大流行结束。监测污水中的 SARS-CoV-2 因其有效性和成本效益而显得尤为重要,这也凸显了优先开展水资源和卫生研究的必要性。目标:本研究旨在对位于圣埃斯皮里图州维托里亚市 Maruípe 校区的一所高等教育机构的污水系统中的 SARS-CoV-2 进行流行病学评估。评估方法在 2023 年 2 月 6 日至 4 月 12 日的 66 天内,采集了 15 个样本。每个样本 1 升,1 小时采集一次,每 15 分钟采集 250 毫升。通过评估样本的外观来确定样本的特征,并使用 Horiba U-50 多参数探头测量 pH 值。使用 Allplex™ 2019-nCovAssay Seegene 试剂盒对提取的 RNA 进行 RT-qPCR 分析。结果:样品外观浑浊,含有杂质,pH 值在 6.35 至 8.17 之间。在评估的样本中,有两份样本检测到了 SARS-CoV-2 RNA,通过与州卫生局发布的流行病学公告进行比较,发现在这些样本采集期间,该州的病例有所增加。结论事实证明,污水监测是疫情后时期的一项重要工具,可作为新疫情爆发时的预警和预防机制。此外,它还代表了一种低成本的绘图策略和对人群的广泛测试,与大流行初期的研究结果一致。考虑到不同的人群,我们建议做出具体调整。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An Epidemiological Assessment of SARS-CoV-2 in the Sewage System of a Higher Education Institution.

Background: The World Health Organization declared the end of the COVID-19 pandemic in May 2023, three years after the adoption of global emergency measures. Monitoring of SARS-CoV-2 in sewage underscores its importance due to its effectiveness and cost-effectiveness, highlighting the need to prioritize research on water resources and sanitation. Objectives: The aim of this study was to conduct an epidemiological assessment of SARS-CoV-2 in the sewage system of a higher education institution located in Vitória Espírito Santo State, Maruípe campus. Methods: Over a period of 66 days, from February 6 to April 12, 2023, 15 samples were collected. Each sample consisted of 1 L, collected in 1 hour, with 250 mL collected every 15 minutes. The samples were characterized by assessing their appearance, and pH was measured using a Horiba U-50 multiparameter probe. The extracted RNA was subjected to RT-qPCR using the Allplex™ 2019-nCovAssay Seegene kit. Results: The samples exhibited a cloudy appearance with impurities, and the pH ranged from 6.35 to 8.17. Among the evaluated samples, SARS-CoV-2 RNA was detected in two, and, by comparing this with the epidemiological bulletin issued by the State Health Department, an increase in cases in the state was observed during the collection period of these samples. Conclusions: Sewage monitoring proved to be an important tool in this post-pandemic period, serving as an alert and prevention mechanism for the population in relation to new outbreaks. Furthermore, it represents a low-cost mapping strategy and extensive testing of a population, aligning with the studies presented at the beginning of the pandemic. We recommend specific adjustments considering distinct populations.

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来源期刊
Annals of Global Health
Annals of Global Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-
CiteScore
5.30
自引率
3.40%
发文量
95
审稿时长
11 weeks
期刊介绍: ANNALS OF GLOBAL HEALTH is a peer-reviewed, open access journal focused on global health. The journal’s mission is to advance and disseminate knowledge of global health. Its goals are improve the health and well-being of all people, advance health equity and promote wise stewardship of the earth’s environment. The journal is published by the Boston College Global Public Health Program. It was founded in 1934 by the Icahn School of Medicine at Mount Sinai as the Mount Sinai Journal of Medicine. It is a partner journal of the Consortium of Universities for Global Health.
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