梓醇对血管生成的促进作用及其潜在机制:基于网络药理学的研究

IF 3.2 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jin-rong Ni, Qun-hu Zhang, Jie-lin Deng, Hai-hu Wang, Yong-chi Duan, Cheng-ji Zhang, Lue-tao Jiang
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引用次数: 0

摘要

梓醇是一种天然鸢尾甙,具有潜在的治疗功效,包括抗炎和神经保护作用。调查梓醇在血管生成中的作用对于了解其潜在的治疗应用至关重要,尤其是在调节血管生成有益的疾病中。本研究结合网络药理学和体外实验,对 catalpol 对血管生成的影响及其机制进行了研究。通过 SwissTargetPrediction 对 catalpol 对应的靶基因进行了分析。然后从 GeneCards 等数据库中获取与血管生成相关的靶基因。随后,利用注释、可视化和综合发现数据库(Database for Annotation, Visualization and Integrated Discovery)进行基因本体论和通路分析,并利用Cytoscape将蛋白质相互作用可视化。使用细胞计数试剂盒-8 和血管生成试验进一步检测了催化酚对 HUVECs 存活率和血管生成的影响。应用 RT-qPCR 和 Western 印迹检测血管生成相关蛋白的表达。共获得了 312 个 catalpol 靶基因和 823 个血管生成相关靶基因,其中 56 个共同靶基因导致了 PPI 网络分析,突出了枢纽基因(AKT1、表皮生长因子受体、STAT3、MAPK3 和 CASP3)。这些中心基因主要富集在脂质和动脉粥样硬化通路以及表皮生长因子受体相关通路中。体外实验结果表明,梓醇可实现 HUVECs 活力的浓度依赖性增加。梓醇还能促进 HUVECs 的迁移和血管生成,并上调表皮生长因子受体的表达。表皮生长因子受体敲除抑制了梓醇对 HUVECs 的作用。梓醇通过上调表皮生长因子受体和血管生成相关蛋白促进 HUVECs 的血管生成,这表明它在血管相关疾病中具有潜在的治疗应用价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Promotion Effect of Catalpol on Angiogenesis and Potential Mechanisms: A Research Based on Network Pharmacology

Promotion Effect of Catalpol on Angiogenesis and Potential Mechanisms: A Research Based on Network Pharmacology

Catalpol, a natural iridoid glycoside, has potential therapeutic benefits, including anti-inflammatory and neuroprotective effects. Investigating catalpol's role in angiogenesis is critical for understanding its potential therapeutic applications, particularly in diseases where modulating angiogenesis is beneficial. This study investigates catalpol's influence on angiogenesis and its mechanisms, combining network pharmacology and in vitro experiments. The target genes corresponding to the catalpol were analyzed by SwissTargetPrediction. Then angiogenesis-related targets were acquired from databases like GeneCards. Subsequently, the Database for Annotation, Visualization and Integrated Discovery was employed for Gene Ontology and pathway analysis, while Cytoscape visualized protein interactions. The effect of catalpol on viability and angiogenesis of HUVECs was further examined using Cell Counting Kit-8 and angiogenesis assays. RT-qPCR and western blot were applied to check the expression of angiogenesis-related proteins. Totally, 312 target genes of catalpol and 823 angiogenesis-related targets were obtained with 56 common targets leading to PPI network analysis, highlighting hub genes (AKT1, EGFR, STAT3, MAPK3, and CASP3). These hub genes were mainly enriched in lipid and atherosclerosis pathway and EGFR-related pathway. The in vitro experimental results showed that catalpol achieved a concentration-dependent increase in HUVECs viability. Catalpol also promoted the migration and angiogenesis of HUVECs and up-regulated the expression of EGFR. EGFR knockdown inhibited the effect of catalpol on HUVECs. Catalpol promotes angiogenesis in HUVECs by upregulating EGFR and angiogenesis-related proteins, indicating its potential therapeutic application in vascular-related diseases.

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来源期刊
Chemical Biology & Drug Design
Chemical Biology & Drug Design 医学-生化与分子生物学
CiteScore
5.10
自引率
3.30%
发文量
164
审稿时长
4.4 months
期刊介绍: Chemical Biology & Drug Design is a peer-reviewed scientific journal that is dedicated to the advancement of innovative science, technology and medicine with a focus on the multidisciplinary fields of chemical biology and drug design. It is the aim of Chemical Biology & Drug Design to capture significant research and drug discovery that highlights new concepts, insight and new findings within the scope of chemical biology and drug design.
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