心脏组织的转录组比较分析揭示了绵羊对海拔适应性的不同基因表达谱

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Ritika Gera , Reena Jagati Arora , Pooja Chhabra , Upasna Sharma , Ram Parsad , Sonika Ahlawat , Mohsin Ayoub Mir , Manoj Kumar Singh , Rekha Sharma , Rajesh Kumar
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引用次数: 0

摘要

本研究旨在阐明适应印度高原地区和低洼地区的绵羊心脏组织的转录组变化。Changthangi绵羊生长在拉达克寒冷干旱的高原地区,而Muzzafarnagri绵羊原产于亚热带平原,其特点是酷热和高湿度。每个品种的绵羊心脏组织都有四个生物重复,用于生成 RNA 测序数据。这两个品种的绵羊都表现出与关键心脏功能(如肌肉收缩、肥大和钙离子平衡)相关基因的高表达。差异基因表达分析确定了与海拔适应相关的不同表达谱,Changthangi羊的凋亡信号和Muzzafarnagri羊的糖胺聚糖代谢都很丰富。基因共表达网络突显了凋亡和表皮生长因子受体通路的重要性,它们可能协调了高海拔地区昌唐吉羊对低氧水平的适应性反应。在 Muzzafarnagri 羊中发现的节点基因在应激反应和 MAPK 信号通路中发挥着重要作用,是细胞在不利条件下适应和生存的关键。我们研究中发现的关键基因为今后研究绵羊的海拔适应性提供了令人信服的候选基因。这项综合分析为了解绵羊心脏对高海拔的适应性的遗传基础提供了宝贵的见解,揭示了参与高海拔环境生理调整的分子途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative transcriptome analyses of cardiac tissue reveals differential gene expression profiles in sheep in response to altitudinal adaptation

The present study aims to elucidate the transcriptomic changes occurring in the cardiac tissue of sheep breeds acclimated to highland versus lowland regions in India. The Changthangi sheep thrives in cold arid highlands of Ladakh in contrast to the Muzzafarnagri sheep which is native to the sub-tropical plains characterized by sweltering heat and high humidity. Four biological replicates of heart tissue from each breed were used to generate the RNA sequencing data. Both breeds exhibited high expression of genes related to crucial cardiac functions such as muscle contraction, hypertrophy and calcium ion homeostasis. Differential gene expression analysis identified distinct expression profiles associated with altitudinal adaptation, with enrichment of apoptotic signals in Changthangi and glycosaminoglycan metabolism in Muzzafarnagri sheep. Gene co-expression network highlighted the significance of apoptosis and EGFR pathway that may orchestrate adaptive responses to low oxygen levels in Changthangi sheep found in high altitude. The nodal genes identified in Muzzafarnagri sheep play essential roles in stress responses and MAPK signaling pathways, pivotal for cellular adaptation and survival under adverse conditions. The key genes identified within our study present compelling candidates for future studies in altitudinal adaptation in ovines. This comprehensive analysis provides valuable insights into the genetic basis of cardiac adaptation to altitude in sheep, shedding light on the molecular pathways involved in physiological adjustments to high-altitude environments.

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来源期刊
Small Ruminant Research
Small Ruminant Research 农林科学-奶制品与动物科学
CiteScore
3.10
自引率
11.10%
发文量
210
审稿时长
12.5 weeks
期刊介绍: Small Ruminant Research publishes original, basic and applied research articles, technical notes, and review articles on research relating to goats, sheep, deer, the New World camelids llama, alpaca, vicuna and guanaco, and the Old World camels. Topics covered include nutrition, physiology, anatomy, genetics, microbiology, ethology, product technology, socio-economics, management, sustainability and environment, veterinary medicine and husbandry engineering.
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