开发重氮烷基标签,利用光亲和标记分析蛋白质与配体的相互作用

IF 1.4 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Masatoshi Nakatani, Shohei Ebihara, Masaki Kita
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引用次数: 0

摘要

我们开发了一种重氮烷基标签,用于分析目标蛋白质与配体之间的相互作用。我们利用铜催化叠氮-炔环加成(CuAAC)技术轻松制备了磺酰胺光亲和探针,该探针能与人碳酸酐酶 1 特异性地形成共价键(标记率∼30%)。光标记位置(E58-H64 区域)是根据胰蛋白酶肽串联质谱分析结果确定的,并得到了分子模型研究的支持。我们的方法可用于确定各种目标生物大分子的未知配体结合位点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of diazirine–alkyne tag for protein–ligand interaction analysis with photoaffinity labeling
We have developed a diazirine–alkyne tag for target protein–ligand interaction analysis. A sulfonamide photoaffinity probe was readily prepared using copper-catalyzed azide–alkyne cycloaddition (CuAAC), which site-specifically formed a covalent bond with human carbonic anhydrase 1 (∼30% labeling yield). The photolabeled position (E58–H64 region) was determined based on the results of a tandem mass spectrometry (MS/MS) analysis of tryptic peptides and was supported by molecular modeling studies. Our method may be useful for determining unknown ligand-binding sites of various target biomolecules.
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来源期刊
Chemistry Letters
Chemistry Letters 化学-化学综合
CiteScore
3.00
自引率
6.20%
发文量
260
审稿时长
1.2 months
期刊介绍: Chemistry Letters covers the following topics: -Organic Chemistry- Physical Chemistry- Inorganic Chemistry- Analytical Chemistry- Materials Chemistry- Polymer Chemistry- Supramolecular Chemistry- Organometallic Chemistry- Coordination Chemistry- Biomolecular Chemistry- Natural Products and Medicinal Chemistry- Electrochemistry
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