育雏期种鸡粪便中大肠杆菌耐药水平、多重耐药模式和耐药基因的演变

Alejandro Fenollar-Penadés, Pablo Catalá-Gregori, Vicente Tallá-Ferrer, María Ángeles Castillo, Miguel García-Ferrús, Ana Jiménez-Belenguer
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引用次数: 0

摘要

食物链是抗生素耐药性进入人类和环境的入口。鉴于家禽业的重要性,我们对饲养期间从 14500 只种鸡及其饲养环境中分离出的大肠埃希菌进行了调查,以了解抗生素耐药性的流行和演变情况。样本包括来自一天龄种鸡的胎粪,以及来自小母鸡和成年母鸡育种舍的粪便样本和靴子拭子。一天龄雏鸡的所有大肠杆菌分离物(77% 来自粪便,61% 来自靴拭子)都对至少一种抗生素具有耐药性。在育雏期间,粪便分离物对头孢他啶和多种药物的耐药性分别从一天龄雏鸡的 41.2% 和 80.8% 降至成年鸡的 3.8% 和 33.8%。从粪便和靴拭子中的大肠杆菌中检测到了所有研究基因,最常见的是 blaTEM(75%)、blaSHV(72%)和 qnrB(67%)。68.7%的粪便和靴拭子分离物中检测到至少一种头孢菌素和一种喹诺酮类药物的耐药基因组合。我们的研究结果突出表明,有必要对养殖场的抗生素耐药性流行情况进行监测,并采取适当措施降低对公众和环境健康的风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evolution of the Antibiotic Resistance Levels, Multi-Resistance Patterns, and Presence of Antibiotic Resistance Genes in E. coli Isolates from the Feces of Breeding Hens during the Rearing Period
The food chain acts as an entry point for antibiotic resistance to reach humans and environment. Because of the importance of the poultry sector, we investigated the prevalence and evolution of antibiotic resistance in Escherichia coli isolates from a series of 14,500 breeding hens and their farm environment during the rearing period. Samples included meconium from one-day-old breeders and fecal samples and boot swabs from the breeding sheds of pullets and adult hens. All E. coli isolates from one-day-old chicks, 77% from feces and 61% from boot swabs, were resistant to at least one antibiotic. Cefotaxime and multi-drug resistance in fecal isolates decreased during the rearing period from 41.2% and 80.8% in one-day-old chicks to 3.8% and 33.8% in adults. All genes studied were detected in E. coli from feces and boot swabs, the most common being blaTEM (75%), blaSHV (72%), and qnrB (67%). blaCMY-2 was detected in 100% of one-day-old breeders. The combination of at least one cephalosporin and one quinolone resistance gene was detected in 68.7% of fecal and boot swab isolates. Our results highlight the need to monitor the prevalence of antibiotic resistance on farms and to take appropriate measures to reduce the risk to public and environmental health.
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