农田中抗生素抗性基因的调控取决于有机添加剂的选择和掠夺性细菌的盛行程度

Anna Karin Rosberg, Maria João Silva, Cecilie Skøtt Feidenhans’l, Eddie Cytryn, Edouard Jurkevitch, Rolf Lood
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摘要

抗生素耐药性基因(ARGs)在环境中广泛存在,特别是土壤,是具有固有抗生素耐药性微生物的热点。农业生产中用作肥料的粪便和污泥已被证明含有大量 ARGs,由于持续施用,ARGs 在农业土壤中不断积累。不过,有些土壤具有复原能力,这可能取决于特定的土壤特性,以及是否存在能够水解活细菌(包括对临床有重要意义的细菌)的捕食性细菌。本研究的目的是:(i) 探讨添加牛粪、鸡粪和污泥是否会对土壤微生物群的抗生素耐药性产生不同影响;(ii) 探讨添加物是否会对捕食性细菌的存在产生影响。将三种有机添加剂分别与田间土壤混合,装入盆中,在温室中培养 28 天。使用液滴数字 PCR(ddPCR)对三种 ARGs、两种捕食细菌和细菌总数进行量化。这项研究表明,有机改良剂的选择会显著影响土壤的抗生素耐药性状况,并促进捕食性细菌的生长,而细菌总数则不受影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Regulation of Antibiotic Resistance Genes on Agricultural Land Is Dependent on Both Choice of Organic Amendment and Prevalence of Predatory Bacteria
Antibiotic resistance genes (ARGs) are widespread in the environment, and soils, specifically, are hotspots for microorganisms with inherent antibiotic resistance. Manure and sludge used as fertilizers in agricultural production have been shown to contain vast amounts of ARGs, and due to continued applications, ARGs accumulate in agricultural soils. Some soils, however, harbor a resilience capacity that could depend on specific soil properties, as well as the presence of predatory bacteria that are able to hydrolyse living bacteria, including bacteria of clinical importance. The objectives of this study were to (i) investigate if the antibiotic resistance profile of the soil microbiota could be differently affected by the addition of cow manure, chicken manure, and sludge, and (ii) investigate if the amendments had an effect on the presence of predatory bacteria. The three organic amendments were mixed separately with a field soil, divided into pots, and incubated in a greenhouse for 28 days. Droplet digital PCR (ddPCR) was used to quantify three ARGs, two predatory bacteria, and total number of bacteria. In this study, we demonstrated that the choice of organic amendment significantly affected the antibiotic resistance profile of soil, and promoted the growth of predatory bacteria, while the total number of bacteria was unaffected.
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