利用相位敏感光学相干断层扫描技术进行体内视网膜测温

Yueming Zhuo, Mohajeet Balveer Bhuckory, Huakun Li, Hattori Junya, Davis Pham-Howard, David Veysset, Tong Ling, Daniel Palanker
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引用次数: 0

摘要

控制视网膜激光治疗过程中的组织温升对于获得可预测的治疗效果至关重要,尤其是在无损伤的情况下。我们展示了一种利用相位分辨光学相干断层扫描(pOCT)在体内确定视网膜温升的方法。根据 10 毫秒激光脉冲期间视网膜中的热诱导光路长度变化(ΔOPL)进行测量,可以检测到精度小于 1 °C的温升,这足以校准针对特定患者的非损伤性治疗的激光功率。我们观察到正常视网膜和变性视网膜在视网膜变形的局限性方面存在显著差异:在野生型大鼠中,热变形位于视网膜色素上皮(RPE)和感光器内节(IS)之间,而在变性视网膜中,变形则深入视网膜内层。这意味着在健康的感光体中存在一种结构成分,可以抑制激光加热 RPE 和色素脉络膜所引起的组织膨胀。我们假设,连接感光器内外节段(IS、OS)的薄而软的纤毛可吸收 OS 的变形,从而阻止组织进一步向内扩张。健康视网膜和变性视网膜在激光脉冲诱导的视网膜变形限制方面的惊人差异,可作为一种生物力学诊断工具,用于描述感光器变性的特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Retinal thermometry in-vivo using phase-sensitive optical coherence tomography
Controlling the tissue temperature rise during retinal laser therapy is essential for predictable outcomes, especially at non-damaging settings. We demonstrate a method for determining the temperature rise in the retina using phase-resolved optical coherence tomography (pOCT) in vivo. Measurements based on the thermally induced optical path length changes (ΔOPL) in the retina during a 10-ms laser pulse allow detection of the temperature rise with a precision less than 1 °C, which is sufficient for calibration of the laser power for patient-specific non-damaging therapy. We observed a significant difference in confinement of the retinal deformations between the normal and the degenerate retina: in wild-type rats, thermal deformations are localized between the retinal pigment epithelium (RPE) and the photoreceptors' inner segments (IS), as opposed to a deep penetration of the deformations into the inner retinal layers in the degenerate retina. This implies the presence of a structural component within healthy photoreceptors that dampens the tissue expansion induced by the laser heating of the RPE and pigmented choroid. We hypothesize that the thin and soft cilium connecting the inner and outer segments (IS, OS) of photoreceptors may absorb the deformations of the OS and thereby preclude the tissue expansion further inward. Striking difference in the confinement of the retinal deformations induced by a laser pulse in healthy and degenerate retina may be used as a biomechanical diagnostic tool for the characterization of photoreceptor degeneration.
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