{"title":"利用干热麦拉德反应方法改变经右旋糖酐超声预处理的向日葵蛋白质异构体的功能和结构特性","authors":"Rashmi Rawat, Charanjiv Singh Saini","doi":"10.1007/s11947-024-03547-1","DOIUrl":null,"url":null,"abstract":"<p>Sunnhemp protein isolate (SHPI) was prepared by utilizing alkaline extraction and acid precipitation method, followed by ultrasound treatment at 50% amplitude for 20 min having fixed power, frequency, and pulse ratio of 500 W, 20 kHz, 15 s, respectively. The ultrasound pretreated SHPI was conjugated with dextran (1:1 w/w ratio) by dry-heating method of Maillard reaction at 60 °C for 0, 1, 3, 5, 7, and 9 days of incubation at 79% relative humidity. The functional properties of SHPI-dextran conjugates like solubility, emulsifying, foaming, water- and oil-binding capacities, dispersibility, and gelation were improved as compared to pure SHPI. Increments in browning index values of SHPI-dextran conjugates were observed with an increase in Maillard reaction time. The α-helix content of conjugated SHPI reduced, while the β-sheet, β-turn, and random coils content increased. FTIR spectroscopy confirmed the formation of covalent bonds between SHPI and dextran via Maillard reaction. XRD analysis indicated both the semicrystalline and amorphous structure of SHPI-dextran conjugates as the incubation time was increased from 0 to 9 days. The decreasing trend in the values of <i>H</i><sub>0</sub> values was found with an increase in incubation time. Free and total sulfhydryl content of SHPI was increased after conjugation with dextran for up to 5 days and thereafter decreased. The incubation time of 5 days at 60 °C and 79% RH was optimized on the basis of improvement in functional characteristics and extent of Maillard reaction time. Overall, the present study showed that conjugation with dextran successfully improved the functional characteristics of SHPI.</p>","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":"30 1","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Utilizing Dry-Heating Maillard Reaction Approach to Modify the Functional and Structural Properties of Ultrasound Pretreated Sunnhemp Protein Isolate with Dextran\",\"authors\":\"Rashmi Rawat, Charanjiv Singh Saini\",\"doi\":\"10.1007/s11947-024-03547-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Sunnhemp protein isolate (SHPI) was prepared by utilizing alkaline extraction and acid precipitation method, followed by ultrasound treatment at 50% amplitude for 20 min having fixed power, frequency, and pulse ratio of 500 W, 20 kHz, 15 s, respectively. The ultrasound pretreated SHPI was conjugated with dextran (1:1 w/w ratio) by dry-heating method of Maillard reaction at 60 °C for 0, 1, 3, 5, 7, and 9 days of incubation at 79% relative humidity. The functional properties of SHPI-dextran conjugates like solubility, emulsifying, foaming, water- and oil-binding capacities, dispersibility, and gelation were improved as compared to pure SHPI. Increments in browning index values of SHPI-dextran conjugates were observed with an increase in Maillard reaction time. The α-helix content of conjugated SHPI reduced, while the β-sheet, β-turn, and random coils content increased. FTIR spectroscopy confirmed the formation of covalent bonds between SHPI and dextran via Maillard reaction. XRD analysis indicated both the semicrystalline and amorphous structure of SHPI-dextran conjugates as the incubation time was increased from 0 to 9 days. The decreasing trend in the values of <i>H</i><sub>0</sub> values was found with an increase in incubation time. Free and total sulfhydryl content of SHPI was increased after conjugation with dextran for up to 5 days and thereafter decreased. The incubation time of 5 days at 60 °C and 79% RH was optimized on the basis of improvement in functional characteristics and extent of Maillard reaction time. Overall, the present study showed that conjugation with dextran successfully improved the functional characteristics of SHPI.</p>\",\"PeriodicalId\":562,\"journal\":{\"name\":\"Food and Bioprocess Technology\",\"volume\":\"30 1\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2024-08-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food and Bioprocess Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11947-024-03547-1\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Bioprocess Technology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11947-024-03547-1","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Utilizing Dry-Heating Maillard Reaction Approach to Modify the Functional and Structural Properties of Ultrasound Pretreated Sunnhemp Protein Isolate with Dextran
Sunnhemp protein isolate (SHPI) was prepared by utilizing alkaline extraction and acid precipitation method, followed by ultrasound treatment at 50% amplitude for 20 min having fixed power, frequency, and pulse ratio of 500 W, 20 kHz, 15 s, respectively. The ultrasound pretreated SHPI was conjugated with dextran (1:1 w/w ratio) by dry-heating method of Maillard reaction at 60 °C for 0, 1, 3, 5, 7, and 9 days of incubation at 79% relative humidity. The functional properties of SHPI-dextran conjugates like solubility, emulsifying, foaming, water- and oil-binding capacities, dispersibility, and gelation were improved as compared to pure SHPI. Increments in browning index values of SHPI-dextran conjugates were observed with an increase in Maillard reaction time. The α-helix content of conjugated SHPI reduced, while the β-sheet, β-turn, and random coils content increased. FTIR spectroscopy confirmed the formation of covalent bonds between SHPI and dextran via Maillard reaction. XRD analysis indicated both the semicrystalline and amorphous structure of SHPI-dextran conjugates as the incubation time was increased from 0 to 9 days. The decreasing trend in the values of H0 values was found with an increase in incubation time. Free and total sulfhydryl content of SHPI was increased after conjugation with dextran for up to 5 days and thereafter decreased. The incubation time of 5 days at 60 °C and 79% RH was optimized on the basis of improvement in functional characteristics and extent of Maillard reaction time. Overall, the present study showed that conjugation with dextran successfully improved the functional characteristics of SHPI.
期刊介绍:
Food and Bioprocess Technology provides an effective and timely platform for cutting-edge high quality original papers in the engineering and science of all types of food processing technologies, from the original food supply source to the consumer’s dinner table. It aims to be a leading international journal for the multidisciplinary agri-food research community.
The journal focuses especially on experimental or theoretical research findings that have the potential for helping the agri-food industry to improve process efficiency, enhance product quality and, extend shelf-life of fresh and processed agri-food products. The editors present critical reviews on new perspectives to established processes, innovative and emerging technologies, and trends and future research in food and bioproducts processing. The journal also publishes short communications for rapidly disseminating preliminary results, letters to the Editor on recent developments and controversy, and book reviews.