{"title":"从大肠杆菌培养物中生产和提取高良酸的优化方案","authors":"S. A. Tsvetikova, E. I. Koshel","doi":"10.1134/s1068162024040344","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p><b>Objective:</b> Colanic acid (CA) is a promising and actively studied polysaccharide, which geroprotective and stimulating properties have already been described in animal models. Different protocols are used to isolate and purify CA in research, some of which may result in contamination with proteins, DNA, and other polysaccharides. <b>Methods:</b> In order to effectively further investigate the biological properties of CA, we developed 5 protocols for CA isolation, differed in cultivation condition, protein precipitation, and polysaccharide precipitants. <b>Results and Discussion:</b> We found that the most suitable protocol for CA isolation includes protein removal with trichloroacetic acid and polysaccharide precipitation with ethanol (95%, 3 volumes). <b>Conclusions:</b> Such a simple protocol will serve to standardize CA studies and can be applied to obtain large volumes of polysaccharide.</p>","PeriodicalId":758,"journal":{"name":"Russian Journal of Bioorganic Chemistry","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimized Protocol for Production and Extraction of Colanic Acid from E. coli Culture\",\"authors\":\"S. A. Tsvetikova, E. I. Koshel\",\"doi\":\"10.1134/s1068162024040344\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Abstract</h3><p><b>Objective:</b> Colanic acid (CA) is a promising and actively studied polysaccharide, which geroprotective and stimulating properties have already been described in animal models. Different protocols are used to isolate and purify CA in research, some of which may result in contamination with proteins, DNA, and other polysaccharides. <b>Methods:</b> In order to effectively further investigate the biological properties of CA, we developed 5 protocols for CA isolation, differed in cultivation condition, protein precipitation, and polysaccharide precipitants. <b>Results and Discussion:</b> We found that the most suitable protocol for CA isolation includes protein removal with trichloroacetic acid and polysaccharide precipitation with ethanol (95%, 3 volumes). <b>Conclusions:</b> Such a simple protocol will serve to standardize CA studies and can be applied to obtain large volumes of polysaccharide.</p>\",\"PeriodicalId\":758,\"journal\":{\"name\":\"Russian Journal of Bioorganic Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2024-08-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Russian Journal of Bioorganic Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1134/s1068162024040344\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Bioorganic Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1134/s1068162024040344","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
摘要目的:可乐定酸(CA)是一种前景广阔、研究活跃的多糖,已在动物模型中描述了其老年保护和刺激特性。研究中使用了不同的方案来分离和纯化 CA,其中一些方案可能会导致蛋白质、DNA 和其他多糖的污染。方法:为了有效地进一步研究CA的生物学特性,我们开发了5种分离CA的方案,它们在培养条件、蛋白质沉淀和多糖沉淀剂方面各不相同。结果与讨论我们发现最适合的 CA 分离方案包括用三氯乙酸去除蛋白质和用乙醇(95%,3 体积)沉淀多糖。得出结论:这种简单的方案将有助于实现 CA 研究的标准化,并可用于获取大量多糖。
Optimized Protocol for Production and Extraction of Colanic Acid from E. coli Culture
Abstract
Objective: Colanic acid (CA) is a promising and actively studied polysaccharide, which geroprotective and stimulating properties have already been described in animal models. Different protocols are used to isolate and purify CA in research, some of which may result in contamination with proteins, DNA, and other polysaccharides. Methods: In order to effectively further investigate the biological properties of CA, we developed 5 protocols for CA isolation, differed in cultivation condition, protein precipitation, and polysaccharide precipitants. Results and Discussion: We found that the most suitable protocol for CA isolation includes protein removal with trichloroacetic acid and polysaccharide precipitation with ethanol (95%, 3 volumes). Conclusions: Such a simple protocol will serve to standardize CA studies and can be applied to obtain large volumes of polysaccharide.
期刊介绍:
Russian Journal of Bioorganic Chemistry publishes reviews and original experimental and theoretical studies on the structure, function, structure–activity relationships, and synthesis of biopolymers, such as proteins, nucleic acids, polysaccharides, mixed biopolymers, and their complexes, and low-molecular-weight biologically active compounds (peptides, sugars, lipids, antibiotics, etc.). The journal also covers selected aspects of neuro- and immunochemistry, biotechnology, and ecology.