膳食补充剂中虾青素的 TLC 色谱定量及其抗氧化活性研究

IF 2.8 4区 工程技术 Q2 ENGINEERING, CHEMICAL
Processes Pub Date : 2024-08-11 DOI:10.3390/pr12081680
Iwona Dymek, Joanna Żandarek, Małgorzata Starek, Monika Dąbrowska
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引用次数: 0

摘要

虾青素是一种红色类胡萝卜素色素,具有很强的抗氧化和免疫调节功能,其含量高于其他类胡萝卜素。本研究旨在对含有虾青素的膳食补充剂进行定性和定量评估。首先,开发了使用 TLC 技术和密度检测进行分析的最佳条件。流动相由甲醇:乙酸乙酯:流动相为甲醇:乙酸乙酯:1,4-二氧六环(1:3:6 v/v/v),固定相为硅胶 60 F254。在 460 纳米波长下进行密度检测。接下来,根据国际协调会议(ICH)的指导方针对所开发的方法进行了验证。线性范围为 0.0026-0.0100 µg/spot, LOD 和 LOQ 分别为 0.85 和 2.57 ng/μL。变异系数为 4.75%,证明该方法具有良好的精密度。回收率为 95.25%-104.94%。所得结果证实了该方法的良好准确性。随后,对制剂进行了定量分析。对膳食补充剂的分析表明,其虾青素含量与申报含量有明显偏差。虾青素溶液在碱性环境中以及暴露于光和氧化性物质时是稳定的,但在酸性环境中会降解。已进行的抗氧化能力测试证实虾青素具有很高的抗氧化活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studies of TLC-Chromatographic Quantification of Astaxanthin in Dietary Supplements and Its Antioxidant Activity
Astaxanthin is a red carotenoid pigment known for its strong antioxidant and immune-supporting properties, which are higher than other carotenoids. The aim of this study was the qualitative and quantitative evaluation of dietary supplements containing astaxanthin. First, optimal conditions for conducting analyses using the TLC technique with densitometric detection were developed. The mobile phase consisting of methanol: ethyl acetate: 1,4-dioxane (1:3:6 v/v/v) was selected, while the stationary phase consisted of Silica gel 60 F254. Densitometric detection was performed at 460 nm. Next, the validation process of the developed method was carried out according to the guidelines of the International Conference on Harmonization (ICH). The range of linearity tested was 0.0026–0.0100 µg/spot, and the determined LOD and LOQ values were 0.85 and 2.57 ng/μL, respectively. The variation coefficient at the level of 4.75% proves good precision. The percentage of recovery was in the range of 95.25–104.94%. The obtained results confirmed the good accuracy of the method. Subsequently, quantitative analyses of the preparations were carried out. Analysis of dietary supplements showed significant deviations from the declared astaxanthin content. Astaxanthin solutions were stable in alkaline environments and when exposed to light and oxidizing substances; however, the substance degraded in acidic environments. The performed antioxidant capacity tests confirmed the high antioxidant activity of astaxanthin.
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来源期刊
Processes
Processes Chemical Engineering-Bioengineering
CiteScore
5.10
自引率
11.40%
发文量
2239
审稿时长
14.11 days
期刊介绍: Processes (ISSN 2227-9717) provides an advanced forum for process related research in chemistry, biology and allied engineering fields. The journal publishes regular research papers, communications, letters, short notes and reviews. Our aim is to encourage researchers to publish their experimental, theoretical and computational results in as much detail as necessary. There is no restriction on paper length or number of figures and tables.
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