富含叶绿素的 Ascophyllum nodosum 海藻提取物可抑制流感感染

bioRxiv Pub Date : 2024-08-08 DOI:10.1101/2024.08.08.606782
D. F. Mega, Parul Sharma, Anja Kipar, U. Hetzel, Chloe Bramwell, Alan Merritt, Samuel Wright, Chris Plummer, R. Urbanowicz, James P. Stewart
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引用次数: 0

摘要

海藻提取物是一种可再生资源,可用于制造业和食品工业。本研究的重点是从 Ascophyllum nodosum 中分离出的一种富集海藻提取物(ESE)。ESE 通过斑块还原试验对甲型流感病毒(IAV)H1N1 和 H3N2 亚型进行了抗病毒活性筛选。添加时间测定和 FACS 分析用于帮助确定作用模式。然后,利用处于气液间期的分化人支气管上皮细胞和受到 IAV 病毒挑战的小鼠模型,探讨了 ESE 的治疗潜力。数据显示,ESE 主要与病毒直接相互作用,阻止病毒与细胞结合。有趣的是,在细胞结合后进行处理时,ESE 还能抑制甲型流感生命周期的早期和晚期阶段。这种抑制作用似乎是通过靶向神经氨酸酶的活性来阻止病毒内化和后代病毒的释放。对感染 IAV 的小鼠进行 ESE 鼻内给药可减少肺组织中的病毒载量。ESE 可能是治疗流感感染的一种前景广阔的广谱抗病毒药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phlorotannin rich Ascophyllum nodosum seaweed extract inhibits influenza infection
Seaweed derived compounds are a renewable resource utilised in the manufacturing and food industry. This study focuses on an Enriched seaweed extract (ESE) isolated from Ascophyllum nodosum. ESE was screened for antiviral activity by plaque reduction assays against influenza A viruses (IAV) H1N1 and H3N2 subtypes. Time of addition assays and FACS analysis were used to help determine the modes of action. The therapeutic potential of ESE was then explored using differentiated human bronchiole epithelial cells at the air liquid interphase and a murine model challenged with IAV. The data indicates ESE primarily interacts directly with virions, preventing virus cell binding. Interestingly, ESE also inhibits early and late stage of the influenza A lifecycle when treatment occurs after cell binding. This inhibitory effect appears to prevent internalisation of virus and release of progeny virus by targeting neuraminidase activity. Intranasal administration of ESE in mice infected with IAV reduced viral load in lung tissue. ESE may be a promising broad acting antiviral agent in the treatment of influenza infections.
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