Nelson N. Obaretin, C. Daokoru-Olukole, J. Pondei, E. Fenibo, Philip O. Okerentugba, Herbert O. Stanley, C. D. Onwukwe
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引用次数: 0
摘要
由于重金属对细菌有抑制作用,重金属污染会扭曲和削弱微生物的多样性,导致易感细菌被淘汰,抗性菌株大量繁殖,最终成为优势菌株。因此,本研究试图描述从受重金属污染的垃圾场土壤中分离出的优势细菌(RCBBR_B37 和 RCBBR_B38)的特征。使用 Zymo Quick DNA 真菌/细菌试剂盒从分离物中提取 DNA,并使用 NanoDrop 2000c 分光光度计评估提取 DNA 的纯度。凝胶电泳证实了 DNA 条带的存在,其中包括一条对照条带。随后,使用 27F 和 1492R 互补引物对提取的 DNA 进行了 PCR 扩增。PCR 产物经过序列分析后,在美国国家生物技术信息中心(NCBI)数据库中进行了 BLAST 搜索。根据 BLAST 结果构建了系统发生树。RCBBR_B37 与真菌芽孢杆菌的相似指数为 98%,而 RCBBR_B38 与副黏液芽孢杆菌的相似指数为 97%。重金属污染垃圾场中普遍存在芽孢杆菌是一个特征,代表了微生物在改善重金属方面的不同相互作用机制,包括但不限于生物累积、固存、氧化还原转化、中和、代谢和解毒。
Molecular Assessment of Dominant Genus in Heavy Metal Contaminated Soil from Selected Dumpsites in Port Harcourt, Nigeria
Heavy metal contamination distorts and diminishes microbial diversity due to their inhibitory effects on bacteria, resulting in the elimination of susceptible bacteria and the proliferation of resistant strains, which eventually become dominant. Consequently, this study seeks to characterize the dominant bacteria (RCBBR_B37 and RCBBR_B38) isolated from soil at a dump site contaminated with heavy metals. DNA extraction from the isolates was performed using the Zymo Quick DNA Fungal/Bacterial Kit, and the purity of the extracted DNA was assessed using a NanoDrop 2000c spectrophotometer. Gel electrophoresis confirmed the presence of DNA bands, including a control band. Subsequently, PCR amplification using 27F and 1492R complementary primers was carried out on the extracted DNA. The PCR products underwent sequence analysis followed by BLAST search in the National Center for Biotechnology Information (NCBI) database. A phylogenetic tree was constructed based on the BLAST results. Isolate RCBBR_B37 exhibited a 98% similarity index to Bacillus fungorum, while RCBBR_B38 showed a 97% similarity index to Bacillus paramycoides. The prevailing presence of Bacillus in heavy metal contaminated dumpsite is a signature, representing the difference mechanisms of microbial interaction for heavy metals amelioration, including but not limited to bioaccumulation, sequestration, redox transformation, neutralization, metabolization, and detoxification.