青蒿提取物是逆转大鼠模型中糖尿病心肌病的新型治疗方法

Pharmaceuticals Pub Date : 2024-08-08 DOI:10.3390/ph17081046
Liza, G. Hussain, Abdul Malik, Suhail Akhtar, Haseeb Anwar
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引用次数: 0

摘要

糖尿病心肌病是一种严重的糖尿病并发症,会损害心脏功能,导致心力衰竭。目前亟需一种既能有效治疗这种病症,又不会产生副作用的治疗方法。目前的抗高血糖疗法价格昂贵,有副作用,而且不能有效防止心脏重塑。因此,探索有可能逆转心脏重塑的天然产品非常重要。因此,本研究的目的是确定青蒿甲醇提取物在糖尿病心肌病大鼠模型中的左心室重塑潜力。在对植物酚类和类黄酮含量进行了初步的综合植物化学评估,结果显示其具有很强的抗高血糖和抗氧化活性之后,我们在体内实验中给大鼠服用了青蒿提取物。按照之前文献中描述的方法诱导 Wistar 白化大鼠患糖尿病心肌病,45 天后通过血清和组织病理学分析检测治疗效果。青蒿治疗可明显降低空腹血糖(108.5 ± 1.75 mg/dL)、糖化血红蛋白(4.03 ± 0.12 %)、血清葡萄糖(116.66 ± 3.28 mg/dL)、胰岛素(15.66 ± 0.66 ng/mL)、总氧化状态(54.66 ± 3.22 µmol H2O2Equiv.L-1)、丙二醛(0.20 ± 0.01 mmol/L)、总胆固醇(91.16 ± 3.35 mg/dL)、甘油三酯(130.66 ± 3.15 mg/dL)、低密度脂蛋白(36.57 ± 1.02 mg/dL)、钠(140 ± 3.21 mmol/L)、钙(10.44 ± 0.24 mmol/L)、肌酸激酶 MB(1227.5 ± 17.89 IU/L)、乳酸脱氢酶(1300 ± 34.64 IU/L)、C 反应蛋白(30 ± 0.57 pg/mL)、肿瘤坏死因子-α(58.66 ± 1.76 pg/mL)、心房钠尿肽(2.53 ± 0.04 pg/mL)、B 型钠尿肽(10.66 ± 0.44 pg/mL)、天门冬氨酸氨基转移酶(86.5 ± 4.99 U/L)、丙氨酸转氨酶(55.33 ± 2.90 U/L)、尿素(25.33 ± 1.15 mg/dL)和肌酐(0.64 ± 0.02 mg/dL),但总抗氧化能力(1.73 ± 0.07 mmol Trolox Equil./L)、高密度脂质(40 ± 1.59 mg/dL)和钾(3.82 ± 0.04 mmol/L)水平显著升高(p≤0.05)。心电图和组织病理学证实,心脏和胰腺的重塑和结构变化得到了显著改善和逆转。总之,青蒿在糖尿病诱发的心肌病过程中具有显著的左心室重塑潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Artemisia vulgaris Extract as A Novel Therapeutic Approach for Reversing Diabetic Cardiomyopathy in a Rat Model
Diabetic cardiomyopathy, a severe diabetic complication, impairs heart function, leading to heart failure. Treatment that effectively addresses this condition without causing side effects is urgently needed. Current anti-hyperglycemic therapies are expensive, has side effects and do not effectively prevent cardiac remodeling. Therefore, it is important to explore natural products that may have the potential to reverse cardiac remodeling. That is why the aim of the current study was to determine the left ventricular remodeling potential of the methanolic extract of Artemisia vulgaris in a diabetic cardiomyopathy rat model. Following the initial comprehensive phytochemical evaluation of plant phenolic and flavonoid content, which showed strong anti-hyperglycemic and antioxidant activities, an extract of Artemisia vulgaris was administered in an in vivo experiment. Diabetic cardiomyopathy was induced in Wistar albino rats according to previously described protocols in the literature, and the effect of treatment was checked by serum and histopathological analysis after 45 days. Artemisia vulgaris treatment significantly (p ≤ 0.05) reduced fasting blood glucose (108.5 ± 1.75 mg/dL), glycated hemoglobin (4.03 ± 0.12 %), serum glucose (116.66 ± 3.28 mg/dL), insulin (15.66 ± 0.66 ng/mL), total oxidant status (54.66 ± 3.22 µmol H2O2Equiv.L−1), Malondialdehyde (0.20 ± 0.01 mmol/L), total cholesterol (91.16 ± 3.35 mg/dL), triglycerides (130.66 ± 3.15 mg/dL), low-density lipids (36.57 ± 1.02 mg/dL), sodium (140 ± 3.21 mmol/L), calcium (10.44 ± 0.24 mmol/L), creatine kinase MB (1227.5 ± 17.89 IU/L), lactate dehydrogenase (1300 ± 34.64 IU/L), C-reactive protein (30 ± 0.57 pg/mL), tumor necrosis factor-α (58.66 ± 1.76 pg/mL), atrial natriuretic peptide (2.53 ± 0.04 pg/mL), B-type natriuretic peptide (10.66 ± 0.44 pg/mL), aspartate aminotransferase (86.5 ± 4.99 U/L), Alanine Transaminase (55.33 ± 2.90 U/L), urea (25.33 ± 1.15 mg/dL) and creatinine (0.64 ± 0.02 mg/dL) but significantly increased (p ≤ 0.05) total antioxidant capacity (1.73 ± 0.07 mmol Trolox Equil./L), high-density lipids (40 ± 1.59 mg/dL) and potassium (3.82 ± 0.04 mmol/L) levels. ECG and histopathology confirmed the significant improvement in remodeling and the reversal of structural changes in the heart and pancreas. In conclusion, Artemisia vulgaris possesses significant left ventricular remodeling potential in course of diabetes-induced cardiomyopathy.
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