盐水提取物和富含蛋白质的 Moringa oleifera Lam.叶

Pharmaceuticals Pub Date : 2024-08-08 DOI:10.3390/ph17081045
Robson Raion de Vasconcelos Alves, Alisson Macário de Oliveira, Gabryella Borges dos Prazeres, Abdênego Rodrigues da Silva, Franciele Florencio Costa, B. R. S. Barros, T. G. Souza, L. Coelho, C. D. de Melo, Magda RA Ferreira, L. Soares, C. Chagas, Maria Lígia Rodrigues Macedo, T. Napoleão, Mariana Pinheiro Fernandes, P. Paiva
{"title":"盐水提取物和富含蛋白质的 Moringa oleifera Lam.叶","authors":"Robson Raion de Vasconcelos Alves, Alisson Macário de Oliveira, Gabryella Borges dos Prazeres, Abdênego Rodrigues da Silva, Franciele Florencio Costa, B. R. S. Barros, T. G. Souza, L. Coelho, C. D. de Melo, Magda RA Ferreira, L. Soares, C. Chagas, Maria Lígia Rodrigues Macedo, T. Napoleão, Mariana Pinheiro Fernandes, P. Paiva","doi":"10.3390/ph17081045","DOIUrl":null,"url":null,"abstract":"Moringa oleifera Lam. (horseradish tree) leaves demonstrate high nutritional value, are rich in proteins, and are widely used in folk medicine and food. This study investigated the presence of secondary metabolites and antinutritional proteins in leaf extract (LE) and the protein-rich fraction (PRF) derived from M. oleifera leaves, as well as the cytotoxicity to human cells, hemolytic activity, and in vivo acute toxicity and genotoxicity in mice. The flavonoids rutin and vitexin as well as trypsin inhibitors and lectins were detected in LE and PRF. Neither sample demonstrated toxicity against human peripheral blood mononuclear cells and both showed low hemolytic action. In vivo, LE and PRF did not show antinutritional effects and caused no death. The hematological parameters of the animals in the treated group were similar to those of the control. A significant increase in the serum levels of alanine aminotransferase and a discrete leukocyte infiltration with cytoplasmic vacuolization of the hepatocytes in the liver were detected in LE-treated animals. The preparations were not genotoxic or mutagenic. This study shows that LE and PRF are not antinutritional agents and presented low acute toxicity and no genotoxicity or mutagenicity. The present study contributes to the determination of the safety of using M. oleifera leaf proteins.","PeriodicalId":509865,"journal":{"name":"Pharmaceuticals","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of Cytotoxicity and Acute Oral Toxicity of Saline Extract and Protein-Rich Fraction from Moringa oleifera Lam. Leaves\",\"authors\":\"Robson Raion de Vasconcelos Alves, Alisson Macário de Oliveira, Gabryella Borges dos Prazeres, Abdênego Rodrigues da Silva, Franciele Florencio Costa, B. R. S. Barros, T. G. Souza, L. Coelho, C. D. de Melo, Magda RA Ferreira, L. Soares, C. Chagas, Maria Lígia Rodrigues Macedo, T. Napoleão, Mariana Pinheiro Fernandes, P. Paiva\",\"doi\":\"10.3390/ph17081045\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Moringa oleifera Lam. (horseradish tree) leaves demonstrate high nutritional value, are rich in proteins, and are widely used in folk medicine and food. This study investigated the presence of secondary metabolites and antinutritional proteins in leaf extract (LE) and the protein-rich fraction (PRF) derived from M. oleifera leaves, as well as the cytotoxicity to human cells, hemolytic activity, and in vivo acute toxicity and genotoxicity in mice. The flavonoids rutin and vitexin as well as trypsin inhibitors and lectins were detected in LE and PRF. Neither sample demonstrated toxicity against human peripheral blood mononuclear cells and both showed low hemolytic action. In vivo, LE and PRF did not show antinutritional effects and caused no death. The hematological parameters of the animals in the treated group were similar to those of the control. A significant increase in the serum levels of alanine aminotransferase and a discrete leukocyte infiltration with cytoplasmic vacuolization of the hepatocytes in the liver were detected in LE-treated animals. The preparations were not genotoxic or mutagenic. This study shows that LE and PRF are not antinutritional agents and presented low acute toxicity and no genotoxicity or mutagenicity. The present study contributes to the determination of the safety of using M. oleifera leaf proteins.\",\"PeriodicalId\":509865,\"journal\":{\"name\":\"Pharmaceuticals\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmaceuticals\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/ph17081045\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmaceuticals","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/ph17081045","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

辣木(Moringa oleifera Lam.(辣木)叶片具有很高的营养价值,富含蛋白质,被广泛用于民间医药和食品中。本研究调查了油麻叶提取物(LE)和富含蛋白质的馏分(PRF)中的次生代谢物和抗营养蛋白,以及对人体细胞的细胞毒性、溶血活性、小鼠体内急性毒性和遗传毒性。在油橄榄叶和油橄榄果中检测到了黄酮类化合物芦丁和牡荆素以及胰蛋白酶抑制剂和凝集素。两种样品都没有显示出对人类外周血单核细胞的毒性,而且都显示出较低的溶血作用。在体内,LE 和 PRF 没有显示出抗营养作用,也没有造成死亡。处理组动物的血液学参数与对照组相似。经 LE 处理的动物血清中的丙氨酸氨基转移酶水平明显升高,肝脏中的肝细胞出现离散的白细胞浸润和胞浆空泡化。这些制剂没有遗传毒性或诱变性。这项研究表明,LE 和 PRF 不是抗营养剂,而且急性毒性低,没有遗传毒性和致突变性。本研究有助于确定使用油橄榄叶蛋白的安全性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of Cytotoxicity and Acute Oral Toxicity of Saline Extract and Protein-Rich Fraction from Moringa oleifera Lam. Leaves
Moringa oleifera Lam. (horseradish tree) leaves demonstrate high nutritional value, are rich in proteins, and are widely used in folk medicine and food. This study investigated the presence of secondary metabolites and antinutritional proteins in leaf extract (LE) and the protein-rich fraction (PRF) derived from M. oleifera leaves, as well as the cytotoxicity to human cells, hemolytic activity, and in vivo acute toxicity and genotoxicity in mice. The flavonoids rutin and vitexin as well as trypsin inhibitors and lectins were detected in LE and PRF. Neither sample demonstrated toxicity against human peripheral blood mononuclear cells and both showed low hemolytic action. In vivo, LE and PRF did not show antinutritional effects and caused no death. The hematological parameters of the animals in the treated group were similar to those of the control. A significant increase in the serum levels of alanine aminotransferase and a discrete leukocyte infiltration with cytoplasmic vacuolization of the hepatocytes in the liver were detected in LE-treated animals. The preparations were not genotoxic or mutagenic. This study shows that LE and PRF are not antinutritional agents and presented low acute toxicity and no genotoxicity or mutagenicity. The present study contributes to the determination of the safety of using M. oleifera leaf proteins.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信