免疫调节细胞和细胞因子可区分类风湿关节炎非高疾病活动度患者的疾病活动度评分 28-Remission 状态和超声分级

IF 4.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
L. Liou, Yao-Fan Fang, P. Tsai, Yen-Fu Chen, Che-Tzu Chang, Chih-chieh Chen, W. Chiang
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引用次数: 0

摘要

目前尚不清楚免疫调节细胞因子和细胞是否与疾病活动度评分28(DAS28)得分和超声波等级/分数相关。在此,我们研究了类风湿性关节炎(RA)患者的免疫调节细胞因子或细胞与不同 DAS28 评分或超声分级/分数之间的关系。这项研究共招募了 50 名类风湿关节炎患者(共就诊 147 次),他们在基线时的 DAS28-ESR 评分为缓解/低度/中度(92% 为缓解,疾病活动度低)。一年内采集血液并进行三次超声波检查。检查血液中调节性B细胞和T调节性1型细胞的百分比以及M2巨噬细胞的数量。使用 ELISA 方法评估了血浆中 10 种免疫调节细胞因子 IL-4、IL-5、IL-9、IL-10、IL-13、IL-27、IL-35、TGF-β1、sTNF-R1 和 sTNF-R2 以及单核细胞趋化蛋白-1(MCP-1)的水平。研究了细胞因子和细胞与不同 DAS28 评分和超声分级的相关性,并对不同类别 DAS28 评分和超声分级的细胞因子和细胞进行了比较。DAS28-ESR < 2.6(缓解)亚组的血浆 TGF-β1 水平高于 DAS28-ESR ≥ 2.6(未缓解)亚组(p = 0.037)。然而,高超声分级亚组的血浆 TGF-β1 水平高于低超声分级亚组(p = 0.007)。DAS28-MCP-1 < 2.2 亚组的 M2 巨噬细胞数量低于 DAS28-MCP-1 ≥ 2.2 亚组(p = 0.036)。超声分级高的患者 TGF-β1、sTNF-R2、IL-10 和 IL-27 水平高于超声分级低的患者。在未缓解的 DAS28-ESR 亚组中,IL-27 也高于缓解亚组(P = 0.025)。此外,2011 年美国风湿病学会/欧洲抗风湿病联盟(ACR/EULAR)缓解亚组的 sTNF-R1 水平明显低于 2011 年 ACR/EULAR 非缓解亚组(p = 0.007)。这一趋势反映在较低的 sTNF-R1 水平与较低的 DAS28-MCP-1 评分相关(rho = 0.222,p = 0.007)。我们的结论是,血浆 TGF-β1 水平高表明 DAS28-ESR 缓解(<2.6)亚组和超声分级高亚组。IL-27 可能将未缓解的 DAS28-ESR 与高超声分级联系起来。低 sTNF-R1 水平可能将低 DAS28-MCP-1 评分与 2011 ACR/EULAR 缓解亚组联系起来。这表明,DAS28评分和超声分级之间存在不一致的免疫炎症异常,而且不同的DAS28公式类别之间也存在差异。因此,本研究可为进一步研究每种 DAS28 公式和超声分级/评分背后的单个细胞因子和免疫调节细胞提供依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunoregulatory Cells and Cytokines Discriminate Disease Activity Score 28-Remission Statuses and Ultrasound Grades in Rheumatoid Arthritis Patients with Non-High Disease Activity
It is not clear whether immunoregulatory cytokines and cells are associated with Disease Activity Score 28 (DAS28) scores and ultrasound grades/scores. Here, we investigated the relationships between immunoregulatory cytokines or cells and different DAS28 scores or ultrasound grades/scores in patients with rheumatoid arthritis (RA). This study enrolled 50 RA patients (with 147 visits) who had remission/low/moderate DAS28-ESR scores (92% in remission and low disease activity) at baseline. Blood was collected and an ultrasound was performed three times in a year. Percentages of regulatory B cells and T regulatory type 1 cells and M2 macrophage numbers in the blood were examined. Plasma levels of 10 immunoregulatory cytokines IL-4, IL-5, IL-9, IL-10, IL-13, IL-27, IL-35, TGF-β1, sTNF-R1, and sTNF-R2 and monocyte chemotactic protein-1 (MCP-1) were assessed using ELISA assay. The correlations of cytokines and cells with different DAS28 scores and ultrasound grades were investigated, and cytokines and cells were compared between different categories of DAS28 scores and ultrasound grades. Plasma TGF-β1 levels were higher in the DAS28-ESR < 2.6 (remission) subgroup than in the DAS28-ESR ≥ 2.6 (nonremission) subgroup (p = 0.037). However, plasma TGF-β1 levels were higher in the high ultrasound grade subgroup than those in the low ultrasound grade subgroup (p = 0.007). The number of M2 macrophages was lower in the DAS28-MCP-1 < 2.2 subgroup than in the DAS28-MCP-1 ≥ 2.2 subgroup (p = 0.036). The levels of TGF-β1, sTNF-R2, IL-10, and IL-27 were higher in patients with high ultrasound grades than in those with low ultrasound grades. IL-27 was also higher in the nonremission DAS28-ESR subgroup than the remission one (p = 0.025). Moreover, sTNF-R1 levels in the 2011 American College of Rheumatology/European League Against Rheumatism (ACR/EULAR) remission subgroup were significantly lower than in the 2011 ACR/EULAR nonremission subgroup (p = 0.007). This trend was reflected in that lower sTNF-R1 levels correlated with low DAS28-MCP-1 scores (rho = 0.222, p = 0.007). We conclude that high plasma TGF-β1 levels indicate the DAS28-ESR remission (<2.6) subgroup and the high ultrasound grade subgroup. IL-27 probably connects the nonremission DAS28-ESR to high ultrasound grades. Low sTNF-R1 levels probably link low DAS28-MCP-1 scores with the 2011 ACR/EULAR remission subgroup. It suggests that incongruent immuno-inflammatory abnormalities exist between DAS28 scores and ultrasound grades, and are also dissimilar among various DAS28-formula categories. Therefore, this study may provide a basis for further research into individual cytokines and immunoregulatory cells behind each DAS28 formula and ultrasound grades/scores.
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来源期刊
International Journal of Molecular Sciences
International Journal of Molecular Sciences Chemistry-Organic Chemistry
CiteScore
8.10
自引率
10.70%
发文量
13472
审稿时长
17.49 days
期刊介绍: The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).
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