瘦素受体不同变体在人类肾上腺肿瘤类型中的作用

IF 4.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Molecular Sciences Pub Date : 2024-08-09 DOI:10.3390/ijms25168682

Anna Klimont, M. Ruciński, N. Sawicka-Gutaj, Marta Szyszka, Małgorzata Blatkiewicz, Tomasz Wierzbicki, Marek Karczewski, M. Janicka-Jedyńska, M. Ruchała, Hanna Komarowska

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引用次数: 0

摘要

该研究旨在评估肾上腺肿瘤中瘦素受体同工酶的诊断和预后意义。在一项单中心研究中，96名患者（19名肾上腺皮质癌患者和77名良性肿瘤患者）接受了肾上腺切除术。共有 14 个来自肾脏捐献者的未受影响的肾上腺组织作为对照。研究人员采集了空腹血样进行化验，并通过 RT-qPCR 评估了瘦素受体同工酶的 mRNA 表达。研究分析了mRNA表达与临床数据之间的相关性，并通过实时细胞分析仪测量了NCI-H295R细胞的增殖情况。所有肾上腺病变均表达瘦素受体同工酶。在癌组织中观察到的LepR1表达明显低于腺瘤和对照组（p = 0.016）。LepR3和LepR6的表达与总生存率相关（p = 0.036），而LepR2和LepR4以及LepR5的表达与早晨血清皮质醇水平成反比（p = 0.041）。瘦素可减少NCI-H295R细胞的增殖（p < 0.0001）。该研究强调了瘦素受体同工酶在肾上腺肿瘤中的诊断和预后意义。具体来说，LepR1可作为癌变的诊断标志物，而LepR3和LepR6则可作为预后标志物。

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Role of Different Variants of Leptin Receptor in Human Adrenal Tumor Types

The aim of the study was to evaluate the diagnostic and prognostic significance of leptin receptor isoforms in adrenal tumors. In a single-center study, 96 patients (19 with adrenal cortical carcinoma and 77 with benign tumors) underwent an adrenalectomy. A total of 14 unaffected adrenal gland tissues from kidney donors were used as controls. Fasting blood samples were collected for laboratory tests, and mRNA expressions of leptin receptor isoforms were assessed by RT-qPCR. The study analyzed correlations between mRNA expressions and clinical data and measured NCI-H295R cell proliferation via a real-time cell analyzer. All adrenal lesions expressed leptin receptor isoforms. Significantly lower LepR1 expression was observed in carcinoma tissues than in adenomas and controls (p = 0.016). Expressions of LepR3&LepR6 were correlated with overall survival (p = 0.036), while LepR2&LepR4 and LepR5 expressions were inversely related to morning serum cortisol levels (p = 0.041). Leptin reduced NCI-H295R cell proliferation (p < 0.0001). The study highlights the diagnostic and prognostic significance of leptin receptor isoforms in adrenal tumors. Specifically, LepR1 may serve as a diagnostic marker for carcinomas, while LepR3&LepR6 have potential use as prognostic markers.

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来源期刊

International Journal of Molecular Sciences Chemistry-Organic Chemistry

CiteScore

8.10

自引率

10.70%

发文量

13472

审稿时长

17.49 days

期刊介绍： The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).