实施独特的空间蛋白质基因组技术;机遇、挑战和主要考虑因素。

IF 3.4 3区 医学 Q3 IMMUNOLOGY
Bram Verstappe, Charlotte L Scott
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引用次数: 0

摘要

近年来,随着蛋白质基因组学技术(包括空间分析技术)的显著进步,我们了解组织细胞复杂性的能力发生了革命性的变化。人类细胞图谱(HCA)计划旨在为人体健康和患病情况下的所有细胞绘制图谱。有了这些信息,就能确定疾病的新型生物标志物,当然还能找到治疗途径。然而,在生成任何特定健康或疾病组织的图谱之前,应考虑几个因素,包括哪些特定技术是解决当前生物学问题的最佳方法。在这篇综述中,我们将重点介绍我们认为在实验设计和分析过程中非常重要的一些注意事项,目的是帮助大家驾驭瞬息万变的可用技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Implementing distinct spatial proteogenomic technologies: opportunities, challenges, and key considerations.

Our ability to understand the cellular complexity of tissues has been revolutionized in recent years with significant advances in proteogenomic technologies including those enabling spatial analyses. This has led to numerous consortium efforts, such as the human cell atlas initiative which aims to profile all cells in the human body in healthy and diseased contexts. The availability of such information will subsequently lead to the identification of novel biomarkers of disease and of course therapeutic avenues. However, before such an atlas of any given healthy or diseased tissue can be generated, several factors should be considered including which specific techniques are optimal for the biological question at hand. In this review, we aim to highlight some of the considerations we believe to be important in the experimental design and analysis process, with the goal of helping to navigate the rapidly changing landscape of technologies available.

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来源期刊
CiteScore
8.40
自引率
2.20%
发文量
101
审稿时长
3-8 weeks
期刊介绍: Clinical & Experimental Immunology (established in 1966) is an authoritative international journal publishing high-quality research studies in translational and clinical immunology that have the potential to transform our understanding of the immunopathology of human disease and/or change clinical practice. The journal is focused on translational and clinical immunology and is among the foremost journals in this field, attracting high-quality papers from across the world. Translation is viewed as a process of applying ideas, insights and discoveries generated through scientific studies to the treatment, prevention or diagnosis of human disease. Clinical immunology has evolved as a field to encompass the application of state-of-the-art technologies such as next-generation sequencing, metagenomics and high-dimensional phenotyping to understand mechanisms that govern the outcomes of clinical trials.
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