利用真空-紫外光圆二色光谱观测 FUS-LC 的液-液相分离。

IF 2.8 4区化学 Q2 CHEMISTRY, ANALYTICAL

Chirality Pub Date : 2024-08-11 DOI:10.1002/chir.23707

Kentaro Fujii, Yudai Izumi, Nobuo Maita, Koichi Matsuo, Masato Kato

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引用次数: 0

摘要

为了揭示融合肉瘤蛋白（FUS-LC）的低复杂性结构域介导液-液相分离（LLPS）的结构机制，我们进行了一项真空紫外圆二色性光谱（VUV-CD）研究，这是一种分析蛋白质二级结构的技术。VUV-CD 测量是在日本广岛同步辐射中心（HiSOR）的 BL12 VUV-CD 站进行的。在将样品温度控制在 37°C 至 5°C 之间的同时，在 180 至 260 nm 波长范围内测量了 CD 光谱，以获得 FUS-LC 的 LLPS。在 37°C 时获得的 CD 光谱在 195nm 处显示出一个较大的负峰，在 220nm 附近显示出一个较小的负肩。随着样品温度的降低，195 nm 附近的峰强度减小。光谱变化源于 LLPS 的形成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Observation of the Liquid–Liquid Phase Separation of FUS-LC Using Vacuum-Ultraviolet Circular Dichroism Spectroscopy

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Observation of the Liquid–Liquid Phase Separation of FUS-LC Using Vacuum-Ultraviolet Circular Dichroism Spectroscopy

To reveal the structural mechanism by which the low-complexity domain of the fused in sarcoma protein (FUS-LC) mediates liquid–liquid phase separation (LLPS), we conducted a vacuum-ultraviolet circular dichroism (VUV-CD) spectroscopic study, a technique to analyze the secondary structures of proteins. The VUV-CD measurements were performed at the BL12 VUV-CD station at the Hiroshima Synchrotron Radiation Center (HiSOR) in Japan. CD spectra were measured between 180 and 260 nm while controlling the temperature of samples from 37°C to 5°C to obtain the LLPS of FUS-LC. The CD spectrum obtained at 37°C exhibited a large negative peak at 195 nm and a small negative shoulder near 220 nm. The peak intensity around 195 nm decreased as the sample temperature decreased. The spectral changes originated from the LLPS formation.

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来源期刊

Chirality 医学-分析化学

CiteScore

4.40

自引率

5.00%

发文量

124

审稿时长

1 months

期刊介绍： The main aim of the journal is to publish original contributions of scientific work on the role of chirality in chemistry and biochemistry in respect to biological, chemical, materials, pharmacological, spectroscopic and physical properties. Papers on the chemistry (physiochemical, preparative synthetic, and analytical), physics, pharmacology, clinical pharmacology, toxicology, and other biological aspects of chiral molecules will be published.