如何从真实人体毛发中追踪非法电子烟中的依托咪酯:识别、定量和多因素分析。

IF 2.8 4区 医学 Q2 TOXICOLOGY
Wanting Xie, Liying Zhou, Jinting Liu, Ziyi Li, Zehong Li, Wen Gao, Yan Shi
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引用次数: 0

摘要

目的:滥用含有依托咪酯(ET)的非法电子烟会对公众的身心健康产生重大影响。本研究旨在利用超高效液相色谱/串联质谱法(UHPLC-MS/MS)建立一种快速定量方法,用于有针对性地筛查头发样本中的依托咪酯 (ET) 及其代谢物依托咪酯酸 (ETA):在 20 毫克毛发中加入 1 毫升含有内标 ET-d5 的甲醇溶液(浓度为 50 pg/mg),并在 4 °C 以下研磨。离心后,将 5 μL 上清液注入 UHPLC-MS/MS 系统:结果:ET 的检出限(LOD)和定量限(LOQ)分别为 1 pg/mg 和 10 pg/mg,ETA 的检出限(LOD)和定量限(LOQ)分别为 10 pg/mg 和 25 pg/mg。所有分析物的校准曲线均显示出良好的线性关系(r > 0.997),表明该方法可靠。准确度在 92.12% 至 110.72% 之间。在所有浓度水平下,所有分析物的日内和日间精密度均低于 10.13%。分析物的回收率为 86.90% 至 101.43%,基质效应为 -18.55% 至 -14.93%:经过验证的方法成功地用于分析了 105 份疑似 ET 使用者的头发样本。其中,50 份检测结果呈 ET 阳性,43 份检测结果呈 ETA 阳性,均高于 LOQ。这表明所开发的超高效液相色谱-质谱/质谱法能有效检测头发样本中的 ET 和 ETA,有助于解决非法电子烟滥用问题及其对公众健康的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

How to trace etomidate in illegal E-cigarettes from authentic human hair: identification, quantification and multiple-factor analysis.

How to trace etomidate in illegal E-cigarettes from authentic human hair: identification, quantification and multiple-factor analysis.

Purpose: The abusive consumption of illegal E-cigarettes containing etomidate (ET) can have a significant impact on public mental and physical well-being. The purpose of this study is to establish a rapid quantitative method using ultra-high-performance liquid chromatography/tandem mass spectrometry (UHPLC-MS/MS) for the targeted screening of etomidate (ET) and its metabolite etomidate acid (ETA) in hair samples.

Methods: A 1 mL methanol solution containing the internal standard ET-d5 at a concentration of 50 pg/mg was added to 20 mg of hair and milled below 4 °C. After centrifugation, 5 μL of the supernatant was injected into a UHPLC-MS/MS system.

Results: The limit of detection (LOD) and limit of quantification (LOQ) were determined to be 1 pg/mg and 10 pg/mg, respectively, for ET, and 10 pg/mg and 25 pg/mg, respectively, for ETA. Calibration curves for all analytes showed good linearity (r > 0.997), indicating a reliable method. Accuracies were between 92.12% and 110.72%. Intra-day and inter-day precision for all analytes at all concentration levels were below 10.13%. Analyte recoveries ranged from 86.90% to 101.43%, with a matrix effect ranging from -18.55% to -14.93%.

Conclusions: The validated method was successfully used to analyze 105 hair samples from suspected ET users. Of these, 50 tested positive for ET and 43 tested positive for ETA above the LOQ. This demonstrates the effectiveness of the developed UHPLC-MS/MS method in detecting ET and ETA in hair samples, which could be instrumental in addressing the issue of illegal E-cigarette abuse and its impact on public health.

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来源期刊
Forensic Toxicology
Forensic Toxicology TOXICOLOGY-
CiteScore
5.80
自引率
9.10%
发文量
40
审稿时长
3 months
期刊介绍: The journal Forensic Toxicology provides an international forum for publication of studies on toxic substances, drugs of abuse, doping agents, chemical warfare agents, and their metabolisms and analyses, which are related to laws and ethics. It includes original articles, reviews, mini-reviews, short communications, and case reports. Although a major focus of the journal is on the development or improvement of analytical methods for the above-mentioned chemicals in human matrices, appropriate studies with animal experiments are also published. Forensic Toxicology is the official publication of the Japanese Association of Forensic Toxicology (JAFT) and is the continuation of the Japanese Journal of Forensic Toxicology (ISSN 0915-9606).
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