基质辅助激光解吸电离飞行时间质谱结合光密度比快速检测耐头孢曲松沙门氏菌。

IF 4.6 2区 医学 Q1 MICROBIOLOGY
Chao Fang, Zheng Zhou, Mingming Zhou, Jianping Li
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引用次数: 0

摘要

背景:沙门氏菌对以头孢曲松(CRO)为代表的第三代头孢菌素的耐药率增加,可能会导致经验性使用第三代头孢菌素治疗儿童沙门氏菌感染的失败。本研究旨在评估一种快速检测耐 CRO 沙门氏菌(CRS)的新方法:我们引入了有无 CRO 的光密度比(ROD)概念,并将其与基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)相结合,建立了快速检测 CRS 的新方案:结果:通过模式菌株试验确定的最佳培养时间和 CRO 浓度分别为 2 小时和 8 µg/ml。随后,我们对 120 株临床菌株进行了确证试验。根据接收者操作特征曲线分析,区分 CRS 和非 CRS 菌株的 ROD 临界值为 0.818 [曲线下面积:1.000;95% 置信区间:1.000;95% 置信区间:1.000]:1.000;95% 置信区间:0.970-1.000;灵敏度:100.00%;特异度:100%;P - 3]:总之,ROD 和 MALDI-TOF MS 组合方案是一种快速、准确、经济的 CRS 检测方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rapid detection of ceftriaxone-resistant Salmonella by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry combined with the ratio of optical density.

Background: The increased resistance rate of Salmonella to third-generation cephalosporins represented by ceftriaxone (CRO) may result in the failure of the empirical use of third-generation cephalosporins for the treatment of Salmonella infection in children. The present study was conducted to evaluate a novel method for the rapid detection of CRO-resistant Salmonella (CRS).

Methods: We introduced the concept of the ratio of optical density (ROD) with and without CRO and combined it with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) to establish a new protocol for the rapid detection of CRS.

Results: The optimal incubation time and CRO concentration determined by the model strain test were 2 h and 8 µg/ml, respectively. We then conducted confirmatory tests on 120 clinical strains. According to the receiver operating characteristic curve analysis, the ROD cutoff value for distinguishing CRS and non-CRS strains was 0.818 [area under the curve: 1.000; 95% confidence interval: 0.970-1.000; sensitivity: 100.00%; specificity: 100%; P < 10- 3].

Conclusions: In conclusion, the protocol for the combined ROD and MALDI-TOF MS represents a rapid, accurate, and economical method for the detection of CRS.

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来源期刊
CiteScore
8.60
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.
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