激活 G 蛋白偶联磺胺嘧啶受体可抑制埃及伊蚊的血餐摄入。

IF 4.4 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Linlong Jiang, Xiao Bing Xie, Lei Zhang, Yu Tang, Xiaojing Zhu, Yuqi Huang, Yue Hong, Bill S. Hansson, Zong Jie Cui, Qian Han
{"title":"激活 G 蛋白偶联磺胺嘧啶受体可抑制埃及伊蚊的血餐摄入。","authors":"Linlong Jiang,&nbsp;Xiao Bing Xie,&nbsp;Lei Zhang,&nbsp;Yu Tang,&nbsp;Xiaojing Zhu,&nbsp;Yuqi Huang,&nbsp;Yue Hong,&nbsp;Bill S. Hansson,&nbsp;Zong Jie Cui,&nbsp;Qian Han","doi":"10.1096/fj.202401165R","DOIUrl":null,"url":null,"abstract":"<p>Little is known about the blood-feeding physiology of arbovirus vector <i>Aedes aegypti</i> although this type of mosquito is known to transmit infectious diseases dengue, Zika, yellow fever, and chikungunya. Blood feeding in the female <i>A. aegypti</i> mosquito is essential for egg maturation and for transmission of disease agents between human subjects. Here, we identify the <i>A. aegypti</i> sulfakinin receptor gene <i>SKR</i> from the <i>A. aegypti</i> genome and show that <i>SKR</i> is expressed at different developmental stages and in varied anatomical localizations in the adult mosquito (at three days after eclosion), with particularly high expression in the CNS. Knockingdown sulfakinin and sulfakinin receptor gene expression in the female <i>A. aegypti</i> results in increased blood meal intake, but microinjection in the thorax of the sulfakinin peptide 1 and 2 both inhibits dose dependently blood meal intake (and delays the time course of blood intake), which is reversible with receptor antagonist. Sulfakinin receptor expressed ectopically in mammalian cells CHO-K1 responds to sulfakinin stimulation with persistent calcium spikes, blockable with receptor antagonist. These data together suggest that activation of the Gq protein-coupled (i.e., calcium-mobilizing) sulfakinin receptor inhibits blood meal intake in female <i>A. aegypti</i> mosquitoes and could serve as a strategic node for the future control of <i>A. aegypti</i> mosquito reproduction/population and disease transmission.</p>","PeriodicalId":50455,"journal":{"name":"FASEB Journal","volume":null,"pages":null},"PeriodicalIF":4.4000,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fj.202401165R","citationCount":"0","resultStr":"{\"title\":\"Activation of the G protein-coupled sulfakinin receptor inhibits blood meal intake in the mosquito Aedes aegypti\",\"authors\":\"Linlong Jiang,&nbsp;Xiao Bing Xie,&nbsp;Lei Zhang,&nbsp;Yu Tang,&nbsp;Xiaojing Zhu,&nbsp;Yuqi Huang,&nbsp;Yue Hong,&nbsp;Bill S. Hansson,&nbsp;Zong Jie Cui,&nbsp;Qian Han\",\"doi\":\"10.1096/fj.202401165R\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Little is known about the blood-feeding physiology of arbovirus vector <i>Aedes aegypti</i> although this type of mosquito is known to transmit infectious diseases dengue, Zika, yellow fever, and chikungunya. Blood feeding in the female <i>A. aegypti</i> mosquito is essential for egg maturation and for transmission of disease agents between human subjects. Here, we identify the <i>A. aegypti</i> sulfakinin receptor gene <i>SKR</i> from the <i>A. aegypti</i> genome and show that <i>SKR</i> is expressed at different developmental stages and in varied anatomical localizations in the adult mosquito (at three days after eclosion), with particularly high expression in the CNS. Knockingdown sulfakinin and sulfakinin receptor gene expression in the female <i>A. aegypti</i> results in increased blood meal intake, but microinjection in the thorax of the sulfakinin peptide 1 and 2 both inhibits dose dependently blood meal intake (and delays the time course of blood intake), which is reversible with receptor antagonist. Sulfakinin receptor expressed ectopically in mammalian cells CHO-K1 responds to sulfakinin stimulation with persistent calcium spikes, blockable with receptor antagonist. These data together suggest that activation of the Gq protein-coupled (i.e., calcium-mobilizing) sulfakinin receptor inhibits blood meal intake in female <i>A. aegypti</i> mosquitoes and could serve as a strategic node for the future control of <i>A. aegypti</i> mosquito reproduction/population and disease transmission.</p>\",\"PeriodicalId\":50455,\"journal\":{\"name\":\"FASEB Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2024-08-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fj.202401165R\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"FASEB Journal\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1096/fj.202401165R\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"FASEB Journal","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1096/fj.202401165R","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

尽管已知埃及伊蚊可传播登革热、寨卡病毒、黄热病和基孔肯雅热等传染病,但人们对这种虫媒病毒病媒的吸血生理学知之甚少。雌性埃及伊蚊的采血对于卵的成熟和疾病病原体在人与人之间的传播至关重要。在这里,我们从埃及姬蚊基因组中鉴定出了埃及姬蚊磺胺肽受体基因 SKR,并表明 SKR 在成蚊的不同发育阶段和不同的解剖定位中都有表达(在羽化后三天),尤其是在中枢神经系统中表达较高。在雌性埃及蚊中敲除磺胺肽和磺胺肽受体基因表达会导致血餐摄入量增加,但在胸部微量注射磺胺肽 1 和 2 都会抑制血餐摄入量(并延缓血餐摄入量的时间进程),受体拮抗剂可逆转这种抑制作用。在哺乳动物细胞 CHO-K1 中异位表达的磺胺肽受体对磺胺肽刺激的反应是持续的钙尖峰,受体拮抗剂可阻断这种反应。这些数据共同表明,激活 Gq 蛋白偶联(即钙动员)的磺胺嘧啶受体可抑制埃及雌蚊的血餐摄入,并可作为未来控制埃及雌蚊繁殖/种群和疾病传播的战略节点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Activation of the G protein-coupled sulfakinin receptor inhibits blood meal intake in the mosquito Aedes aegypti

Activation of the G protein-coupled sulfakinin receptor inhibits blood meal intake in the mosquito Aedes aegypti

Little is known about the blood-feeding physiology of arbovirus vector Aedes aegypti although this type of mosquito is known to transmit infectious diseases dengue, Zika, yellow fever, and chikungunya. Blood feeding in the female A. aegypti mosquito is essential for egg maturation and for transmission of disease agents between human subjects. Here, we identify the A. aegypti sulfakinin receptor gene SKR from the A. aegypti genome and show that SKR is expressed at different developmental stages and in varied anatomical localizations in the adult mosquito (at three days after eclosion), with particularly high expression in the CNS. Knockingdown sulfakinin and sulfakinin receptor gene expression in the female A. aegypti results in increased blood meal intake, but microinjection in the thorax of the sulfakinin peptide 1 and 2 both inhibits dose dependently blood meal intake (and delays the time course of blood intake), which is reversible with receptor antagonist. Sulfakinin receptor expressed ectopically in mammalian cells CHO-K1 responds to sulfakinin stimulation with persistent calcium spikes, blockable with receptor antagonist. These data together suggest that activation of the Gq protein-coupled (i.e., calcium-mobilizing) sulfakinin receptor inhibits blood meal intake in female A. aegypti mosquitoes and could serve as a strategic node for the future control of A. aegypti mosquito reproduction/population and disease transmission.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
FASEB Journal
FASEB Journal 生物-生化与分子生物学
CiteScore
9.20
自引率
2.10%
发文量
6243
审稿时长
3 months
期刊介绍: The FASEB Journal publishes international, transdisciplinary research covering all fields of biology at every level of organization: atomic, molecular, cell, tissue, organ, organismic and population. While the journal strives to include research that cuts across the biological sciences, it also considers submissions that lie within one field, but may have implications for other fields as well. The journal seeks to publish basic and translational research, but also welcomes reports of pre-clinical and early clinical research. In addition to research, review, and hypothesis submissions, The FASEB Journal also seeks perspectives, commentaries, book reviews, and similar content related to the life sciences in its Up Front section.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信