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引用次数: 0
摘要
非洲猪瘟病毒（ASFV）的出现和传播对全球养猪业构成了重大威胁，需要采用创新方法来遏制和控制病毒。最近的一项研究（Z. Zheng, L. Xu, H. Dou, Y. Zhou, X., et al., Microbiol Spectr 12: e02164-23, 2024, https://doi.org/10.1128/spectrum.02164-23）建立了针对非洲猪瘟病毒基因组的多重CRISPR-Cas系统，并在体外和体内测试了其抗病毒活性。应用该系统后，体外病毒复制明显减少，而表达该系统的种系编辑猪在持续表达引导 RNA 的情况下表现出正常生长。虽然与非工程猪相比，在ASFV挑战下没有观察到存活优势，但这标志着利用种系编辑技术来寻求ASFV抗性的首次尝试，并为未来利用CRISPR-Cas技术进行抗病动物育种铺平了道路。
Multiplexed CRISPR-Cas system targeting ASFV genes in vivo: solution lies within.
The emergence and spread of the African swine fever virus (ASFV) posed a significant threat to the global swine breeding industry, calling for innovative approaches benefiting viral containment and control. A recent study (Z. Zheng, L. Xu, H. Dou, Y. Zhou, X., et al., Microbiol Spectr 12: e02164-23, 2024, https://doi.org/10.1128/spectrum.02164-23) established a multiplexed CRISPR-Cas system targeting the genome of ASFV and tested the consequent antiviral activity both in vitro and in vivo. Application of this system showed a significant reduction of viral replication in vitro, while the germline-edited pigs expressing this system exhibited normal growth with continuous guide RNA expression. Although no survival advantage was observed upon ASFV challenge compared with nonengineered pigs, this marks the first attempt of germline editing to pursue ASFV resistance and paves the way for future disease-resistant animal breeding approaches utilizing CRISPR-Cas technology.
期刊介绍：
Microbiology Spectrum publishes commissioned review articles on topics in microbiology representing ten content areas: Archaea; Food Microbiology; Bacterial Genetics, Cell Biology, and Physiology; Clinical Microbiology; Environmental Microbiology and Ecology; Eukaryotic Microbes; Genomics, Computational, and Synthetic Microbiology; Immunology; Pathogenesis; and Virology. Reviews are interrelated, with each review linking to other related content. A large board of Microbiology Spectrum editors aids in the development of topics for potential reviews and in the identification of an editor, or editors, who shepherd each collection.
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