透明质酸通过 CD44 和 TGF-β2 介导人晶状体上皮细胞的上皮-间质转化。

IF 1.1 4区 医学 Q3 OPHTHALMOLOGY
Arquivos brasileiros de oftalmologia Pub Date : 2024-08-02 eCollection Date: 2024-01-01 DOI:10.5935/0004-2749.2023-0163
Yumin Gui, Jianjun Peng, Shuanghong Jiang
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引用次数: 0

摘要

目的:人类晶状体上皮细胞的上皮-间充质转化在后囊不透明中起着作用,后囊不透明是一种纤维化过程,会导致一种常见的白内障。透明质酸与这种纤维化有关。研究调查了转化生长因子(TGF)-β2 在上皮-间质转化中的作用。然而,TGF-β2 在透明质酸介导的晶状体上皮细胞纤维化中的作用仍然未知。我们在此研究了TGF-β2在透明质酸介导的晶状体上皮细胞上皮-间质转化中的作用:方法:用 Lipofectamine 3000 转染试剂将 CD44-siRNA 感染培养的人晶状体上皮细胞(HLEB3)。用 CCK-8 试剂盒测定细胞活力,用划痕试验测定细胞迁移。细胞氧化应激用二氯二氢荧光素二乙酸酯测定法和流式细胞仪进行分析。通过免疫组化染色检测了HLEB3细胞中的TGF-β2水平。mRNA表达水平通过实时定量聚合酶链反应测定:结果:透明质酸处理(1.0 μM,24 h)增加了 HLEB3 细胞的上皮-间质转化。TGF-β2 水平的增加与培养液中 CD44 水平的增加相对应。然而,阻断CD44功能可显著降低TGF-β2介导的HLEB3细胞上皮-间质转化反应:我们的研究表明,CD44和TGF-β2都是透明质酸介导的晶状体上皮-间充质转化的关键因素,而TGF-β2在上皮-间充质转化中的作用受CD44调控。这些结果表明,CD44可作为预防透明质酸诱导的后囊不透明的靶点。我们的研究结果表明,CD44/TGF-β2对透明质酸诱导的晶状体上皮细胞上皮-间质转化至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hyaluronic acid-mediated epithelial-mesenchymal transition of human lens epithelial cells via CD44 and TGF-β2.

Purpose: The epithelial-mesenchymal transition of human lens epithelial cells plays a role in posterior capsule opacification, a fibrotic process that leads to a common type of cataract. Hyaluronic acid has been implicated in this fibrosis. Studies have investigated the role of transforming growth factor (TGF)-β2 in epithelial-mesenchymal transition. However, the role of TGF-β2 in hyaluronic acid-mediated fibrosis of lens epithelial cell remains unknown. We here examined the role of TGF-β2 in the hyaluronic acid-mediated epithelial-mesenchymal transition of lens epithelial cells.

Methods: Cultured human lens epithelial cells (HLEB3) were infected with CD44-siRNA by using the Lipofectamine 3000 transfection reagent. The CCK-8 kit was used to measure cell viability, and the scratch assay was used to determine cell migration. Cell oxidative stress was analyzed in a dichloro-dihydro-fluorescein diacetate assay and by using a flow cytometer. The TGF-β2 level in HLEB3 cells was examined through immunohistochemical staining. The TGF-β2 protein level was determined through western blotting. mRNA expression levels were determined through quantitative real-time polymerase chain reaction.

Results: Treatment with hyaluronic acid (1.0 μM, 24 h) increased the epithelial-mesenchymal transition of HLEB3 cells. The increase in TGF-β2 levels corresponded to an increase in CD44 levels in the culture medium. However, blocking the CD44 function significantly reduced the TGF-β2-mediated epithelial-mesenchymal transition response of HLEB3 cells.

Conclusions: Our study showed that both CD44 and TGF-β2 are critical contributors to the hyaluronic acid-mediated epithelial-mesenchymal transition of lens epithelial cells, and that TGF-β2 in epithelial-mesenchymal transition is regulated by CD44. These results suggest that CD44 could be used as a target for preventing hyaluronic acid-induced posterior capsule opacification. Our findings suggest that CD44/TGF-β2 is crucial for the hyaluronic acid-induced epithelial-mesenchymal transition of lens epithelial cells.

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来源期刊
CiteScore
1.60
自引率
0.00%
发文量
200
审稿时长
6-12 weeks
期刊介绍: The ABO-ARQUIVOS BRASILEIROS DE OFTALMOLOGIA (ABO, ISSN 0004-2749 - print and ISSN 1678-2925 - (ABO, ISSN 0004-2749 - print and ISSN 1678-2925 - electronic version), the official bimonthly publication of the Brazilian Council of Ophthalmology (CBO), aims to disseminate scientific studies in Ophthalmology, Visual Science and Health public, by promoting research, improvement and updating of professionals related to the field.
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