Wei Wang, Jiahui Weng, Jie Wei, Qinghuan Zhang, Yu Zhou, Yanju He, Limei Zhang, Wenting Li, Yi Zhang, Zhiren Zhang, Xiaobin Li
{"title":"通过全基因组测序深入了解耐碳青霉烯类和耐多药鲍曼不动杆菌染色体携带的 blaOXA-23。","authors":"Wei Wang, Jiahui Weng, Jie Wei, Qinghuan Zhang, Yu Zhou, Yanju He, Limei Zhang, Wenting Li, Yi Zhang, Zhiren Zhang, Xiaobin Li","doi":"10.1128/spectrum.00501-24","DOIUrl":null,"url":null,"abstract":"<p><p>Carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) poses a significant threat to hospitalized patients as effective therapeutic options are scarce. Based on the genomic characteristics of the CRAB strain AB2877 harboring chromosome-borne <i>bla</i><sub>OXA-23</sub>, which was isolated from the bronchoalveolar lavage fluid (BALF) of a patient in a respiratory intensive care unit (RICU), we systematically analyzed antibiotic resistance genes (ARGs) and the genetic context associated with ARGs carried by CRAB strains harboring chromosome-borne <i>bla</i><sub>OXA-23</sub> worldwide. Besides <i>bla</i><sub>OXA-23</sub>, other ARGs were detected on the chromosome of the CRAB strain AB2877 belonging to ST208/1806 (Oxford MLST scheme). Several key genetic contexts associated with the ARGs were identified on the chromosome of the CRAB strain AB2877, including (1) the MDR region associated with <i>bla</i><sub>OXA-23</sub>, <i>tet(B)-tetR(B</i>), <i>aph(3'')-Ib,</i> and <i>aph(6)-Id</i> (2); the resistance island AbGRI3 harboring <i>armA</i> and <i>mph(E)-msr(E</i>) (3); the Tn<i>3</i>-like composite transposon containing <i>bla</i><sub>TEM-1D</sub> and <i>aph(3')-Ia</i>; and (4) the structure \"IS<i>Aba1-bla</i><sub>ADC-25</sub>.\" The first two genetic contexts were most common in ST195/1816, followed by ST208/1806. The last two genetic contexts were found most frequently in ST208/1806, followed by ST195/1816.IMPORTANCEThe <i>bla</i><sub>OXA-23</sub> gene can be carried by plasmid or chromosome, facilitating horizontal genetic transfer and increasing carbapenem resistance in healthcare settings. In this study, we focused on the genomic characteristics of CRAB strains harboring the chromosome-borne <i>bla</i><sub>OXA-23</sub> gene, and the important genetic contexts associated with <i>bla</i><sub>OXA-23</sub> and other ARGs were identified, and their prevalent clones worldwide were determined. Notably, although the predominant clonal CRAB lineages worldwide containing the MDR region associated with <i>bla</i><sub>OXA-23</sub>, <i>tet(B)-tetR(B</i>), <i>aph(3'')-Ib,</i> and <i>aph (6)-Id</i> was ST195/1816, followed by ST208/1806, the CRAB strain AB2877 in our study belonged to ST208/1806. Our findings contribute to the knowledge regarding the dissemination of CRAB strains and the control of nosocomial infection.</p>","PeriodicalId":18670,"journal":{"name":"Microbiology spectrum","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11370241/pdf/","citationCount":"0","resultStr":"{\"title\":\"Whole genome sequencing insight into carbapenem-resistant and multidrug-resistant <i>Acinetobacter baumannii</i> harboring chromosome-borne <i>bla</i><sub>OXA-23</sub>.\",\"authors\":\"Wei Wang, Jiahui Weng, Jie Wei, Qinghuan Zhang, Yu Zhou, Yanju He, Limei Zhang, Wenting Li, Yi Zhang, Zhiren Zhang, Xiaobin Li\",\"doi\":\"10.1128/spectrum.00501-24\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) poses a significant threat to hospitalized patients as effective therapeutic options are scarce. Based on the genomic characteristics of the CRAB strain AB2877 harboring chromosome-borne <i>bla</i><sub>OXA-23</sub>, which was isolated from the bronchoalveolar lavage fluid (BALF) of a patient in a respiratory intensive care unit (RICU), we systematically analyzed antibiotic resistance genes (ARGs) and the genetic context associated with ARGs carried by CRAB strains harboring chromosome-borne <i>bla</i><sub>OXA-23</sub> worldwide. Besides <i>bla</i><sub>OXA-23</sub>, other ARGs were detected on the chromosome of the CRAB strain AB2877 belonging to ST208/1806 (Oxford MLST scheme). Several key genetic contexts associated with the ARGs were identified on the chromosome of the CRAB strain AB2877, including (1) the MDR region associated with <i>bla</i><sub>OXA-23</sub>, <i>tet(B)-tetR(B</i>), <i>aph(3'')-Ib,</i> and <i>aph(6)-Id</i> (2); the resistance island AbGRI3 harboring <i>armA</i> and <i>mph(E)-msr(E</i>) (3); the Tn<i>3</i>-like composite transposon containing <i>bla</i><sub>TEM-1D</sub> and <i>aph(3')-Ia</i>; and (4) the structure \\\"IS<i>Aba1-bla</i><sub>ADC-25</sub>.\\\" The first two genetic contexts were most common in ST195/1816, followed by ST208/1806. The last two genetic contexts were found most frequently in ST208/1806, followed by ST195/1816.IMPORTANCEThe <i>bla</i><sub>OXA-23</sub> gene can be carried by plasmid or chromosome, facilitating horizontal genetic transfer and increasing carbapenem resistance in healthcare settings. In this study, we focused on the genomic characteristics of CRAB strains harboring the chromosome-borne <i>bla</i><sub>OXA-23</sub> gene, and the important genetic contexts associated with <i>bla</i><sub>OXA-23</sub> and other ARGs were identified, and their prevalent clones worldwide were determined. Notably, although the predominant clonal CRAB lineages worldwide containing the MDR region associated with <i>bla</i><sub>OXA-23</sub>, <i>tet(B)-tetR(B</i>), <i>aph(3'')-Ib,</i> and <i>aph (6)-Id</i> was ST195/1816, followed by ST208/1806, the CRAB strain AB2877 in our study belonged to ST208/1806. Our findings contribute to the knowledge regarding the dissemination of CRAB strains and the control of nosocomial infection.</p>\",\"PeriodicalId\":18670,\"journal\":{\"name\":\"Microbiology spectrum\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11370241/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiology spectrum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1128/spectrum.00501-24\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/5 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology spectrum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1128/spectrum.00501-24","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/5 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Whole genome sequencing insight into carbapenem-resistant and multidrug-resistant Acinetobacter baumannii harboring chromosome-borne blaOXA-23.
Carbapenem-resistant Acinetobacter baumannii (CRAB) poses a significant threat to hospitalized patients as effective therapeutic options are scarce. Based on the genomic characteristics of the CRAB strain AB2877 harboring chromosome-borne blaOXA-23, which was isolated from the bronchoalveolar lavage fluid (BALF) of a patient in a respiratory intensive care unit (RICU), we systematically analyzed antibiotic resistance genes (ARGs) and the genetic context associated with ARGs carried by CRAB strains harboring chromosome-borne blaOXA-23 worldwide. Besides blaOXA-23, other ARGs were detected on the chromosome of the CRAB strain AB2877 belonging to ST208/1806 (Oxford MLST scheme). Several key genetic contexts associated with the ARGs were identified on the chromosome of the CRAB strain AB2877, including (1) the MDR region associated with blaOXA-23, tet(B)-tetR(B), aph(3'')-Ib, and aph(6)-Id (2); the resistance island AbGRI3 harboring armA and mph(E)-msr(E) (3); the Tn3-like composite transposon containing blaTEM-1D and aph(3')-Ia; and (4) the structure "ISAba1-blaADC-25." The first two genetic contexts were most common in ST195/1816, followed by ST208/1806. The last two genetic contexts were found most frequently in ST208/1806, followed by ST195/1816.IMPORTANCEThe blaOXA-23 gene can be carried by plasmid or chromosome, facilitating horizontal genetic transfer and increasing carbapenem resistance in healthcare settings. In this study, we focused on the genomic characteristics of CRAB strains harboring the chromosome-borne blaOXA-23 gene, and the important genetic contexts associated with blaOXA-23 and other ARGs were identified, and their prevalent clones worldwide were determined. Notably, although the predominant clonal CRAB lineages worldwide containing the MDR region associated with blaOXA-23, tet(B)-tetR(B), aph(3'')-Ib, and aph (6)-Id was ST195/1816, followed by ST208/1806, the CRAB strain AB2877 in our study belonged to ST208/1806. Our findings contribute to the knowledge regarding the dissemination of CRAB strains and the control of nosocomial infection.
期刊介绍：
Microbiology Spectrum publishes commissioned review articles on topics in microbiology representing ten content areas: Archaea; Food Microbiology; Bacterial Genetics, Cell Biology, and Physiology; Clinical Microbiology; Environmental Microbiology and Ecology; Eukaryotic Microbes; Genomics, Computational, and Synthetic Microbiology; Immunology; Pathogenesis; and Virology. Reviews are interrelated, with each review linking to other related content. A large board of Microbiology Spectrum editors aids in the development of topics for potential reviews and in the identification of an editor, or editors, who shepherd each collection.
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