丙酮酸乙酯对裸鼠食管鳞癌移植瘤的影响

IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xuyang Liang, Jing Wen, Jing Xu, Zhimei Zhang, Shuxian Zhang, Lu Wang
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引用次数: 0

摘要

本研究旨在探讨丙酮酸乙酯(一种 HMGB1 抑制剂)在体外和体内对 ESCC 细胞的影响。采用 MTT 法评估 ESCC 细胞的存活率,以评估 EP 与细胞存活率之间的相关性。划痕试验用于研究 EP 与细胞迁移和侵袭之间的关系。使用肿瘤形成模型研究了 EP 对癌症裸鼠肿瘤生长和存活的影响。免疫组化染色评估了肿瘤组织中 HMGB1、TLR4 和 MyD88 的表达水平。抗 HMGB1 抑制剂 EP 可抑制 ESCC 细胞在体外和体内的增殖和转移。此外,与对照组相比,EP能改善裸鼠的活动、饮食和饮水行为,抑制肿瘤生长,并降低HMGB1、TLR4和MyD88的蛋白表达水平。EP有望调节HMGB1/TLR4-MyD88信号通路,从而抑制ESCC的增殖和转移,抑制肿瘤生长,改善生活质量,成为治疗ESCC的有效药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of ethyl pyruvate on human esophageal squamous cell carcinoma transplanted tumors in nude mice.

The objective of this study was to investigate the impact of ethyl pyruvate (EP), an HMGB1 inhibitor, on ESCC cells both in vitro and in vivo. The viability of ESCC cells was assessed using the MTT method to evaluate the correlation between EP and cell viability. A scratch test was used to investigate the relationship between EP and cell migration and invasion. The effects of EP on tumor growth and survival in cancerous nude mice were examined using a tumor formation model. Immunohistochemical staining was performed to evaluate the expression levels of HMGB1, TLR4, and MyD88 in tumor tissues. EP, an anti-HMGB1 inhibitor, inhibited ESCC cell proliferation and metastasis in vitro and in vivo. Furthermore, compared with the control treatment, EP improved the activity, diet, and drinking behaviour of nude mice; inhibited tumour growth; and led to lower protein expression levels of HMGB1, TLR4, and MyD88. EP has the potential to regulate the HMGB1/TLR4-MyD88 signaling pathway, thereby inhibiting the proliferation and metastasis of ESCC, suppressing tumor growth, improving quality of life, and serving as an effective drug for ESCC treatment.

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来源期刊
Cellular and molecular biology
Cellular and molecular biology 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
331
期刊介绍: Cellular and Molecular Biology publishes original articles, reviews, short communications, methods, meta-analysis notes, letters to editor and comments in the interdisciplinary science of Cellular and Molecular Biology linking and integrating molecular biology, biophysics, biochemistry, enzymology, physiology and biotechnology in a dynamic cell and tissue biology environment, applied to human, animals, plants tissues as well to microbial and viral cells. The journal Cellular and Molecular Biology is therefore open to intense interdisciplinary exchanges in medical, dental, veterinary, pharmacological, botanical and biological researches for the demonstration of these multiple links.
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