99mTc-HYNIC-Annexin V 检测兔肝癌模型中的细胞凋亡。

IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zuoliang Shi, Mengcheng Hu, Jianmin Bian
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引用次数: 0

摘要

为了研究 99mTc-HYNIC-Annexin V 检测体内细胞凋亡的可行性,通过 HYNIC 用 99mTc 标记 Annexin V。18 只植入 VX-2 的新西兰兔被随机分为对照组(n = 8)和紫杉醇(PAC,n = 10)组,静脉注射 2 mL/kg 生理盐水或 2.4 mg/kg PAC。分别在治疗前、治疗后24小时和48小时通过静脉注射99m锝-HYNIC-Annexin V进行SPECT肝脏肿瘤成像检测。计算肿瘤放射性计数与非肿瘤部位的比例。最后一次成像结束后,兔子被处死。取出肿瘤并分成两块,一块用于 TUNEL 免疫组化分析,另一块用于流式细胞术(FCM)。我们发现,通过 HYNIC 用 99mTc 标记的 Annexin V 的阳性率超过 95%,放射化学纯度在 95% 以上。SPECT 显示,两组患者在治疗前均无明显肿瘤影像。对照组无明显肿瘤成像,而 PAC 组在治疗后 24 h 和 48 h 出现明显蓄积。化疗后 24 小时和 48 小时,PAC 组的肿瘤/非肿瘤(T/NT)与治疗前的对照组和 PAC 组相比有显著差异。PAC 组化疗后 24 h 和 48 h 的肿瘤/非肿瘤(T/NT)与对照组和治疗前的 PAC 组无明显差异。免疫组化检测到的 TUNEL 阳性细胞和 FCM 检测到的凋亡率,PAC 组与对照组有明显差异。T/NT与肿瘤的TUNEL阳性细胞和凋亡率明显相关。PAC 可诱导兔 VX-2 肝癌细胞凋亡。紫杉醇化疗后 24-48 h 是检测细胞凋亡的窗口期。体内凋亡细胞可通过 99mTc-HYNIC-Annexin V SPECT 检测到。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
99mTc-HYNIC-Annexin V detection of apoptosis in the rabbit model of liver cancer.

To investigate the feasibility of detection of apoptosis in vivo by 99mTc-HYNIC-Annexin V, Annexin V was labeled with 99mTc through HYNIC. 18 New Zealand rabbits implanted VX-2 were randomly divided into control (n = 8) and paclitaxel (PAC, n = 10) groups, given 2 mL/kg of normal saline or 2.4 mg/kg of PAC intravenously. The liver tumor imaging was detected by SPECT through intravenous injection of 99mTc-HYNIC-Annexin V before treatment, 24 hours and 48 hours after treatment respectively. Tumor radioactive count proportion to non-tumor sites was calculated. When the last imaging was finished, the rabbits were sacrificed. The tumor was taken out and divided into two pieces, one for TUNEL immunohistochemical analysis and the other for flow cytometry (FCM). We found that the rate of Annexin V labeled with 99mTc through HYNIC was more than 95%, and radiochemical purity was above 95%. The SPECT showed that two groups had no significant tumor imaging before the treatment. There is no significant tumor imaging in control group, while the PAC group 24 h and 48 h after treatment showed significant accumulation. The Tumor/non-Tumor (T/NT) in PAC group at 24 h and 48 h after chemotherapy was significantly different from that in the control group and PAC group prior to treatment. There was no significant difference between 24 h and 48 h in PAC group. The TUNEL-positive cells detected by immunohistochemistry and apoptotic rate detected by FCM in PAC group were significant different from those in control group. The T/NT was significantly correlated to TUNEL-positive cells and apoptotic rate of the tumor. PAC can induce apoptosis of rabbit VX-2 liver cancer cells. 24-48 h after paclitaxel chemotherapy is a window time for apoptosis detection. Apoptotic cells in vivo can be detected by SPECT through 99mTc-HYNIC-Annexin V.

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来源期刊
Cellular and molecular biology
Cellular and molecular biology 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
331
期刊介绍: Cellular and Molecular Biology publishes original articles, reviews, short communications, methods, meta-analysis notes, letters to editor and comments in the interdisciplinary science of Cellular and Molecular Biology linking and integrating molecular biology, biophysics, biochemistry, enzymology, physiology and biotechnology in a dynamic cell and tissue biology environment, applied to human, animals, plants tissues as well to microbial and viral cells. The journal Cellular and Molecular Biology is therefore open to intense interdisciplinary exchanges in medical, dental, veterinary, pharmacological, botanical and biological researches for the demonstration of these multiple links.
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