从鼻腔带菌者和切口感染门诊患者中分离出的对甲氧西林敏感的金黄色葡萄球菌中部分免疫逃避基因的流行率和克隆多样性

Gabriela Jura, Helena Masiuk, Agata Pruss, Mateusz Kurzawski, Monika Sienkiewicz, Iwona Wojciechowska-Koszko, Paweł Kwiatkowski
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引用次数: 0

摘要

金黄色葡萄球菌是最常见的人类病原体之一,在医院和社区环境中都会造成感染。金黄色葡萄球菌之所以具有毒性,是因为它能够通过产生免疫逃避(IE)蛋白来逃避免疫系统。本研究旨在检测从无亲属关系的门诊患者中分离出的 86 株甲氧西林易感金黄色葡萄球菌(MSSA)中属于免疫逃避群(IEC)的选定 IE 基因(spin、sbi、sea、sak、chp、scin、sep、ecb)和 IEC 类型的频率。为了确定所分析菌株的多样性,还对其系统发育相关性进行了测定。使用聚合酶链反应检测法检查了所有菌株是否存在 IE 基因。为了分析金黄色葡萄球菌的克隆亲缘关系,进行了脉冲场凝胶电泳(PFGE)。所有被分析的菌株都含有 scn 基因,其次是 sbi(95.4%)、ecb(91.7%)、spin(89.5%)、sak(83.7%)、chp(67.4%)、sep(67.4%)和 sea(5.8%)。73株(84.9%)金黄色葡萄球菌被分为 IEC 型,其中最常见的是 IEC F 型。未检测到 IEC A 型。PFGE 结果显示,克隆相关性与 IE 基因/IEC 类型的存在之间没有关联。总之,在分析的菌株中,决定入侵的基因种类繁多,这可能证明这些菌株非常先进,能够躲避宿主的免疫反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prevalence of Selected Immune Evasion Genes and Clonal Diversity in Methicillin-Susceptible Staphylococcus aureus Isolated from Nasal Carriers and Outpatients with Cut Wound Infections
Staphylococcus aureus, being one of the most common human pathogens, is responsible for infections in both hospital and community settings. Its virulence is attributed to its ability to evade the immune system by producing immune evasion (IE) proteins. The aim of this study was to detect the frequency of selected IE genes (spin, sbi, sea, sak, chp, scin, sep, ecb), belonging to the immune evasion cluster (IEC), and IEC types in 86 methicillin-susceptible S. aureus (MSSA) strains isolated from unrelated outpatients. In order to determine the diversity of analyzed strains, the phylogenetic relatedness was also determined. All strains were examined for the presence of IE genes using polymerase chain reaction assay. To analyze the clonal relatedness of S. aureus, pulsed-field gel electrophoresis (PFGE) was performed. All analyzed strains harbored the scn gene, followed by sbi (95.4%), ecb (91.7%), spin (89.5%), sak (83.7%), chp (67.4%), sep (67.4%) and sea (5.8%). Seventy-three (84.9%) S. aureus strains were classified into IEC types, of which, IEC type F was most commonly observed. IEC type A was not detected. PFGE results showed no association between clonal relatedness and the presence of IE genes/IEC types. In conclusion, the abundant and so diverse repertoire of genes determining invasion in analyzed strains may prove the fact that these strains are highly advanced and adapted to evade the host immune response.
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