地塞米松疗法会影响大鼠腹主动脉模型损伤后的内膜增生吗?

Çağla Canbay Sarılar, Omer Ali Sayın, Mert Sarılar, Nilgun Bozbuga, İbrahim Demir, Vakur Olgac, İbrahim Ufuk Alpagut
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引用次数: 0

摘要

背景:内膜增生是动脉对损伤(包括侵入性血管干预)的正常适应性反应。内膜增生的发展限制了搭桥移植物的长期成功。在实验模型中已成功使用了多种药理制剂来降低内膜增生的程度。在我们的研究中,我们探讨了地塞米松在减少大鼠腹主动脉部分横断和初级修复后内膜增生方面的功效:本研究随机选取 20 只 Wistar Albino 大鼠,将其分为四组,与对照组相比,比较低剂量和高剂量地塞米松对内膜和介质厚度的影响。A组(n=5)为对照组,只进行皮肤切口和开腹手术。B组(n=5)进行正中开腹手术,部分横断腹主动脉,并用8.0prolene缝合线修复。C 组(n=5)和 D 组(n=5)分别注射 0.1 mg/kg 和 0.2 mg/kg 地塞米松。两周后,所有大鼠安乐死,切除修复后的腹主动脉并进行组织病理学检查。在拍摄数码照片后,使用 "Olympus AnalySIS 5 "程序(日本奥林巴斯公司)测量内膜和中膜厚度:根据测量结果,我们发现在横断和修复腹主动脉后,低剂量地塞米松组和非地塞米松组之间的内膜/中膜比率没有显著差异。高剂量地塞米松组的内膜/中膜比率明显低于非地塞米松组和低剂量地塞米松组:结论:血管介入治疗后,地塞米松治疗可减少内膜增生,并通过血管重塑增加通畅率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Does dexamethasone therapy affect intimal hyperplasia after injury in rat abdominal aorta models?

Background: Intimal hyperplasia is a normal adaptive feature of arteries in response to injuries, which include invasive vascular interventions. Its development limits the long-term success of bypass grafts. Various pharmacological agents have been successfully employed in experimental models to reduce the degree of intimal hyperplasia. In our study, we investigated the efficacy of dexamethasone in reducing intimal hyperplasia in rat abdominal aortas after partial transection and primary repair.

Methods: In this study, 20 Wistar Albino rats were randomly selected and divided into four groups to compare the effects of low- and high-dose dexamethasone on intima and media thickness compared to the control. Group A (n=5) was the control group, where only skin incision and laparotomy were performed. For Group B (n=5), a median laparotomy was performed, the abdominal aorta was partially transected, and repaired with an 8.0 prolene suture. Doses of 0.1 mg/kg and 0.2 mg/kg dexamethasone were administered in Group C (n=5) and Group D (n=5), respectively. After two weeks, all rats were euthanized, and the repaired abdominal aortas were excised and examined histopathologically. Intima and media thicknesses were measured using the 'Olympus AnalySIS 5' program (Olympus Corporation, Japan) after digital photos were taken.

Results: Based on the measurements, we demonstrated that after transection and repair of the abdominal aorta, the intima/media ratio was not significantly different between the low-dose dexamethasone and non-dexamethasone groups. The intima/media ratio was significantly lower in the high-dose dexamethasone group than in the non-dexamethasone and low-dose dexamethasone groups.

Conclusion: After vascular interventions, dexamethasone treatment may reduce intimal hyperplasia and increase patency by providing vascular remodeling.

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