利用纳米电动漂移预富集技术对 DNA 混合物进行分类

IF 5.5 3区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Seongho Baek, Jihee Park, Hobin Sung, Jung Ae Lee, Man Jin Kim, Moon-Woo Seong, Sung Jae Kim
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引用次数: 0

摘要

纳米流体现象,特别是离子浓度极化(ICP),在过去的一个世纪中被积极地用于推进包括化学分析和生物医学诊断在内的各个研究领域。虽然 ICP 可作为生物/化学分析中有效的预浓缩技术,但很少有研究探讨预浓缩塞的形状,尤其是分析物在预浓缩塞中的垂直分布。以前我们曾报道过对分布的理论分析,从而将塞子的类型分为哑铃形和塞子形。在本研究中,我们通过研究不同类型 DNA 在不同电气条件下的预浓缩动态,进一步研究了微/纳流控设备中真实 DNA 的分类。通过引入荧光回旋半径(RGF)的概念,我们的研究成功区分了短 DNA、多短 DNA 和等效 DNA 的不同预浓缩特征。我们提供了一个定量框架,以合理的精度分析和区分预浓缩形状。这些发现不仅加深了我们对 DNA 预浓缩动力学的理解,还为基因诊断提供了启示。作为一种更简单、更易用的预检测工具,我们的研究可用于高效的基因检测,尤其是诊断以 DNA 长度变化为特征的疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Classification of DNA Mixtures by Nanoelectrokinetic Driftless Preconcentration

Classification of DNA Mixtures by Nanoelectrokinetic Driftless Preconcentration

Nanofluidic phenomena, particularly Ion Concentration Polarization (ICP), have been actively utilized for advancing various research fields, including chemical analysis and biomedical diagnostics, over the past century. While ICP can be used as effective preconcentration techniques in bio-/chemical analysis, there are few studies to investigate the shape of preconcentration plug, especially perpendicular distribution of analyte in the preconcentration plug. Previously we have reported the theoretical analysis of the distribution so that the types of plug were categorized as dumbbell or plug shape. In this study, we further investigated the classification of real DNAs within micro-/nanofluidic devices by examining the preconcentration dynamics of different DNA types under diverse electrical conditions. Our investigation successfully distinguished distinct preconcentration profiles for Short DNA, Multi-short DNAs, and Equitable DNA with introducing the concept of the Radius of Gyration for Fluorescence (RGF). We provided a quantitative framework to analyze and differentiate preconcentration shapes with reasonable precision. These findings not only deepen our understanding of DNA preconcentration dynamics but also provide implications for genetic diagnostics. As a simpler and more accessible pre-test tool, our research could be utilized as the efficient genetic testing, particularly in diagnosing disorders characterized by variations in DNA length.

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来源期刊
BioChip Journal
BioChip Journal 生物-生化研究方法
CiteScore
7.70
自引率
16.30%
发文量
47
审稿时长
6-12 weeks
期刊介绍: BioChip Journal publishes original research and reviews in all areas of the biochip technology in the following disciplines, including protein chip, DNA chip, cell chip, lab-on-a-chip, bio-MEMS, biosensor, micro/nano mechanics, microfluidics, high-throughput screening technology, medical science, genomics, proteomics, bioinformatics, medical diagnostics, environmental monitoring and micro/nanotechnology. The Journal is committed to rapid peer review to ensure the publication of highest quality original research and timely news and review articles.
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