Mannava Laharika Choudary, Medidi Srinivas, Bula Udaya Kumari
{"title":"采用 RP-HPLC/UV 法开发和验证胶囊剂型中依替福辛的估算方法","authors":"Mannava Laharika Choudary, Medidi Srinivas, Bula Udaya Kumari","doi":"10.22270/jddt.v14i7.6703","DOIUrl":null,"url":null,"abstract":"A RP-HPLC/UV method was developed and validated to quantify the etifoxine in capsule dosage form. The chromatographic separation was carried out using a Hypersil ODS C18 column (250×4.6 mm, 5 μm) with a mobile phase consisting of an ammonium acetate buffer: acetonitrile in a volumetric ratio of 40:60%. The UV wavelength chosen for detection was at 255 nm. The flow rate was set at 1 ml/min. The retention time for etifoxine was determined to be 2.074 min. Linearity was detected within the concentration range of 7.5-45 μg/mL for etifoxine. The approach has been confirmed to be linear, accurate, precise, robust, and has established limits of detection and quantitation. The established procedure was uncomplicated, cost-effective, and suitable for the routine analysis of etifoxine in capsule dosage form.","PeriodicalId":15622,"journal":{"name":"Journal of Drug Delivery and Therapeutics","volume":" 7","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Method Development and Validation for Estimation of Etifoxine in Capsule Dosage Form by Using RP-HPLC/UV\",\"authors\":\"Mannava Laharika Choudary, Medidi Srinivas, Bula Udaya Kumari\",\"doi\":\"10.22270/jddt.v14i7.6703\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A RP-HPLC/UV method was developed and validated to quantify the etifoxine in capsule dosage form. The chromatographic separation was carried out using a Hypersil ODS C18 column (250×4.6 mm, 5 μm) with a mobile phase consisting of an ammonium acetate buffer: acetonitrile in a volumetric ratio of 40:60%. The UV wavelength chosen for detection was at 255 nm. The flow rate was set at 1 ml/min. The retention time for etifoxine was determined to be 2.074 min. Linearity was detected within the concentration range of 7.5-45 μg/mL for etifoxine. The approach has been confirmed to be linear, accurate, precise, robust, and has established limits of detection and quantitation. The established procedure was uncomplicated, cost-effective, and suitable for the routine analysis of etifoxine in capsule dosage form.\",\"PeriodicalId\":15622,\"journal\":{\"name\":\"Journal of Drug Delivery and Therapeutics\",\"volume\":\" 7\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Drug Delivery and Therapeutics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22270/jddt.v14i7.6703\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Drug Delivery and Therapeutics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22270/jddt.v14i7.6703","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Method Development and Validation for Estimation of Etifoxine in Capsule Dosage Form by Using RP-HPLC/UV
A RP-HPLC/UV method was developed and validated to quantify the etifoxine in capsule dosage form. The chromatographic separation was carried out using a Hypersil ODS C18 column (250×4.6 mm, 5 μm) with a mobile phase consisting of an ammonium acetate buffer: acetonitrile in a volumetric ratio of 40:60%. The UV wavelength chosen for detection was at 255 nm. The flow rate was set at 1 ml/min. The retention time for etifoxine was determined to be 2.074 min. Linearity was detected within the concentration range of 7.5-45 μg/mL for etifoxine. The approach has been confirmed to be linear, accurate, precise, robust, and has established limits of detection and quantitation. The established procedure was uncomplicated, cost-effective, and suitable for the routine analysis of etifoxine in capsule dosage form.
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