尼日利亚贝宁大学食品摊贩手和鼻拭子细菌分离概况

A.S. Aziegbemhin, A. Dunkwu-Okafor
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引用次数: 0

摘要

本研究旨在调查从埃多州贝宁市贝宁大学食品摊贩的手和鼻拭子中分离出的细菌的细菌学特征。研究人员从尼日利亚贝宁市贝宁大学美食广场的十(10)个不同食品摊贩处随机采集了共 20 份不同的样本。拭子样本用条纹平板接种,在 37oC 温度下培养 24 小时。24 小时后对样本进行亚培养,并根据培养特征、形态和生化测试进行鉴定。从鼻拭子样本中分离出的细菌包括表皮葡萄球菌、金黄色葡萄球菌和蜡样芽孢杆菌,从手拭子样本中分离出的细菌包括表皮葡萄球菌和金黄色葡萄球菌。从手拭子样本中分离出的细菌出现频率分别为表皮葡萄球菌 7(70%)、金黄色葡萄球菌 3(30%),而从鼻拭子样本中分离出的细菌出现频率分别为金黄色葡萄球菌 5(50%)、表皮葡萄球菌 2(20%)和蜡样芽孢杆菌 3(30%)。从鼻拭子中分离出的金黄色葡萄球菌对抗生素的敏感性和耐药性模式显示,所有分离物中有 10 个(100%)分别对培氟沙星、环丙沙星、链霉素和共三唑(septrin)敏感,而 0 个(0%)对头孢呋辛耐药,而从手拭子中分离出的表皮葡萄球菌有 5 个(71.而从手拭子中分离出的表皮葡萄球菌则有 5 株(71.4%)对庆大霉素敏感,3 株(42.9%)对氨苄西林敏感,2 株(28.6%)对罗红霉素敏感,5 株(57.1%)对红霉素敏感。因此,建议食品摊贩应严格遵守符合法规标准的良好卫生习惯和健康规范。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bacteriological profile of bacterial isolates from the hands and nasal swabs of food vendors in University of Benin, Nigeria
This research was undertaken to investigate the bacteriological profile of bacterial isolates from the hands and nasal swabs of food vendors at the University of Benin, Benin City, Edo State. A total of 20 different samples were randomly collected from ten (10) different food vendors at the University of Benin Food Court, Benin City, Nigeria. Swab samples were inoculated using streak plate and incubated at 24 hours at 37oC. The samples were sub-cultured after 24 hours and identified based on cultural characteristics, morphology and biochemical tests. The bacteria isolated include Staphylococcus epidermidis, Staphylococcus aureus, and Bacillus cereus from nasal swab samples while Staphylococcus epidermidis and Staphylococcus aureus were isolated from hand swab samples. The frequency of occurrence of the bacteria recovered from hand was Staphylococcus epidermidis 7(70%), Staphylococcus aureus 3(30%) while Staphylococcus aureus 5(50%), Staphylococcus epidermidis 2(20%) and Bacillus cereus 3(30%) were recovered from nasal swab respectively. The antibiotic susceptibility and resistance pattern exhibited by the recovered isolates from the nasal swab showed that 10(100%) of all the isolates were susceptible to Pefloxacin, Ciprofloxacin, Streptomycin and Co-trimoxazole(septrin) respectively while 0(0%) of the recovered isolates were resistant to Cefuroxime respectively whereas the recovered Staphylococcus epidermidis from the hand swab had 5 (71.4%) which were susceptible to Gentamycin, 3(42.9%) which were susceptible to Ampiclox, 2(28.6%) which were susceptible to Rocephin, and 5(57.1%) were susceptible to Erythromycin. Consequently, it is suggested that the food vendors should adhere strictly to good hygiene practices and health practices in line with regulatory standards.
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