含鱼油的食用薄膜,其活性薄膜中加入了番石榴叶提取物:双层食用薄膜的制备和表征

Aji Sukoco, Yukihiro Yamamoto, Hiroyuki Harada, Atsushi Hashimoto, Tomoyuki Yoshino
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The upper/active layer was prepared by mixing MG complex, glycerol, and GLE (1, 3, and 5% w/v of the complex). Physical, mechanical, microstructural, thermal, microbiological, and oxidative measurements were also performed. Results The total phenolic and flavonoid contents in GLE were 15.81 mg GAE/g extract and 6.99 mg QE/g extract, respectively. The IC50 of the DPPH radical scavenging activity of GLE was 26.86 ppm with antibacterial activity against Bacillus subtilis and Escherichia coli of 9.83 and 12.55 mm. The total plate counts of films double-layered with a film containing GLE were retained below 3 log CFU/g during 28-day storage. The peroxide values of these films were dimmed for no more than 9.08 meq/kg sample on day 28 of storage. 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引用次数: 0

摘要

背景 玉米蛋白和阿拉伯树胶的使用量不断增加,试图在蛋白质-多糖复合物的基础上配制壁材。这种混合物为亲水性(番石榴叶提取物,GLE)或亲油性(鱼油,FO)生物活性化合物提供了一个多功能庇护所,使其免受有害环境因素的影响,还可用作可食用的成膜聚合物。本研究的目的是描述含有 FO 的可食用薄膜与含有 GLE 的薄膜双层的特性。方法 将改性玉米蛋白和阿拉伯树胶溶液(MG 复合物)按 1:1.5 的比例(v/v)混合,调节 pH 值至 5,加入甘油(复合物的 20%)和 FO(复合物的 5%),最后调节 pH 值至 5。上层/活性层是通过混合 MG 复合物、甘油和 GLE(复合物的 1%、3% 和 5% w/v)制备的。此外,还进行了物理、机械、微结构、热、微生物和氧化测量。结果 GLE 中的总酚和类黄酮含量分别为 15.81 毫克 GAE/克提取物和 6.99 毫克 QE/克提取物。GLE 的 DPPH 自由基清除活性 IC50 为 26.86 ppm,对枯草杆菌和大肠杆菌的抗菌活性分别为 9.83 和 12.55 mm。含有 GLE 的双层薄膜的菌落总数在 28 天的储存期间保持在 3 log CFU/g 以下。在储存的第 28 天,这些薄膜的过氧化值降低到不超过 9.08 meq/kg 样品。厚度(872.00-971.67 微米)、水蒸气透过率(12.99-17.04 克/平方米/天)、拉伸强度(1.56-2.02 千帕)、断裂伸长率(61.53-75.41%)、玻璃化转变(52.74-57.50°C)、熔点(131.59-142.35°C)、对枯草杆菌的抑制率(33.67-40.58 毫米)和对大肠杆菌的抑制率(2.05-9.04 毫米)。结论 GLE 可以成功地加入到双层薄膜的活性层中,以改善其特性,同时显著减缓微生物污染和氧化速度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fish oil-containing edible films with active film incorporated with extract of Psidium guajava leaves: preparation and characterization of double-layered edible film
Background The utilization of zein and gum arabic has grown in an attempt to formulate wall materials based on protein–polysaccharide complexes. This mixture provides a versatile shelter for hydrophilic (guava leaf extract, GLE) or lipophilic (fish oil, FO) bioactive compounds from unwanted environmental factors, and it can be used as an edible film-forming polymer. This study was undertaken to characterize FO-containing edible films that were double-layered with a film containing GLE. Methods Modified zein and gum arabic solutions (MG complex) were mixed at a ratio of 1:1.5 (v/v), adjusted to pH 5, added with glycerol (20% of the complex) and FO (5% of the complex), and finally adjusted to pH 5. This was prepared as the bottom/lower layer. The upper/active layer was prepared by mixing MG complex, glycerol, and GLE (1, 3, and 5% w/v of the complex). Physical, mechanical, microstructural, thermal, microbiological, and oxidative measurements were also performed. Results The total phenolic and flavonoid contents in GLE were 15.81 mg GAE/g extract and 6.99 mg QE/g extract, respectively. The IC50 of the DPPH radical scavenging activity of GLE was 26.86 ppm with antibacterial activity against Bacillus subtilis and Escherichia coli of 9.83 and 12.55 mm. The total plate counts of films double-layered with a film containing GLE were retained below 3 log CFU/g during 28-day storage. The peroxide values of these films were dimmed for no more than 9.08 meq/kg sample on day 28 of storage. Thickness (872.00-971.67 μm), water vapor transmission rate (12.99-17.04 g/m2/day), tensile strength (1.56-2.02 kPa), elongation at break (61.53-75.41%), glass transition (52.74-57.50°C), melting peak (131.59-142.35°C), inhibition against B. subtilis (33.67-40.58 mm), and inhibition against E. coli (2.05-9.04 mm) were obtained by double-layer films. Conclusions GLE can be successfully incorporated into the active layer of a double-layer film to improve its characteristics while significantly slowing down the microbial contamination and oxidation rate.
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