{"title":"沙漠椰枣和辣木籽精油在实验室条件下对 Helicoverpa armigera Hub (Lepidoptera: Pyralidae) 幼虫的毒性","authors":"G. Abdullahi, H. Sule, H. A. Yerima","doi":"10.36108/nje/4202/04.0210","DOIUrl":null,"url":null,"abstract":"Production of tomato, Solanum lycopersicum worldwide is being threatened by Helicoverpa armigera Hub., and farmers rely heavily on the use of costly hazardous synthetic insecticides for its management. The prime concern in increasing production of tomato is the obstacles posed by H. armigera and lack of sufficient understanding of available environmentally friendly control materials which are essential for the development of reliable integrated pest management strategies. The present study was conducted with the objectives of determining the toxicities of desert dates and moringa seed essential oils (EO) on the larvae of H. armigera. The two (2) EOs were obtained by distillation and evaporation techniques. Toxicity studies were conducted at the Entomology laboratory of the Department of Crop Protection, Bayero University Kano. Fruits and leaves of tomato plant were sprayed with different concentrations of 2%, 4% and 6% of the essential oil extracted from the plant and acetone was used as control, cypermethrin (synthetic insecticide) was used as a standard check. The treated plants parts were allowed to dry for 15 to 20 minutes under laboratory condition. Ten 3rd instar larvae of H. armigera were introduced into the container covered with muslin cloth and allowed to feed. Observation on larval mortality was made at 24, 48 and 72h post introduction. EOs composition was analysed using GC-MS for qualitative and quantitative determination of the biomolecules present. Results of the toxicity of the essential oils show both oils caused mortality of the larvae. The GC-MS analysis shows 1-Methylpentyl cyclopropane (Monoterpene) as the dominant compound in both essential oils. Two percent (2%) EO of balanites is recommended for application on both the fruits and leaves of the tomato plant to control H. amigera","PeriodicalId":183712,"journal":{"name":"Nigerian Journal of Entomology","volume":"65 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Toxicities of Desert Dates and Moringa Seeds Essential Oils on Helicoverpa armigera Hub (Lepidoptera: Pyralidae) Larvae under Laboratory conditions\",\"authors\":\"G. Abdullahi, H. Sule, H. A. Yerima\",\"doi\":\"10.36108/nje/4202/04.0210\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Production of tomato, Solanum lycopersicum worldwide is being threatened by Helicoverpa armigera Hub., and farmers rely heavily on the use of costly hazardous synthetic insecticides for its management. The prime concern in increasing production of tomato is the obstacles posed by H. armigera and lack of sufficient understanding of available environmentally friendly control materials which are essential for the development of reliable integrated pest management strategies. The present study was conducted with the objectives of determining the toxicities of desert dates and moringa seed essential oils (EO) on the larvae of H. armigera. The two (2) EOs were obtained by distillation and evaporation techniques. Toxicity studies were conducted at the Entomology laboratory of the Department of Crop Protection, Bayero University Kano. Fruits and leaves of tomato plant were sprayed with different concentrations of 2%, 4% and 6% of the essential oil extracted from the plant and acetone was used as control, cypermethrin (synthetic insecticide) was used as a standard check. The treated plants parts were allowed to dry for 15 to 20 minutes under laboratory condition. Ten 3rd instar larvae of H. armigera were introduced into the container covered with muslin cloth and allowed to feed. Observation on larval mortality was made at 24, 48 and 72h post introduction. EOs composition was analysed using GC-MS for qualitative and quantitative determination of the biomolecules present. Results of the toxicity of the essential oils show both oils caused mortality of the larvae. The GC-MS analysis shows 1-Methylpentyl cyclopropane (Monoterpene) as the dominant compound in both essential oils. 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引用次数: 0
摘要
全世界番茄(Solanum lycopersicum)的生产正受到Helicoverpa armigera Hub.的威胁,农民严重依赖使用昂贵的有害合成杀虫剂进行防治。番茄增产的首要问题是 H. armigera 带来的障碍,以及对可用的环境友好型防治材料缺乏足够了解,而这些材料对于制定可靠的虫害综合防治战略至关重要。本研究旨在确定沙漠椰枣和辣木籽精油(EO)对胡蜂幼虫的毒性。这两种精油是通过蒸馏和蒸发技术获得的。毒性研究在卡诺巴耶罗大学作物保护系昆虫学实验室进行。番茄的果实和叶子分别喷洒了 2%、4% 和 6% 的植物精油,丙酮作为对照,氯氰菊酯(合成杀虫剂)作为标准对照。在实验室条件下,将处理过的植物部分晾干 15 至 20 分钟。将十只 H. armigera 的三龄幼虫放入铺有薄纱的容器中,让其觅食。在引入后 24、48 和 72 小时观察幼虫死亡率。使用气相色谱-质谱(GC-MS)分析精油成分,对其中的生物大分子进行定性和定量测定。精油的毒性结果表明,两种精油都会导致幼虫死亡。气相色谱-质谱分析表明,1-甲基戊基环丙烷(单萜)是两种精油中的主要化合物。建议在番茄的果实和叶片上施用百分之二(2%)的巴拉那龙精油来控制 H. amigera。
Toxicities of Desert Dates and Moringa Seeds Essential Oils on Helicoverpa armigera Hub (Lepidoptera: Pyralidae) Larvae under Laboratory conditions
Production of tomato, Solanum lycopersicum worldwide is being threatened by Helicoverpa armigera Hub., and farmers rely heavily on the use of costly hazardous synthetic insecticides for its management. The prime concern in increasing production of tomato is the obstacles posed by H. armigera and lack of sufficient understanding of available environmentally friendly control materials which are essential for the development of reliable integrated pest management strategies. The present study was conducted with the objectives of determining the toxicities of desert dates and moringa seed essential oils (EO) on the larvae of H. armigera. The two (2) EOs were obtained by distillation and evaporation techniques. Toxicity studies were conducted at the Entomology laboratory of the Department of Crop Protection, Bayero University Kano. Fruits and leaves of tomato plant were sprayed with different concentrations of 2%, 4% and 6% of the essential oil extracted from the plant and acetone was used as control, cypermethrin (synthetic insecticide) was used as a standard check. The treated plants parts were allowed to dry for 15 to 20 minutes under laboratory condition. Ten 3rd instar larvae of H. armigera were introduced into the container covered with muslin cloth and allowed to feed. Observation on larval mortality was made at 24, 48 and 72h post introduction. EOs composition was analysed using GC-MS for qualitative and quantitative determination of the biomolecules present. Results of the toxicity of the essential oils show both oils caused mortality of the larvae. The GC-MS analysis shows 1-Methylpentyl cyclopropane (Monoterpene) as the dominant compound in both essential oils. Two percent (2%) EO of balanites is recommended for application on both the fruits and leaves of the tomato plant to control H. amigera