{"title":"施用腐植酸对玉米种植下细菌多样性的影响","authors":"Mona Ayu Santi, Saowalak Somboon, Sukanya Thip-Amat, Tanabhat-Sakorn Sukitprapanon, Phrueksa Lawongsa","doi":"10.1002/agg2.20547","DOIUrl":null,"url":null,"abstract":"<p>The fundamental ecosystem processes in soil are regulated by microbial communities, and community diversity is implicated by soil environmental conditions. Humic acid (HA) improves soil quality and fertility, stimulating the microbial environment, but the detailed effects remain poorly understood. We investigated the effects of HA rates on soil bacterial diversity, particularly on species richness and community composition in the rhizosphere of corn (<i>Zea mays</i>). Inorganic fertilizer (T2), HA 0.5% (T3), HA 1.0% (T4), HA 1.5% (T5), and HA 2.0% (T6) were applied in soil. Initial soil (O1) and control after harvesting (T1) were included. A total of 3601 operational taxonomic units were captured from the overall sample, and analysis of 16S ribosomal ribonucleic acid amplicon sequencing data indicated that HA did not notably impact species richness. Intriguingly, HA induced changes in bacterial community composition, along with the relative prevalence of specific taxa. Certain associations between soil chemical properties and abundance distribution have been uncovered. Notably, exchangeable Mg<sup>2+</sup>, Ca<sup>2+</sup>, and available phosphorus were strongly related to the relative abundance of bacterial phyla. Furthermore, HA potentially shaped the specific bacterial taxa, as the application of HA at different rates had distinct effects on the member of bacterial abundance of each taxon. These findings enhance our understanding of communities potentially being increased or shifted by HA rate addition in short-term corn cultivation.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.20547","citationCount":"0","resultStr":"{\"title\":\"Effects of humic acid application on bacterial diversity under maize cultivation\",\"authors\":\"Mona Ayu Santi, Saowalak Somboon, Sukanya Thip-Amat, Tanabhat-Sakorn Sukitprapanon, Phrueksa Lawongsa\",\"doi\":\"10.1002/agg2.20547\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The fundamental ecosystem processes in soil are regulated by microbial communities, and community diversity is implicated by soil environmental conditions. Humic acid (HA) improves soil quality and fertility, stimulating the microbial environment, but the detailed effects remain poorly understood. We investigated the effects of HA rates on soil bacterial diversity, particularly on species richness and community composition in the rhizosphere of corn (<i>Zea mays</i>). Inorganic fertilizer (T2), HA 0.5% (T3), HA 1.0% (T4), HA 1.5% (T5), and HA 2.0% (T6) were applied in soil. Initial soil (O1) and control after harvesting (T1) were included. A total of 3601 operational taxonomic units were captured from the overall sample, and analysis of 16S ribosomal ribonucleic acid amplicon sequencing data indicated that HA did not notably impact species richness. Intriguingly, HA induced changes in bacterial community composition, along with the relative prevalence of specific taxa. Certain associations between soil chemical properties and abundance distribution have been uncovered. Notably, exchangeable Mg<sup>2+</sup>, Ca<sup>2+</sup>, and available phosphorus were strongly related to the relative abundance of bacterial phyla. Furthermore, HA potentially shaped the specific bacterial taxa, as the application of HA at different rates had distinct effects on the member of bacterial abundance of each taxon. These findings enhance our understanding of communities potentially being increased or shifted by HA rate addition in short-term corn cultivation.</p>\",\"PeriodicalId\":7567,\"journal\":{\"name\":\"Agrosystems, Geosciences & Environment\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-07-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.20547\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agrosystems, Geosciences & Environment\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/agg2.20547\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agrosystems, Geosciences & Environment","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/agg2.20547","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
摘要
土壤中的基本生态系统过程由微生物群落调节,群落多样性与土壤环境条件有关。腐植酸(HA)能改善土壤质量和肥力,刺激微生物环境,但其具体影响仍鲜为人知。我们研究了腐植酸浓度对土壤细菌多样性的影响,尤其是对玉米(玉米)根瘤菌群中物种丰富度和群落组成的影响。在土壤中施用无机肥料(T2)、HA 0.5%(T3)、HA 1.0%(T4)、HA 1.5%(T5)和 HA 2.0%(T6)。其中包括初始土壤(O1)和收获后的对照(T1)。从总体样本中总共捕获了 3601 个可操作的分类单元,对 16S 核糖体核糖核酸扩增片段测序数据的分析表明,HA 并未对物种丰富度产生显著影响。耐人寻味的是,HA 引发了细菌群落组成的变化,以及特定类群的相对流行率。土壤化学特性与丰度分布之间的某些联系已经被发现。值得注意的是,可交换的 Mg2+、Ca2+ 和可用磷与细菌类群的相对丰度密切相关。此外,HA 可能会影响特定的细菌类群,因为以不同的速率施用 HA 会对每个类群的细菌丰度产生不同的影响。这些发现加深了我们对短期玉米种植中添加 HA 可能会增加或改变群落的了解。
Effects of humic acid application on bacterial diversity under maize cultivation
The fundamental ecosystem processes in soil are regulated by microbial communities, and community diversity is implicated by soil environmental conditions. Humic acid (HA) improves soil quality and fertility, stimulating the microbial environment, but the detailed effects remain poorly understood. We investigated the effects of HA rates on soil bacterial diversity, particularly on species richness and community composition in the rhizosphere of corn (Zea mays). Inorganic fertilizer (T2), HA 0.5% (T3), HA 1.0% (T4), HA 1.5% (T5), and HA 2.0% (T6) were applied in soil. Initial soil (O1) and control after harvesting (T1) were included. A total of 3601 operational taxonomic units were captured from the overall sample, and analysis of 16S ribosomal ribonucleic acid amplicon sequencing data indicated that HA did not notably impact species richness. Intriguingly, HA induced changes in bacterial community composition, along with the relative prevalence of specific taxa. Certain associations between soil chemical properties and abundance distribution have been uncovered. Notably, exchangeable Mg2+, Ca2+, and available phosphorus were strongly related to the relative abundance of bacterial phyla. Furthermore, HA potentially shaped the specific bacterial taxa, as the application of HA at different rates had distinct effects on the member of bacterial abundance of each taxon. These findings enhance our understanding of communities potentially being increased or shifted by HA rate addition in short-term corn cultivation.