利用废弃有机物转化生产生物燃料的微生物诱导生物技术过程

IF 0.7 Q4 MICROBIOLOGY
Sruthy MS Pillai, R. K. Srivastava, Sujeet Pratap Singh
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引用次数: 0

摘要

当今时代,存在着大量的废弃有机物,给环境造成了巨大负担。为了解决这些问题,研究人员开始应用有效的微生物诱导生物技术过程来减少这些废物。在这种情况下,不同性质的微生物系统参与将废弃有机物水解为可发酵糖的过程。特定的微生物系统,如酿酒酵母(Saccharomyces cerevisiae MTCC 3821)和乙酰丁酸梭菌(Clostridium acetobutylicum),可以很容易地消耗这些糖分,并分别生产出生物乙醇和生物丁醇。在特定培养基中培养酿酒酵母,并在 30°C 下以 150 rpm 的转速旋转培养 72 至 96 小时。不同废料中的乙醇浓度范围为 1.2-1.5 g.L-1。乙醇合成是通过摇瓶实验进行的,在废有机物水解溶液中加入葡萄糖(50 g.L-1)。非葡萄糖培养基产生的乙醇少于 3 g.L-1,但葡萄糖培养基产生的乙醇为 4.5 g.L-1。接着,乙酰丁酸梭菌(Clostridium acetobutylicum)在含有废弃有机物作为唯一碳底物的培养基中生长,pH 值为 7,然后在 35°C 的厌氧条件下培养 72 小时,产生丁醇(0.7 至 1.25 g.L-1)。这项研究工作通过废物减量策略促进了生物燃料的合成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbial Induced Biotechnological Processes for Biofuel Production from Waste Organics Conversion
In the current era there are huge quantities of waste organic matter available, creating a big burden to the environment. To address these issues, researchers started to apply effective and microbial induced biotechnological processes that can mitigate these waste matters. In this context, different nature of microbial systems are involved in hydrolysing the waste organic material into fermentable sugar. These can be easily consumed by specific microbial systems like Saccharomyces cerevisiae MTCC 3821 and Clostridium acetobutylicum that produced bioethanol and biobutanol, respectively. Saccharomyces cerevisiae was cultured in specific media and incubated at rotary shaker with 150 rpm at 30°C for 72 to 96 hours. Ethanol concentrations from different waste matters were found in the range of 1.2-1.5 g.L-1. Ethanol synthesis was done by shake flask experiment with addition of glucose (50 g.L-1) to waste organic hydrolyzed solution. Non-glucose media produced less than 3 g.L-1 ethanol but glucose media produced 4.5 g.L-1. Next, Clostridium acetobutylicum was grown in culture media containing waste organics as sole carbon substrate with pH 7 and then was incubated in anaerobic conditions at 35°C for 72 hours, produced butanol (0.7 to 1.25 g.L-1). This research work promoted biofuels synthesis by keeping a waste mitigation strategy.
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来源期刊
Journal of Pure and Applied Microbiology
Journal of Pure and Applied Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
2.00
自引率
0.00%
发文量
266
审稿时长
11 months
期刊介绍: Journal of Pure and Applied Microbiology (JPAM) is a peer-reviewed, open access international journal of microbiology aims to advance and disseminate research among scientists, academics, clinicians and microbiologists around the world. JPAM publishes high-quality research in all aspects of microbiology in both online and print form on quarterly basis.
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