Residual effect of biochar on microbial biomass and enzyme activities of soil cultivated with grape varieties: a two -year field assessment

Biochar has received increasing attention as a soil conditioner, being known for its ability to confer numerous benefits to the soil. Derived from the thermal decomposition of organic materials under low levels of oxygen, biochar concentrates essential nutrients and carbon while having low carbon dioxide emissions. Our hypothesis is that biochar applied as a top dressing can improve soil microbial attributes even after two years in a soil cultivated with different grape varieties. To test this hypothesis, we conducted a field experiment using different doses of cashew wood biochar applied to two grape varieties and evaluated its residual effect on microbial biomass and soil enzymatic activities. The design used was a randomized block design, in a 4 x 2 factorial scheme, with five replications: four doses of biochar (0, 5, 10 and 15 ton ha-1) and two grape varieties (Cabernet sauvignon and Malbec). Soil collection was carried out at a depth of 0-20 cm, to evaluate total organic carbon, microbial biomass carbon and soil enzymatic activities. Our findings revealed that the effect of grape varieties on soil biological properties was more pronounced than the biochar dose, indicating that there is a small residual effect of biochar doses after 2 years of application. Overall, our study provides important insights into the residual effect of biochar on soil microbial attributes and is considered an ecologically sustainable alternative, as it solves the problem of waste reuse and provides a disposal that brings benefits to soil properties.