含铝和硅的肠吸收剂对两周通宵光照下小鼠胸腺细胞组成的影响

S. M. Miroshnichenko, S. Michurina, I. Ishchenko, L. Rachkovskaya, A. E. Serykh, E. E. Rachkovsky, A. Letyagin
{"title":"含铝和硅的肠吸收剂对两周通宵光照下小鼠胸腺细胞组成的影响","authors":"S. M. Miroshnichenko, S. Michurina, I. Ishchenko, L. Rachkovskaya, A. E. Serykh, E. E. Rachkovsky, A. Letyagin","doi":"10.29413/abs.2024-9.3.24","DOIUrl":null,"url":null,"abstract":"Background. Continuous lighting contributes to the development of desynchronosis, which is stressful for the body. As a result, the normal functioning of the immune system is disrupted, which in turn can shift the physiological balance towards pathology and endotoxemia. It is relevant to develop innovative drugs based on a sorbent matrix, which can be modified with biologically active molecules that extendedly leave the sorbent surface. At the same time, the sorbent retains the properties of a detoxifier, fixing toxic agents on the surface and removing them from the body, which helps restore the internal environment and normalizes the overall reactivity of the body in extreme conditions.The aim. To study the effect of aluminum- and silicon-containing enterosorbent (based on aluminum oxide and polydimethylsiloxane) on the cellular composition of the thymus and the distribution of thymocytes in the organ according to the cell cycling state in C57Bl/6 mice kept under the all-night lighting.Materials and methods. Animals received sorbent (0.665 g per 1 kg of body weight in 200 μl of distilled water) through an intragastric tube once a day for 14 days against the background of continuous lighting. Intact mice and placebo animals composed control group. We used flow cytometry to assess the percentage of CD3hi and CD3low lymphocytes of the thymus, the CD3low/CD3hi ratio, viability and distribution of cells across according to the cell cycling state.Results. Continuous lighting inhibited the differentiation and maturation of young CD3low lymphocytes into mature forms of CD3hi, reduced the proliferation of thymic epithelial cells, and activated apoptosis of lymphocytes and epithelial cells in the organ. The introduction of the sorbent restored the content and viability of young CD3low lymphocytes and contributed to the preservation of the viability and proliferation of thymic epithelial cells.Conclusion. Using an enterosorbent based on aluminum oxide and polydimethylsiloxane under conditions of continuous lighting helps maintain the functional activity of the thymus, preventing its involution, and is advisable against the background of circadian disruption.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"17 2","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The effect of aluminum- and silicon-containing enterosorbent on the thymic cellular composition in mice kept under two-week all-night lighting\",\"authors\":\"S. M. Miroshnichenko, S. Michurina, I. Ishchenko, L. Rachkovskaya, A. E. Serykh, E. E. Rachkovsky, A. Letyagin\",\"doi\":\"10.29413/abs.2024-9.3.24\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background. Continuous lighting contributes to the development of desynchronosis, which is stressful for the body. As a result, the normal functioning of the immune system is disrupted, which in turn can shift the physiological balance towards pathology and endotoxemia. It is relevant to develop innovative drugs based on a sorbent matrix, which can be modified with biologically active molecules that extendedly leave the sorbent surface. At the same time, the sorbent retains the properties of a detoxifier, fixing toxic agents on the surface and removing them from the body, which helps restore the internal environment and normalizes the overall reactivity of the body in extreme conditions.The aim. To study the effect of aluminum- and silicon-containing enterosorbent (based on aluminum oxide and polydimethylsiloxane) on the cellular composition of the thymus and the distribution of thymocytes in the organ according to the cell cycling state in C57Bl/6 mice kept under the all-night lighting.Materials and methods. Animals received sorbent (0.665 g per 1 kg of body weight in 200 μl of distilled water) through an intragastric tube once a day for 14 days against the background of continuous lighting. Intact mice and placebo animals composed control group. We used flow cytometry to assess the percentage of CD3hi and CD3low lymphocytes of the thymus, the CD3low/CD3hi ratio, viability and distribution of cells across according to the cell cycling state.Results. Continuous lighting inhibited the differentiation and maturation of young CD3low lymphocytes into mature forms of CD3hi, reduced the proliferation of thymic epithelial cells, and activated apoptosis of lymphocytes and epithelial cells in the organ. The introduction of the sorbent restored the content and viability of young CD3low lymphocytes and contributed to the preservation of the viability and proliferation of thymic epithelial cells.Conclusion. Using an enterosorbent based on aluminum oxide and polydimethylsiloxane under conditions of continuous lighting helps maintain the functional activity of the thymus, preventing its involution, and is advisable against the background of circadian disruption.\",\"PeriodicalId\":505136,\"journal\":{\"name\":\"Acta Biomedica Scientifica\",\"volume\":\"17 2\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Biomedica Scientifica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.29413/abs.2024-9.3.24\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Biomedica Scientifica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29413/abs.2024-9.3.24","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

背景。持续照明会导致非同步性的发展,对人体造成压力。因此,免疫系统的正常功能受到破坏,这反过来又会使生理平衡转向病理和内毒素血症。以吸附剂基质为基础开发创新药物具有重要意义,这种基质可以用生物活性分子进行修饰,使其能够长时间离开吸附剂表面。与此同时,吸附剂还保留了解毒剂的特性,将有毒物质固定在表面,并将其排出体外,这有助于恢复机体内部环境,使机体在极端条件下的整体反应正常化。研究含铝和硅的肠道吸附剂(基于氧化铝和聚二甲基硅氧烷)对在通宵照明下饲养的 C57Bl/6 小鼠胸腺细胞组成的影响,以及根据细胞循环状态胸腺细胞在器官中的分布。在连续照明的背景下,动物通过胃内管接受吸附剂(每 1 kg 体重 0.665 克,加入 200 μl 蒸馏水中),每天一次,连续 14 天。完整小鼠和安慰剂动物组成对照组。我们使用流式细胞术评估了胸腺中 CD3hi 和 CD3low 淋巴细胞的百分比、CD3low/CD3hi 比值、存活率以及细胞周期状态下各细胞的分布。连续光照抑制了CD3low淋巴细胞向CD3hi成熟型的分化和成熟,减少了胸腺上皮细胞的增殖,激活了器官中淋巴细胞和上皮细胞的凋亡。吸附剂的引入恢复了CD3低幼淋巴细胞的含量和活力,并有助于保护胸腺上皮细胞的活力和增殖。在持续光照条件下使用基于氧化铝和聚二甲基硅氧烷的肠道吸附剂有助于维持胸腺的功能活动,防止其萎缩,在昼夜节律紊乱的背景下是可取的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effect of aluminum- and silicon-containing enterosorbent on the thymic cellular composition in mice kept under two-week all-night lighting
Background. Continuous lighting contributes to the development of desynchronosis, which is stressful for the body. As a result, the normal functioning of the immune system is disrupted, which in turn can shift the physiological balance towards pathology and endotoxemia. It is relevant to develop innovative drugs based on a sorbent matrix, which can be modified with biologically active molecules that extendedly leave the sorbent surface. At the same time, the sorbent retains the properties of a detoxifier, fixing toxic agents on the surface and removing them from the body, which helps restore the internal environment and normalizes the overall reactivity of the body in extreme conditions.The aim. To study the effect of aluminum- and silicon-containing enterosorbent (based on aluminum oxide and polydimethylsiloxane) on the cellular composition of the thymus and the distribution of thymocytes in the organ according to the cell cycling state in C57Bl/6 mice kept under the all-night lighting.Materials and methods. Animals received sorbent (0.665 g per 1 kg of body weight in 200 μl of distilled water) through an intragastric tube once a day for 14 days against the background of continuous lighting. Intact mice and placebo animals composed control group. We used flow cytometry to assess the percentage of CD3hi and CD3low lymphocytes of the thymus, the CD3low/CD3hi ratio, viability and distribution of cells across according to the cell cycling state.Results. Continuous lighting inhibited the differentiation and maturation of young CD3low lymphocytes into mature forms of CD3hi, reduced the proliferation of thymic epithelial cells, and activated apoptosis of lymphocytes and epithelial cells in the organ. The introduction of the sorbent restored the content and viability of young CD3low lymphocytes and contributed to the preservation of the viability and proliferation of thymic epithelial cells.Conclusion. Using an enterosorbent based on aluminum oxide and polydimethylsiloxane under conditions of continuous lighting helps maintain the functional activity of the thymus, preventing its involution, and is advisable against the background of circadian disruption.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信