Leong Yao Shen, N. Muhammad, Hariz Haikal Nasuha, M. F. Abu Bakar, N. Abdullah, Nur Fazira Abdul Rahim
{"title":"萃取肉桂叶以抑制α-葡萄糖苷酶的最佳参数","authors":"Leong Yao Shen, N. Muhammad, Hariz Haikal Nasuha, M. F. Abu Bakar, N. Abdullah, Nur Fazira Abdul Rahim","doi":"10.47836/pjst.32.4.23","DOIUrl":null,"url":null,"abstract":"Cinnamomum verum (kayu manis) is an herb that possesses antidiabetic properties and has been used for the treatment of diabetes traditionally. However, there is insufficient scientific data to provide optimum extraction parameters for C. verum leaves for antidiabetic activities. This research aims to determine the optimum extraction parameters of C. verum leaves towards α-glucosidase inhibition and evaluate the correlation between α-glucosidase inhibition, total phenolic content and DPPH radical scavenging activity. The C. verum was extracted with water through an infusion method under different temperatures (60.0°C, 77.5°C, 95.0°C) and extraction time lengths (10 minutes, 20 minutes, 30 minutes). The optimization process was designed using Design Expert software, which applied the Response Surface Method (RSM) and Central Composite Design (CCD). The analyses conducted were antidiabetic property (α-glucosidase inhibition assay), total phenolic content (TPC) (Folin-Ciocalteu colorimetric method) and antioxidant property (2,2-diphenyl-1-picrylhydrazyl, DPPH radical scavenging activity). Results showed that the obtained optimum extraction parameters for C. verum leaves (81.10°C, 19.54 minutes) exhibited α-glucosidase inhibition (87.30±0.67%), total phenolic content (0.12±0.00 mg GAE/g) and DPPH radical scavenging activity (51.25±0.48%). The α-glucosidase inhibition positively correlates with DPPH radical scavenging activity and total phenolic content. These findings have provided a positive relationship between extraction temperature and extraction time length on antidiabetic properties of C. verum leaf extract.","PeriodicalId":46234,"journal":{"name":"Pertanika Journal of Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimum Parameters for Extraction of Cinnamomum verum Leaves Towards α-Glucosidase Inhibition\",\"authors\":\"Leong Yao Shen, N. Muhammad, Hariz Haikal Nasuha, M. F. Abu Bakar, N. Abdullah, Nur Fazira Abdul Rahim\",\"doi\":\"10.47836/pjst.32.4.23\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Cinnamomum verum (kayu manis) is an herb that possesses antidiabetic properties and has been used for the treatment of diabetes traditionally. However, there is insufficient scientific data to provide optimum extraction parameters for C. verum leaves for antidiabetic activities. This research aims to determine the optimum extraction parameters of C. verum leaves towards α-glucosidase inhibition and evaluate the correlation between α-glucosidase inhibition, total phenolic content and DPPH radical scavenging activity. The C. verum was extracted with water through an infusion method under different temperatures (60.0°C, 77.5°C, 95.0°C) and extraction time lengths (10 minutes, 20 minutes, 30 minutes). The optimization process was designed using Design Expert software, which applied the Response Surface Method (RSM) and Central Composite Design (CCD). The analyses conducted were antidiabetic property (α-glucosidase inhibition assay), total phenolic content (TPC) (Folin-Ciocalteu colorimetric method) and antioxidant property (2,2-diphenyl-1-picrylhydrazyl, DPPH radical scavenging activity). Results showed that the obtained optimum extraction parameters for C. verum leaves (81.10°C, 19.54 minutes) exhibited α-glucosidase inhibition (87.30±0.67%), total phenolic content (0.12±0.00 mg GAE/g) and DPPH radical scavenging activity (51.25±0.48%). The α-glucosidase inhibition positively correlates with DPPH radical scavenging activity and total phenolic content. These findings have provided a positive relationship between extraction temperature and extraction time length on antidiabetic properties of C. verum leaf extract.\",\"PeriodicalId\":46234,\"journal\":{\"name\":\"Pertanika Journal of Science and Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2024-07-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pertanika Journal of Science and Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.47836/pjst.32.4.23\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pertanika Journal of Science and Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47836/pjst.32.4.23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Optimum Parameters for Extraction of Cinnamomum verum Leaves Towards α-Glucosidase Inhibition
Cinnamomum verum (kayu manis) is an herb that possesses antidiabetic properties and has been used for the treatment of diabetes traditionally. However, there is insufficient scientific data to provide optimum extraction parameters for C. verum leaves for antidiabetic activities. This research aims to determine the optimum extraction parameters of C. verum leaves towards α-glucosidase inhibition and evaluate the correlation between α-glucosidase inhibition, total phenolic content and DPPH radical scavenging activity. The C. verum was extracted with water through an infusion method under different temperatures (60.0°C, 77.5°C, 95.0°C) and extraction time lengths (10 minutes, 20 minutes, 30 minutes). The optimization process was designed using Design Expert software, which applied the Response Surface Method (RSM) and Central Composite Design (CCD). The analyses conducted were antidiabetic property (α-glucosidase inhibition assay), total phenolic content (TPC) (Folin-Ciocalteu colorimetric method) and antioxidant property (2,2-diphenyl-1-picrylhydrazyl, DPPH radical scavenging activity). Results showed that the obtained optimum extraction parameters for C. verum leaves (81.10°C, 19.54 minutes) exhibited α-glucosidase inhibition (87.30±0.67%), total phenolic content (0.12±0.00 mg GAE/g) and DPPH radical scavenging activity (51.25±0.48%). The α-glucosidase inhibition positively correlates with DPPH radical scavenging activity and total phenolic content. These findings have provided a positive relationship between extraction temperature and extraction time length on antidiabetic properties of C. verum leaf extract.
期刊介绍:
Pertanika Journal of Science and Technology aims to provide a forum for high quality research related to science and engineering research. Areas relevant to the scope of the journal include: bioinformatics, bioscience, biotechnology and bio-molecular sciences, chemistry, computer science, ecology, engineering, engineering design, environmental control and management, mathematics and statistics, medicine and health sciences, nanotechnology, physics, safety and emergency management, and related fields of study.