油辣木籽蛋白基纳米粒子的超声辅助绿色合成及其物理化学和细胞毒性活性表征

Nanomaterials Pub Date : 2024-07-26 DOI:10.3390/nano14151254
Amany Abd El-Shafy Abd El-Kader Nafeh, Ibrahim Mohamed Abd El-Aleem Mohamed, M. Foda
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引用次数: 0

摘要

辣木籽(Moringa oleifera,M. oleifera)具有全球公认的药用价值,其种子富含优质蛋白质。本研究旨在探索油麻菜种子作为蛋白基纳米颗粒(PBNPs)重要来源的潜力,并首次使用超声波技术对其在人类白血病细胞系(THP-1)中的细胞毒性进行评估。动态光散射(DLS)、透射电子显微镜(TEM)、扫描电子显微镜(SEM)、能量色散 X 射线光谱(EDX)、X 射线衍射(XRD)和傅立叶变换红外光谱(FT-IR)证实了 PBNPs 的性质。从辣木籽饼粉中提取的蛋白质含量高达 54.20%,得到的 PBNPs 平均粒径为 134.3 ± 0.47 nm,Zeta 电位为 -43.15 mV。值得注意的是,我们的研究发现,PBNPs 在高浓度时具有细胞毒性,尤其是对 THP-1 人类白血病细胞系,该细胞系被广泛用于研究免疫调节特性。细胞毒性试验定量证明了 PBNPs 的抑制作用,该试验表明,206.5 μg mL-1 的浓度(对数浓度 2.315)才能抑制 50%的生物活性。总之,我们的研究结果凸显了油橄榄种子作为生态友好型 PBNPs 创新领域的宝贵资源的潜力,它将传统医药应用与当代先进的蛋白质纳米技术相结合。不过,还需要进一步研究以确保其生物相容性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ultrasonication-Assisted Green Synthesis and Physicochemical and Cytotoxic Activity Characterization of Protein-Based Nanoparticles from Moringa oleifera Seeds
Moringa oleifera (M. oleifera) is globally recognized for its medicinal properties and offers high-quality, protein-rich seeds. This study aimed to explore the potential of M. oleifera seeds as a significant source of protein-based nanoparticles (PBNPs) using the ultrasonication technique after desolvation and to evaluate their cytotoxicity in the human leukemia cell line (THP-1) for the first time. The properties of the PBNPs were confirmed by dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR). The extracted protein from moringa seed cake flour had a significant protein content of 54.20%, and the resulting PBNPs had an average size of 134.3 ± 0.47 nm with a robust zeta potential of −43.15 mV. Notably, our study revealed that PBNPs exhibited cytotoxic potential at high concentrations, especially against the THP-1 human leukemia cell line, which is widely used to study immunomodulatory properties. The inhibitory effect of PBNPs was quantitatively evidenced by a cytotoxicity assay, which showed that a concentration of 206.5 μg mL−1 (log conc. 2.315) was required to inhibit 50% of biological activity. In conclusion, our findings highlight the potential of M. oleifera seeds as a valuable resource in the innovative field of eco-friendly PBNPs by combining traditional medicinal applications with contemporary advancements in protein nanotechnology. However, further studies are required to ensure their biocompatibility.
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