尼日利亚贝宁市铜绿假单胞菌和奇异变形杆菌临床分离物中金属-β-内酰胺酶的流行情况

World Journal of Advanced Research and Reviews Pub Date : 2024-07-30 DOI:10.30574/wjarr.2024.23.1.2060

Olabisi Promise Lawal, Ibrahim Ayo Olanipekun, Bukola Ikuejamoye-Omotore, Adedoyin Tinuade Buari, Omowunmi Ramota Gbadamosi, Cynthia Amarachukwu Ibe-Chukwuemeka

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引用次数: 0

摘要

碳青霉烯类是治疗由多重耐药铜绿假单胞菌和神奇变形杆菌引起的严重感染病例的首选药物。然而，这些微生物产生的金属-β-内酰胺酶（MBL）导致了碳青霉烯类耐药性，这是一个全球性威胁。本研究旨在确定尼日利亚贝宁市铜绿假单胞菌和奇异变形杆菌临床分离物中 MBL 的流行率和抗菌药敏感性概况。使用的 354 个铜绿假单胞菌（282 个）和奇异变形杆菌（72 个）的非单一临床分离物是从贝宁市三级医院的各种临床样本中获得的。使用标准微生物学技术对这些分离物进行了鉴定。使用柯比鲍尔盘扩散法进行了抗菌药敏感性测试。使用亚胺培南乙二胺四乙酸联合圆片测试法检测 MBL 的产生。在检测的 354 个临床分离株中，有 115 个（32.48%）产生了 MBL，与奇异变形杆菌相比，铜绿假单胞菌（46.8%）产生耐药分离株的比例明显更高（P=0.0001）。在铜绿假单胞菌产生金属-β-内酰胺酶的分离株中，胸腔积液和脑脊液样本中的流行率较高，分别为 6 株（100%）和 3 株（100%）。这一差异具有统计学意义（P=0.0001）。药敏试验显示，产生β-内酰胺酶的分离株与不产生β-内酰胺酶的分离株相比，对头孢菌素、阿莫西林-克拉维酸、庆大霉素和氟喹诺酮类药物的耐药性较差。据报告，产生 MBL 的铜绿假单胞菌感染率为 46.8%，产生 MBL 的奇异变形杆菌感染率为 12.5%。产生 MBL 酶的分离菌株对抗菌剂的耐药性更强。强烈建议采取措施控制和遏制产生 MBL 的临床分离菌的传播。

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Prevalence of metallo-beta-lactamase in clinical isolates of Pseudomonas aeruginosa and Proteus mirabilis in Benin City, Nigeria

Carbapenems are the prime choice of treatment for severe cases of infections caused by Multi-Drug-Resistant Pseudomonas aeruginosa and Proteus mirabilis. Nevertheless, Metallo-Beta-Lactamase (MBL) production by these organisms has led to carbapenem resistance which is a global threat. This study aimed to determine the prevalence and antimicrobial susceptibility profile of MBL in clinical isolates of Pseudomonas aeruginosa and Proteus mirabilis in Benin City, Nigeria. 354 non monotonous clinical isolates of Pseudomonas aeruginosa (282) and Proteus mirabilis (72) used were obtained from various clinical samples from tertiary hospitals in Benin City. Identification of these isolates were done using standard microbiological techniques. Antimicrobial susceptibility test was performed using Kirby-Bauer disk diffusion method. MBL production was detected using Imipenem Ethylene-Diamine-Tetra-Acetic Acid combined disc test method. Of the total 354 clinical isolates tested, 115 (32.48%) were MBL and the prevalence of this resistant isolates was significantly higher in Pseudomonas aeruginosa (46.8%) compared to Proteus mirabilis (P=0.0001). Among the metallo-beta-lactamase producing isolates of Pseudomonas aeruginosa higher prevalence were reported from Pleural fluid and cerebrospinal fluid samples with 6 (100%) and 3 (100%) respectively. This difference was statistically significant (P=0.0001). Susceptibility testing showed that isolates that produced beta-lactamase demonstrated poorly against cephalosporin, amoxicillin-clavulanate, gentamicin and floroquinones than non-beta- lactamase producers. A prevalence of 46.8% was reported for MBL producing Pseudomonas aeruginosa and 12.5% was reported for MBL producing Proteus mirabilis. Isolates that produced the MBL enzyme were more resistant to antibacterial agents. Measures to control and curb the spread of MBL producing clinical isolates are highly advocated

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World Journal of Advanced Research and Reviews

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