Cynara cardunculus var. sylvestris 中生物活性化合物的抗氧化活性和通便作用

IF 0.7 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Fatma Tajini, Awatef Jelassi, Amal Hamdani, Ali Salem, Ola Abdelhedi, Abid Ouerghui, H. Sebai
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引用次数: 0

摘要

本研究旨在探讨排骨野生荠菜(Cynara sylvestris)酚类提取物的抗氧化和抗便秘活性。经分析的提取物(丙酮、甲醇和水溶液)显示出高水平的酚类化合物和出色的抗氧化活性,IC50 值显著(p ≤0.05),如 DPPH 自由基清除活性(0.84±0.08 - 2.44±0.007 μg/mL)、ABTS 阳离子自由基测试(0.96±0.01- 136.67±6.75 μg/mL)和β-胡萝卜素漂白试验(2.09±0.02-51.12±1.32 μg/mL)。此外,还发现了较高水平的不溶性膳食纤维(56.18 ± 0.91% DW 的中性洗涤纤维)。在 Wistar 大鼠身上进行了体内调查,以探索西印度蘑菇水提取物(CSAE)在促进胃肠道转运和治疗洛哌丁胺(商品名为 Idium)诱导的便秘方面的能力。当 CSAE 的剂量增加时(分别为 75、150 和 300 毫克/千克体重),胃肠道蠕动明显增加了 15%、24.5% 和 32.4%。与阳性对照组(未处理)相比,摄食量、饮水量、粪便数量和重量的增加也与剂量有关。此外,CSAE 还能保护肠道和结肠粘膜中正常的抗氧化酶活性(SOD 和 GPx),使肝脏酶(谷草转氨酶和谷丙转氨酶)和肾脏酶(尿素和肌酐)水平恢复到正常值,从而对氧化应激起到明显的保护作用(p ≤0.05),且与剂量有关。这些结果可以用这种植物中丰富的酚类化合物和不溶性纤维来解释。因此,可以鼓励将其作为抗氧化剂和润肠通便的食品补充剂应用于食品和医药领域。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antioxidant Activities and Laxative Effect of Bioactive Compounds from Cynara cardunculus var. sylvestris
The present study aims to investigate the antioxidant and anti-constipation activities of the derived phenolic extracts in ribs wild cardoon (Cynara sylvestris). Analysed extracts (acetone, methanol and aqueous) exhibited high level of phenolic compounds and excellent antioxidant activities with significant IC50 values (p ≤0.05), as assessed by the DPPH radical-scavenging activity (0.84±0.08 - 2.44±0.007 μg/mL), ABTS cationic radical test (0.96 ±0.01- 136.67±6.75 μg/mL) and β-carotene bleaching assay (2.09±0.02-51.12±1.32 μg/mL). Also, higher levels of insoluble dietary fibers were found (56.18 ± 0.91% DW of neutral detergent fiber). The in vivo investigation was performed on Wistar rats to explore the ability of C. sylvestris aqueous extract (CSAE) in the enhancement of the gastrointestinal transit and the treatment of induced constipation by Loperamide (commercialized as Idium). A significant increase of 15, 24.5 and 32.4% in gastrointestinal motility was recordedwhen doses of CSAE increased (75, 150 and 300 mg/kg b.w, respectively). Food intake, water consumption, number and weight of stools were also increased in a dose-dependent manner, compared to the positive control (untreated). Moreover, CSAE provided significantly (p ≤0.05) and dose-dependently protection against oxidative stress by preserving normal antioxidant enzymes activities (SOD and GPx) in intestinal and colonic mucosa, and resorted hepatic enzymes (AST and ALT) and renal (urea and creatinine) levels to normal values. These results can be explained by the abundance of phenolic compounds and insoluble fibers in this plant. Therefore, its use can be encouraged in alimentary and pharmaceutical applications as antioxidant and laxative food supplements.
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来源期刊
Sains Malaysiana
Sains Malaysiana MULTIDISCIPLINARY SCIENCES-
CiteScore
1.60
自引率
12.50%
发文量
196
审稿时长
3-6 weeks
期刊介绍: Sains Malaysiana is a refereed journal committed to the advancement of scholarly knowledge and research findings of the several branches of science and technology. It contains articles on Earth Sciences, Health Sciences, Life Sciences, Mathematical Sciences and Physical Sciences. The journal publishes articles, reviews, and research notes whose content and approach are of interest to a wide range of scholars. Sains Malaysiana is published by the UKM Press an its autonomous Editorial Board are drawn from the Faculty of Science and Technology, Universiti Kebangsaan Malaysia. In addition, distinguished scholars from local and foreign universities are appointed to serve as advisory board members and referees.
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