生物合成的纳米铜粒子的抗菌活性--使用洋甘菊的甲醇提取物

International Journal for Research in Applied Science and Engineering Technology Pub Date : 2024-07-31 DOI:10.22214/ijraset.2024.63600

Rishav Biswas

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引用次数: 0

摘要

摘要：奥康树叶的甲醇提取物被用作合成铜纳米粒子（CuNPs）的还原剂和稳定剂。这是一种具有成本效益且环保的工艺。用硫酸铜溶液处理欧加木圣洁叶提取物后，形成了稳定的 CuNPs。所形成的 CuNPs 在紫外可见分光光度计下进行了表征。生物合成的纳米铜粒子对机会性病原体金黄色葡萄球菌具有很强的抗菌活性。抗菌活性是通过琼脂井扩散、最低抑菌浓度（MIC）和最低杀菌浓度（MBC）这三种测定方法来确定的，并通过数值比较来观察 CuNPs 的抗菌效果。

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Antimicrobial Activity of Biosynthesized Copper Nanoparticles Using Methanolic Extract of Ocimum Sanctum

Abstract: Methanolic extract of Ocimum sanctum leaves were used as a reducing and stabilizing agent for the synthesis of copper nanoparticles (CuNPs). It is a cost-effective and eco-friendly process. On the treatment of Ocimum sanctum leaf extract with copper sulphate solution, stable CuNPs were formed. The formed CuNPs were characterized under UV-Vis spectrophotometer. The biologically synthesized copper nanoparticles show high antibacterial activity against opportunistic pathogen Staphylococcus aureus. The antimicrobial activity was determined by three assays with agar well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) and the values were compared to observe the antimicrobial efficacy of the CuNPs.

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International Journal for Research in Applied Science and Engineering Technology

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