Piotr Gruber, Patrycja Szymczyk-Ziółkowska, Michał Olejarczyk, Adam Junka, Krystyna Fabianowska-Majewska, Malwina Brożyna, Tomasz Kurzynowski
{"title":"生物活性金属填料对激光粉末床融合聚合物生产的 PA12 复合材料抗菌性能的影响。","authors":"Piotr Gruber, Patrycja Szymczyk-Ziółkowska, Michał Olejarczyk, Adam Junka, Krystyna Fabianowska-Majewska, Malwina Brożyna, Tomasz Kurzynowski","doi":"10.37190/abb-02303-2023-04","DOIUrl":null,"url":null,"abstract":"<p><p><i>Purpose</i>: This study investigated the influence of three types of metallic microfillers, spherical silver and spherical, and dendritic copper, on the ability of polyamide 12 (PA12) to inhibit microorganism growth on the surfaces of samples produced using laser-based powder bed fusion of polymers (PBF-LB/P). The aim of this study was to initially characterize these materials regarding their potential applicability for parts dedicated to use in the hospitals, where surfaces are periodically disinfected using chemical and/or physical measures. <i>Methods</i>: Composite powders with filler concentrations of 0.5, 1, 2 and 5% by weight were prepared using the mechanical mixing method and processed using PBF-LB/P. Three common hospital pathogens responsible for healthcare-associated infections: <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i> and <i>Candida albicans</i> were tested. Additionally, the safety of the composites was assessed through <i>in vitro</i> tests using human cell lines: keratinocytes and fibroblasts. <i>Results</i>: The research reveals that addition of copper or silver causes decrease in bacterial colony viability compared to the material without a filler, but an insignificant effect on antifungal properties. There was no significant impact within the tested range of filler's content on the antibacterial properties. Furthermore, a strong effect of the microfillers on tested material's toxicity is observed. <i>Conclusions</i>: The addition of metallic microfillers enhances the antibacterial response of polymeric materials processed with PBF-LB/P. Nevertheless, the observed varying levels of cytotoxicity toward eukaryotic cell lines underscore the need for further studies on the analysed materials to unequivocally determine their potential applicability as materials for short-term contact with human skin in a hospital setting.</p>","PeriodicalId":519996,"journal":{"name":"Acta of bioengineering and biomechanics","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Influence of bioactive metal fillers on antimicrobial properties of PA12 composites produced by laser-based powder bed fusion of polymers.\",\"authors\":\"Piotr Gruber, Patrycja Szymczyk-Ziółkowska, Michał Olejarczyk, Adam Junka, Krystyna Fabianowska-Majewska, Malwina Brożyna, Tomasz Kurzynowski\",\"doi\":\"10.37190/abb-02303-2023-04\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Purpose</i>: This study investigated the influence of three types of metallic microfillers, spherical silver and spherical, and dendritic copper, on the ability of polyamide 12 (PA12) to inhibit microorganism growth on the surfaces of samples produced using laser-based powder bed fusion of polymers (PBF-LB/P). The aim of this study was to initially characterize these materials regarding their potential applicability for parts dedicated to use in the hospitals, where surfaces are periodically disinfected using chemical and/or physical measures. <i>Methods</i>: Composite powders with filler concentrations of 0.5, 1, 2 and 5% by weight were prepared using the mechanical mixing method and processed using PBF-LB/P. Three common hospital pathogens responsible for healthcare-associated infections: <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i> and <i>Candida albicans</i> were tested. Additionally, the safety of the composites was assessed through <i>in vitro</i> tests using human cell lines: keratinocytes and fibroblasts. <i>Results</i>: The research reveals that addition of copper or silver causes decrease in bacterial colony viability compared to the material without a filler, but an insignificant effect on antifungal properties. There was no significant impact within the tested range of filler's content on the antibacterial properties. Furthermore, a strong effect of the microfillers on tested material's toxicity is observed. <i>Conclusions</i>: The addition of metallic microfillers enhances the antibacterial response of polymeric materials processed with PBF-LB/P. Nevertheless, the observed varying levels of cytotoxicity toward eukaryotic cell lines underscore the need for further studies on the analysed materials to unequivocally determine their potential applicability as materials for short-term contact with human skin in a hospital setting.</p>\",\"PeriodicalId\":519996,\"journal\":{\"name\":\"Acta of bioengineering and biomechanics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta of bioengineering and biomechanics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.37190/abb-02303-2023-04\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/12/1 0:00:00\",\"PubModel\":\"Print\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta of bioengineering and biomechanics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37190/abb-02303-2023-04","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/12/1 0:00:00","PubModel":"Print","JCR":"","JCRName":"","Score":null,"Total":0}
Influence of bioactive metal fillers on antimicrobial properties of PA12 composites produced by laser-based powder bed fusion of polymers.
Purpose: This study investigated the influence of three types of metallic microfillers, spherical silver and spherical, and dendritic copper, on the ability of polyamide 12 (PA12) to inhibit microorganism growth on the surfaces of samples produced using laser-based powder bed fusion of polymers (PBF-LB/P). The aim of this study was to initially characterize these materials regarding their potential applicability for parts dedicated to use in the hospitals, where surfaces are periodically disinfected using chemical and/or physical measures. Methods: Composite powders with filler concentrations of 0.5, 1, 2 and 5% by weight were prepared using the mechanical mixing method and processed using PBF-LB/P. Three common hospital pathogens responsible for healthcare-associated infections: Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans were tested. Additionally, the safety of the composites was assessed through in vitro tests using human cell lines: keratinocytes and fibroblasts. Results: The research reveals that addition of copper or silver causes decrease in bacterial colony viability compared to the material without a filler, but an insignificant effect on antifungal properties. There was no significant impact within the tested range of filler's content on the antibacterial properties. Furthermore, a strong effect of the microfillers on tested material's toxicity is observed. Conclusions: The addition of metallic microfillers enhances the antibacterial response of polymeric materials processed with PBF-LB/P. Nevertheless, the observed varying levels of cytotoxicity toward eukaryotic cell lines underscore the need for further studies on the analysed materials to unequivocally determine their potential applicability as materials for short-term contact with human skin in a hospital setting.