通过转录组比较分析揭示回交近交系水稻(Oryza sativa L. )的抗旱分子机制

IF 1.5 4区 农林科学 Q2 AGRONOMY
Plant Breeding Pub Date : 2024-07-22 DOI:10.1111/pbr.13208
Kari Baghyalakshmi, Selvaraj Ramchander, Nallathambi Jagadeeshselvam, Muthurajan Raveendran, Paramasiwam Jeyaprakash
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引用次数: 0

摘要

干旱胁迫严重阻碍了水稻生产,造成了巨大的经济损失。全球气候变化问题日益重要,因此开发耐干旱胁迫的水稻基因型变得越来越关键。为解决这一问题,我们对所培育的回交近交系(BILs)进行了干旱胁迫及其分子机理研究。耐旱亲本 Apo 和易旱高产 IR64 以及两个 BIL,即 CB 229(qDTY2.2 + qDTY3.1 + qDTY8.1)和 CB 193-3(qDTY3.1 + qDTY8.1),在温室中进行了干旱响应测试。在这项研究中,CB 229 在水胁迫灌溉条件下表现更好,与 IR64 相当。通过 RNA-Seq 方法,对亲本和优良的 BIL CB 229 进行了干旱响应转录组分析。在耐旱性 BIL CB 229 中检测到约 3050 个差异表达基因(DEGs)(2021 个上调,1029 个下调)。大多数 DEGs 参与了碳水化合物代谢和细胞壁的形成,以及与代谢物适应性、ROS 平衡和转录后调控相关的基因。在耐受性 Apo 和 CB 229 中,伴侣蛋白、衰老诱导受体样丝氨酸、6-磷酸甘露糖异构酶、水蒸蛋白和热休克蛋白(LOC_Os02g26840、LOC_Os02g25720、LOC_Os07g35570、LOC_Os01g64970 等)等基因上调。据观察,在水分胁迫下,BIL CB 229 的谷粒产量高于亲本。在 CB 229、Apo 和 IR64 中,发现数量性状位点(QTLs)区域的 94 个基因受到不同调控。在这94个基因中,共定位在QTL qDTY2.2中的9个基因、qDTY3.1中的12个基因和qDTY8.1中的4个基因在CB 229中被上调,而在易感基因型中被下调。研究发现,qDTY2.2、qDTY3.1 和 qDTY8.1 QTLs 具有互补效应,由于互补作用,它们对干旱的耐受性增强。此外,这项分析还发现了一些新的 DEGs,它们可能参与了与耐旱性相关的功能,但缺乏功能注释。未来的研究可以重点关注本研究中发现的几个重要 DEGs。总之,我们的研究结果提供了对耐旱机制的深入了解,有助于开发新的策略来提高水稻的耐旱性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative transcriptome profiling to untie the drought tolerance molecular mechanism of backcross rice (Oryza sativa L.) inbred
Rice production is severely hampered by drought stress, which causes enormous economic losses. The issue of global climate change is gaining importance, and hence development of rice genotypes tolerant to drought stress is becoming more critical. To address this issue, backcross inbred lines (BILs) developed were subjected to drought stress, and their molecular mechanism was studied. The drought‐tolerant parent Apo and drought‐susceptible, high‐yielding IR64 along with two BILs, namely, CB 229 (qDTY2.2 + qDTY3.1 + qDTY8.1) and CB 193‐3 (qDTY3.1 + qDTY8.1) were tested in a greenhouse for their response to drought. In this study, CB 229 showed better performance under water stress irrigated conditions; it was on par with IR64. Drought‐responsive transcriptome profiling was carried out in both the parents and the superior BIL CB 229 through the RNA‐Seq approach. About 3050 differentially expressed genes (DEGs) (2021 upregulated and 1029 downregulated) were detected in tolerant BIL CB 229 in drought stress. Most of the DEGs were involved in carbohydrate metabolism and the formation of cell walls, as well as genes associated with metabolite adaptability, ROS homeostasis and post‐transcriptional regulation. Genes such as chaperone protein, senescence‐induced receptor‐like serine, mannose‐6‐phosphate isomerase, aquaporin and heat shock proteins (LOC_Os02g26840, LOC_Os02g25720, LOC_Os07g35570, LOC_Os01g64970, etc.) were upregulated in the tolerant Apo and CB 229. It was observed that the BIL CB 229 yielded higher grains than both parents under moisture stress. Ninety‐four genes in the quantitative trait loci (QTLs) region were found to be differentially regulated in CB 229, Apo and IR64. Out of 94, nine genes co‐localized within the QTL qDTY2.2, 12 genes within qDTY3.1 and four genes within qDTY8.1 were differentially upregulated in CB 229 and downregulated in the susceptible genotype. The study revealed that the QTLs qDTY2.2, qDTY3.1 and qDTY8.1 are found to have complementary effects and offer enhanced levels of tolerance against drought due to complementation. Additionally, this analysis discovered new DEGs that may be involved in functions related to drought tolerance but lack function annotations. Future research can focus on a few of the significant DEGs found in this study. Taken together, our findings provide insight into drought tolerance mechanisms and could assist the development of novel strategies for improving drought tolerance in rice.
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来源期刊
Plant Breeding
Plant Breeding 农林科学-农艺学
CiteScore
4.40
自引率
5.00%
发文量
74
审稿时长
3.0 months
期刊介绍: PLANT BREEDING publishes full-length original manuscripts and review articles on all aspects of plant improvement, breeding methodologies, and genetics to include qualitative and quantitative inheritance and genomics of major crop species. PLANT BREEDING provides readers with cutting-edge information on use of molecular techniques and genomics as they relate to improving gain from selection. Since its subject matter embraces all aspects of crop improvement, its content is sought after by both industry and academia. Fields of interest: Genetics of cultivated plants as well as research in practical plant breeding.
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