Maria Breygina, Oksana Luneva, Ksenia Babushkina, Olga Schekaleva, Svetlana Polevova
{"title":"针叶植物授粉滴中的活性氧","authors":"Maria Breygina, Oksana Luneva, Ksenia Babushkina, Olga Schekaleva, Svetlana Polevova","doi":"10.1007/s40626-024-00343-2","DOIUrl":null,"url":null,"abstract":"<p>In flowering plants, reactive oxygen species (ROS) are actively involved in the regulation of sexual reproduction. Here we present the first evidence on the participation of ROS in sexual interactions in coniferous plants. In most gymnosperms, pollen hydrates and germinates in a pollination drop—a special type of ovular secretion. We studied the composition of this liquid for four conifer species from three families for the presence of ROS using the most sensitive method for detecting low concentrations of ROS—EPR (electron paramagnetic resonance) spectroscopy. ROS were present in pollination drops of all the plants studied, however, the composition of ROS was different: in <i>Taxus</i>, <i>Pinus</i> and <i>Picea</i> the main form was O<sup>⋅</sup><sub>2</sub><sup>−</sup>, in <i>Thuja</i> it was H<sub>2</sub>O<sub>2</sub>, reaching values comparable to stigma exudates of flowering plants. We tested the potential involvement of superoxide in the regulation of spruce pollen germination in vitro and found a strong stimulating effect on both total and bipolar germination efficiency. Thus, we found that conifers are characterized by the presence of ROS in the liquid intended for pollen germination, as well as in flowering plants. Hydrogen peroxide and superoxide radical, apparently represent a variety of ROS-based mechanisms for the regulation of pollen germination in vivo.</p>","PeriodicalId":23038,"journal":{"name":"Theoretical and Experimental Plant Physiology","volume":"54 47 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Reactive oxygen species in pollination drops of coniferous plants\",\"authors\":\"Maria Breygina, Oksana Luneva, Ksenia Babushkina, Olga Schekaleva, Svetlana Polevova\",\"doi\":\"10.1007/s40626-024-00343-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>In flowering plants, reactive oxygen species (ROS) are actively involved in the regulation of sexual reproduction. Here we present the first evidence on the participation of ROS in sexual interactions in coniferous plants. In most gymnosperms, pollen hydrates and germinates in a pollination drop—a special type of ovular secretion. We studied the composition of this liquid for four conifer species from three families for the presence of ROS using the most sensitive method for detecting low concentrations of ROS—EPR (electron paramagnetic resonance) spectroscopy. ROS were present in pollination drops of all the plants studied, however, the composition of ROS was different: in <i>Taxus</i>, <i>Pinus</i> and <i>Picea</i> the main form was O<sup>⋅</sup><sub>2</sub><sup>−</sup>, in <i>Thuja</i> it was H<sub>2</sub>O<sub>2</sub>, reaching values comparable to stigma exudates of flowering plants. We tested the potential involvement of superoxide in the regulation of spruce pollen germination in vitro and found a strong stimulating effect on both total and bipolar germination efficiency. Thus, we found that conifers are characterized by the presence of ROS in the liquid intended for pollen germination, as well as in flowering plants. Hydrogen peroxide and superoxide radical, apparently represent a variety of ROS-based mechanisms for the regulation of pollen germination in vivo.</p>\",\"PeriodicalId\":23038,\"journal\":{\"name\":\"Theoretical and Experimental Plant Physiology\",\"volume\":\"54 47 1\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-07-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Theoretical and Experimental Plant Physiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s40626-024-00343-2\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Theoretical and Experimental Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s40626-024-00343-2","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Reactive oxygen species in pollination drops of coniferous plants
In flowering plants, reactive oxygen species (ROS) are actively involved in the regulation of sexual reproduction. Here we present the first evidence on the participation of ROS in sexual interactions in coniferous plants. In most gymnosperms, pollen hydrates and germinates in a pollination drop—a special type of ovular secretion. We studied the composition of this liquid for four conifer species from three families for the presence of ROS using the most sensitive method for detecting low concentrations of ROS—EPR (electron paramagnetic resonance) spectroscopy. ROS were present in pollination drops of all the plants studied, however, the composition of ROS was different: in Taxus, Pinus and Picea the main form was O⋅2−, in Thuja it was H2O2, reaching values comparable to stigma exudates of flowering plants. We tested the potential involvement of superoxide in the regulation of spruce pollen germination in vitro and found a strong stimulating effect on both total and bipolar germination efficiency. Thus, we found that conifers are characterized by the presence of ROS in the liquid intended for pollen germination, as well as in flowering plants. Hydrogen peroxide and superoxide radical, apparently represent a variety of ROS-based mechanisms for the regulation of pollen germination in vivo.
期刊介绍:
The journal does not publish articles in taxonomy, anatomy, systematics and ecology unless they have a physiological approach related to the following sections:
Biochemical Processes: primary and secondary metabolism, and biochemistry;
Photobiology and Photosynthesis Processes;
Cell Biology;
Genes and Development;
Plant Molecular Biology;
Signaling and Response;
Plant Nutrition;
Growth and Differentiation: seed physiology, hormonal physiology and photomorphogenesis;
Post-Harvest Physiology;
Ecophysiology/Crop Physiology and Stress Physiology;
Applied Plant Ecology;
Plant-Microbe and Plant-Insect Interactions;
Instrumentation in Plant Physiology;
Education in Plant Physiology.