Jianjun Wang, Ruochen Zhang, Hongshan Deng, Xueqin Han, Xuekai Wei, Malik Kamran, James F. White, Guiqin Zhao, Chunjie Li
{"title":"Pseudomonas syringae pv. coronafaciens, causing a new bacterial halo blight disease on oat (Avena sativa L.) in China","authors":"Jianjun Wang, Ruochen Zhang, Hongshan Deng, Xueqin Han, Xuekai Wei, Malik Kamran, James F. White, Guiqin Zhao, Chunjie Li","doi":"10.1007/s10658-024-02920-2","DOIUrl":null,"url":null,"abstract":"<p><i>Pseudomonas syringae</i> pv. <i>coronafaciens</i> (<i>Ps-c</i>) is a pathogenic bacterium species that can be found in agricultural environments and causes diseases in plants. During a survey in 2019–2020 of different areas, bacterial halo blight (BHB) caused by <i>Ps-c</i> in oat (<i>Avena sativa</i> L.) was observed in many oat fields in Huan county, Gansu Province of China. Average leaf disease incidence was approximately 1 to 14%. The symptoms of BHB were mainly observed on leaves of oat and appeared as white to yellow streaks. After 20–30 d, the streaks became yellow necrotic lesions and spread over whole leaves, eventually causing leaves to wilt and senesce. Symptomatic leaves of affected oats were collected to investigate the etiology of BHB disease. A group of 32 bacterial isolates (HK7) phenotypically similar were isolated from the necrotic lesion margins. Pathogenicity tests of this group of isolates by wounding and injection demonstrated that the symptoms induced by them on oat were similar to those observed in the field. HK7 isolates were identified as <i>Ps-c</i> based on Biolog Gen III MicroStation, morphological, physiological and biochemical characterization, 16S rRNA and <i>gyr</i>B gene sequence analysis, which compared with a reference strain (<i>Ps-c</i> ATCC 19608). Furthermore, <i>Ps-c</i> was host specific. After inoculation of <i>Ps-c</i> on <i>A. sativa</i> (B7) and <i>A. nuda</i> (B2) in greenhouse by Completely Randomized Design (CRD), number of tillers, plant height, flag-leaf length, flag-leaf width, fresh weight, dry weight, panicle length, grains per spike, reproductive branche number and thousand grains weight of infected oat decreased by 19.44%, 11.49%, 11.34%, 9.23%, 11.61%, 7.57%, 6.11%, 9.73%, 9.31% and 4.15% compared with that of healthy oat, respectively. BHB (<i>Ps-c</i>) is a new bacterial disease on oat in China and has a negative effect on plant growth, forage and grain yield of oat, and these negative effects increased with the increase of disease severity.</p>","PeriodicalId":12052,"journal":{"name":"European Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pseudomonas syringae pv. coronafaciens, causing a new bacterial halo blight disease on oat (Avena sativa L.) in China\",\"authors\":\"Jianjun Wang, Ruochen Zhang, Hongshan Deng, Xueqin Han, Xuekai Wei, Malik Kamran, James F. White, Guiqin Zhao, Chunjie Li\",\"doi\":\"10.1007/s10658-024-02920-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Pseudomonas syringae</i> pv. <i>coronafaciens</i> (<i>Ps-c</i>) is a pathogenic bacterium species that can be found in agricultural environments and causes diseases in plants. During a survey in 2019–2020 of different areas, bacterial halo blight (BHB) caused by <i>Ps-c</i> in oat (<i>Avena sativa</i> L.) was observed in many oat fields in Huan county, Gansu Province of China. Average leaf disease incidence was approximately 1 to 14%. The symptoms of BHB were mainly observed on leaves of oat and appeared as white to yellow streaks. After 20–30 d, the streaks became yellow necrotic lesions and spread over whole leaves, eventually causing leaves to wilt and senesce. Symptomatic leaves of affected oats were collected to investigate the etiology of BHB disease. A group of 32 bacterial isolates (HK7) phenotypically similar were isolated from the necrotic lesion margins. Pathogenicity tests of this group of isolates by wounding and injection demonstrated that the symptoms induced by them on oat were similar to those observed in the field. HK7 isolates were identified as <i>Ps-c</i> based on Biolog Gen III MicroStation, morphological, physiological and biochemical characterization, 16S rRNA and <i>gyr</i>B gene sequence analysis, which compared with a reference strain (<i>Ps-c</i> ATCC 19608). Furthermore, <i>Ps-c</i> was host specific. After inoculation of <i>Ps-c</i> on <i>A. sativa</i> (B7) and <i>A. nuda</i> (B2) in greenhouse by Completely Randomized Design (CRD), number of tillers, plant height, flag-leaf length, flag-leaf width, fresh weight, dry weight, panicle length, grains per spike, reproductive branche number and thousand grains weight of infected oat decreased by 19.44%, 11.49%, 11.34%, 9.23%, 11.61%, 7.57%, 6.11%, 9.73%, 9.31% and 4.15% compared with that of healthy oat, respectively. 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引用次数: 0
摘要
Pseudomonas syringae pv. coronafaciens（Ps-c）是一种可在农业环境中发现并引起植物病害的病原菌。在2019-2020年对不同地区的调查中，中国甘肃省环县的许多燕麦田都观察到了由Ps-c引起的燕麦（Avena sativa L.）细菌性晕斑病（BHB）。叶片病害的平均发生率约为 1%至 14%。BHB 的症状主要出现在燕麦叶片上，表现为白色至黄色条纹。20-30 d 后，条纹变为黄色坏死病斑并蔓延至整个叶片，最终导致叶片枯萎和衰老。为了研究 BHB 病的病因，我们收集了受害燕麦的症状叶片。从坏死病变边缘分离出一组 32 个表型相似的细菌分离物（HK7）。通过伤口和注射对这组分离物进行的致病性测试表明，它们在燕麦上诱发的症状与在田间观察到的症状相似。根据 Biolog Gen III MicroStation、形态学、生理学和生物化学特征、16S rRNA 和 gyrB 基因序列分析，并与参考菌株（Ps-c ATCC 19608）进行比较，确定 HK7 分离物为 Ps-c。此外，Ps-c 还具有宿主特异性。通过完全随机设计（CRD）将 Ps-c 接种到温室中的 A. sativa（B7）和 A. nuda（B2）上后，受感染燕麦的分蘖数、株高、旗叶长、旗叶宽、鲜重、干重、圆锥花序长、每穗粒数、生殖分枝数和千粒重分别减少了 19.44%、11.49%、11.49% 和 19.44%。与健康燕麦相比，分别减少了 19.44%、11.49%、11.34%、9.23%、11.61%、7.57%、6.11%、9.73%、9.31% 和 4.15%。BHB（Ps-c）是中国燕麦上一种新的细菌性病害，对燕麦的植株生长、饲料和谷物产量均有负面影响，且这些负面影响随着病害严重程度的增加而增加。
Pseudomonas syringae pv. coronafaciens, causing a new bacterial halo blight disease on oat (Avena sativa L.) in China
Pseudomonas syringae pv. coronafaciens (Ps-c) is a pathogenic bacterium species that can be found in agricultural environments and causes diseases in plants. During a survey in 2019–2020 of different areas, bacterial halo blight (BHB) caused by Ps-c in oat (Avena sativa L.) was observed in many oat fields in Huan county, Gansu Province of China. Average leaf disease incidence was approximately 1 to 14%. The symptoms of BHB were mainly observed on leaves of oat and appeared as white to yellow streaks. After 20–30 d, the streaks became yellow necrotic lesions and spread over whole leaves, eventually causing leaves to wilt and senesce. Symptomatic leaves of affected oats were collected to investigate the etiology of BHB disease. A group of 32 bacterial isolates (HK7) phenotypically similar were isolated from the necrotic lesion margins. Pathogenicity tests of this group of isolates by wounding and injection demonstrated that the symptoms induced by them on oat were similar to those observed in the field. HK7 isolates were identified as Ps-c based on Biolog Gen III MicroStation, morphological, physiological and biochemical characterization, 16S rRNA and gyrB gene sequence analysis, which compared with a reference strain (Ps-c ATCC 19608). Furthermore, Ps-c was host specific. After inoculation of Ps-c on A. sativa (B7) and A. nuda (B2) in greenhouse by Completely Randomized Design (CRD), number of tillers, plant height, flag-leaf length, flag-leaf width, fresh weight, dry weight, panicle length, grains per spike, reproductive branche number and thousand grains weight of infected oat decreased by 19.44%, 11.49%, 11.34%, 9.23%, 11.61%, 7.57%, 6.11%, 9.73%, 9.31% and 4.15% compared with that of healthy oat, respectively. BHB (Ps-c) is a new bacterial disease on oat in China and has a negative effect on plant growth, forage and grain yield of oat, and these negative effects increased with the increase of disease severity.
期刊介绍：
The European Journal of Plant Pathology is an international journal publishing original articles in English dealing with fundamental and applied aspects of plant pathology; considering disease in agricultural and horticultural crops, forestry, and in natural plant populations. The types of articles published are :Original Research at the molecular, physiological, whole-plant and population levels; Mini-reviews on topics which are timely and of global rather than national or regional significance; Short Communications for important research findings that can be presented in an abbreviated format; and Letters-to-the-Editor, where these raise issues related to articles previously published in the journal. Submissions relating to disease vector biology and integrated crop protection are welcome. However, routine screenings of plant protection products, varietal trials for disease resistance, and biological control agents are not published in the journal unless framed in the context of strategic approaches to disease management.
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