丙氨酸氨基转移酶和天冬氨酸氨基转移酶检测试剂中吡哆醛-5'-磷酸补充剂的认知和使用报告:美国病理学家学会临床化学委员会调查。

Allison B Chambliss, Rhona J Souers, Jonathan R Genzen, David M Manthei
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引用次数: 0

摘要

背景丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)检测试剂既可添加也可不添加 5'-磷酸吡哆醛(P5P;维生素 B6 的活性形式),P5P 是这两种酶反应所需的催化辅助因子。不补充维生素 B6 的检测方法可能会漏检维生素 B6 缺乏症患者的谷丙转氨酶或谷草转氨酶升高:评估对添加 P5P 的 ALT 和 AST 试剂的认识和采用情况:美国病理学家学会普通化学和治疗药物(C 计划)能力验证 2023 B 邮件中包括一项关于 ALT 和 AST 试剂补充 P5P 的 4 个问题的调查:总体而言,38%(4304 个实验室中的 1651 个)做出回应的实验室报告使用了添加 P5P 的谷丙转氨酶和/或谷草转氨酶试剂。非学术性医院/医疗中心实验室(44%;1629 个实验室中的 713 个)相对于其他环境更常使用 P5P 补充剂。在报告未使用 P5P 补充试剂的实验室中,很少有实验室(5%;2611 家实验室中的 141 家)表示计划在未来进行转换。尽管几家主要的检测试剂生产商都有 P5P 补充试剂,但最常见的使用障碍是实验室的试剂生产商不提供 P5P 补充试剂:结论:人们对添加 P5P 的谷丙转氨酶和谷草转氨酶试剂的存在和益处缺乏认识。ALT 和 AST 检测试剂制造商有必要明确并规范 ALT 和 AST 试剂的 P5P 状态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Reported Awareness and Use of Pyridoxal-5'-Phosphate Supplementation in Alanine Aminotransferase and Aspartate Aminotransferase Assay Reagents: A Survey by the College of American Pathologists Clinical Chemistry Committee.

Context.—: Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) assay reagents are available both with and without supplementation with pyridoxal-5'-phosphate (P5P; the active form of vitamin B6), a catalytic cofactor required for their enzymatic reactions. Nonsupplemented assays may miss ALT or AST elevations in patients with vitamin B6 deficiency.

Objective.—: To assess awareness and adoption of ALT and AST reagents that are supplemented with P5P.

Design.—: A 4-question survey about ALT and AST reagent supplementation with P5P was included in the College of American Pathologists General Chemistry and Therapeutic Drugs (C program) proficiency testing 2023 B mailing.

Results.—: Overall, 38% (1651 of 4304) of responding laboratories reported using ALT and/or AST reagent supplemented with P5P. P5P supplementation was more common for nonacademic hospital/medical center laboratories (44%; 713 of 1629) relative to other settings. Of the laboratories that reported not using P5P-supplemented reagents, few (5%; 141 of 2611) cited plans to convert in the future. Despite the availability of P5P-supplemented reagents from several major assay manufacturers, the most common stated barrier for adoption was that the laboratory's reagent manufacturer does not provide P5P-supplemented reagents.

Conclusions.—: There is a lack of awareness of the existence and benefits of P5P-supplemented ALT and AST reagents. There is a need for ALT and AST assay manufacturers to clarify and standardize the P5P status of ALT and AST reagents.

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