一组新的红豆杉属叶绿体 SSR 标记揭示了种内差异

IF 1.1 4区农林科学 Q3 FORESTRY

Silvae Genetica Pub Date : 2024-07-22 DOI:10.2478/sg-2024-0012

Jeremias Götz, Oliver Caré, Winston Beck, Oliver Gailing, Bernhard Hosius, Ludger Leinemann

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引用次数: 0

摘要

胡桃属（Juglans）是分布于世界各地的重要经济树种。由于其价值很高，人们对用于种群遗传分析和繁殖材料认证的遗传标记很感兴趣。我们利用不同胡桃品种的叶绿体基因组，开发了一套由 11 个 cpSSR 组成的高多态性和可靠的叶绿体 SSR 标记。我们在五个不同的红豆杉物种中成功地进行了测试。评估了物种之间和物种内部产地之间的遗传差异。通过使用新型 cpSSR 标记，我们总共确定了九种不同的叶绿体单倍型，在一个胡颓子物种中最多可有四种不同的单倍型。与全叶绿体基因组测序或 SNP 分析等其他可用方法相比，SSR 标记具有成本低、省力的特点，因此这套新型 cpSSR 标记将有助于未来的胡桃属种群遗传研究。

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A Novel Set of Chloroplast SSR Markers for the Genus Juglans Reveals Within Species Differentiation

Juglans is an economically important genus distributed all over the world. Due to its high value, there is a large interest in genetic markers for population genetic analyses and the certification of reproductive material. Using chloroplast genomes of different Juglans species, we developed a highly polymorphic and reliable chloroplast SSR marker set of eleven cpSSRs. It was successfully tested in five different Juglans species. Genetic differentiation among species and provenances within species was assessed. We were able to define a total of nine different chloroplast haplotypes using the novel cpSSR markers with up to four different haplotypes within a single Juglans species. Due to the cost and labor effective character of SSR markers, when compared to other available methods, such as whole chloroplast genome sequencing or SNP analyses, this novel cpSSR marker set will contribute to future population genetic studies in the Juglans genus.

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来源期刊

Silvae Genetica 农林科学-林学

CiteScore

2.20

自引率

10.00%

发文量

审稿时长

3 months

期刊介绍： Silvae Genetica is an international peer reviewed journal with more than 65 year tradition and experience in all fields of theoretical and applied Forest Genetics and Tree breeding. It continues "Zeitschrift für Forstgenetik und Forstpflanzenzüchtung" (Journal of Forest Genetics and Forest Tree Breeding) founded by W. LANGNER in 1951.