紫檀芪通过抑制 KANK3 水平发挥抗肺部鳞状细胞癌的作用。

IF 3.2 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hua He, Tian Li
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引用次数: 0

摘要

肺鳞状细胞癌(LUSC)的早期检测对临床疗效有重要影响,而紫檀芪(PT)是一种具有良好抗肿瘤活性的天然化合物。本研究旨在通过一系列生物信息学分析和临床验证,确定潜在的LUSC生物标志物,并探讨PT与所选生物标志物在LUSC治疗过程中的相互作用。该研究对LUSC临床样本的表达谱进行了分析,以确定低表达基因(DEGs),并通过IHC进行验证。通过一系列体外和体内试验评估了KANK3在PT抗LUSC效应中的作用。共鉴定出4335个DEGs,包括1851个上调基因和2484个下调基因。生存分析表明,KANK3在肿瘤晚期的LUSC患者中明显升高。在体外实验中,PT能抑制LUSC细胞株的细胞活力、诱导细胞凋亡、抑制迁移和侵袭,这与KANK3的下调有关。在 LUSC 细胞中重新恢复 KANK3 水平后,PT 的抗 LUSC 功能受损。在小鼠模型中,即使在 PT 的治疗下,KANK3 的重新恢复也会增加肿瘤的生长和转移。本研究概述的结果表明,PT以一种依赖于KANK3抑制的方式发挥抗LUSC功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Pterostilbene exerts anti-lung squamous cell carcinoma function by suppressing the level of KANK3

Pterostilbene exerts anti-lung squamous cell carcinoma function by suppressing the level of KANK3

Pterostilbene exerts anti-lung squamous cell carcinoma function by suppressing the level of KANK3

Early detection of lung squamous cell carcinoma (LUSC) has a significant impact on clinical outcomes, and pterostilbene (PT) is a natural compound with promising anti-oncogenic activities. This study aimed to identify potential LUSC biomarkers through a series of bioinformatic analyses and clinical verification and explored the interaction between PT and selected biomarkers during the treatment of LUSC. The analysis of the expression profile of the clinical samples of LUSC was performed to identify dysexpressed genes (DEGs) and validated by IHC. The role of KANK3 in the anti-LUSC effects of PT was assessed with a series of in vitro and in vivo assays. 4335 DEGs were identified, including 1851 upregulated genes and 2484 downregulated genes. Survival analysis showed that KANK3 was significantly higher in patients with LUSC with an advanced tumor stage. In in vitro assays, PT suppressed cell viability, induced apoptosis, and inhibited migration and invasion in LUSC cell lines, which was associated with downregulation of KANK3. After the reinduction of the KANK3 level in LUSC cells, the anti-LUSC function of PT was impaired. In mice model, reinduction of KANK3 increased tumor growth and metastasis even under the treatment of PT. The findings outlined in the current study indicated that PT exerted anti-LUSC function in a KANK3 inhibition-dependent manner.

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来源期刊
Chemical Biology & Drug Design
Chemical Biology & Drug Design 医学-生化与分子生物学
CiteScore
5.10
自引率
3.30%
发文量
164
审稿时长
4.4 months
期刊介绍: Chemical Biology & Drug Design is a peer-reviewed scientific journal that is dedicated to the advancement of innovative science, technology and medicine with a focus on the multidisciplinary fields of chemical biology and drug design. It is the aim of Chemical Biology & Drug Design to capture significant research and drug discovery that highlights new concepts, insight and new findings within the scope of chemical biology and drug design.
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