Alexandra Vozárová, Veronika Šmátralová, Renáta Fľaková, Milan Seman
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引用次数: 0
摘要
在布拉迪斯拉发的多瑙河地表水中测定了霍乱弧菌、米氏弧菌和弗洛维亚弧菌,它们是弧菌属重要的水生人类病原体。通过生化检验对分离物进行了鉴定。环境分离物的生化特征可能与参考菌株和临床菌株略有不同,这反映在差异率上,而在我们的案例中,差异率并不充分。为了确认表型鉴定,我们采用了一种名为扩增核糖体 DNA 限制分析的分子方法。22 个分离物中有 19 个的种属身份得到确认,3 个被误认的分离物的种属身份没有改变。利用名为 ompW 的物种特异性基因进行聚合酶链反应,确认了所有霍乱弧菌分离物的身份。此外,还确定了这些分离株的主要毒力因子。没有一个分离株具有诱发霍乱所需的全部三种毒力因子。
Occurrence, isolation, and identification of emergent pathogens of taxon Vibrio from the Danube river in Bratislava city, Slovakia
The occurrence of Vibrio cholerae, Vibrio metschnikovii and Vibrio fluvialis, important aquatic human pathogens of genus Vibrio, were determined in the surface water of the Danube River in Bratislava. The isolates were identified by biochemical tests. Biochemical characteristics of environmental isolates could be slightly different from reference and clinical strains which is reflected in dissimilarity rate, which was in our case not sufficient. A molecular method called amplified ribosomal DNA restriction analysis was used for confirmation of phenotypic identification. The species identity of 19 out of 22 isolates was confirmed, in the case of 3 misidentified ones, the identity of the genus did not change. The identity of all V. cholerae isolates was confirmed by PCR using species-specific gene called ompW. Also, main virulence factors of these isolates were determined. None of the isolates possessed all three virulence factors necessary for the induction of cholera.
期刊介绍:
Established in 1946, Biologia publishes high-quality research papers in the fields of microbial, plant and animal sciences. Microbial sciences papers span all aspects of Bacteria, Archaea and microbial Eucarya including biochemistry, cellular and molecular biology, genomics, proteomics and bioinformatics. Plant sciences topics include fundamental research in taxonomy, geobotany, genetics and all fields of experimental botany including cellular, whole-plant and community physiology. Zoology coverage includes animal systematics and taxonomy, morphology, ecology and physiology from cellular to molecular level.